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Radix Tetrastigma flavonoid ameliorates inflammation and prolongs the lifespan of Caenorhabditis elegans through JNK, p38 and Nrf2 pathways.
Free Radic Res. 2019 May; 53(5):562-573.FR

Abstract

The main flavonoid components of Radix Tetrastigma (RTF) were extracted and identified by UPLC-TOF/MS. In vitro, RTF prevented inflammation in RAW 264.7 cells by suppressing morphological (both cell and nucleus) changes, and decreasing nitric oxide (NO), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) contents. Exposure to LPS also leads to oxidant damage, and RTF alleviated damage to mitochondria, decreased O2- accumulation, and restored the glutathione level. RTF intervention decreased the expression of c-Jun N-terminal kinase (JNK) and p38 phosphorylation, accompanied by downregulation of nuclear factor erythroid 2-related factor 2 (Nrf2) and forkhead box protein O1 (FoxO1). In vivo, aging of Caenorhabditis elegans (C. elegans) by paraquat (PQ) was observed through lifespan, lipofuscin, and enzyme analysis. RTF protected against damage in N2 worms but not in daf-16 mutants. Gene expression was further assessed, and p38/PMK-1 and Nrf2/SKN-1 expression in worms was suppressed by PQ, which was reversed by RTF treatment. Together, these results suggested that RTF could help ameliorate inflammation-induced damage through JNK, p38 and Nrf2 pathways.

Authors+Show Affiliations

a Department of Food Science and Nutrition , Zhejiang University , Hangzhou , People's Republic of China. b Zhejiang Key Laboratory for Agro-Food Processing , Zhejiang University , Hangzhou , People's Republic of China. c Fuli Institute of Food Science , Zhejiang University , Hangzhou , People's Republic of China.a Department of Food Science and Nutrition , Zhejiang University , Hangzhou , People's Republic of China. b Zhejiang Key Laboratory for Agro-Food Processing , Zhejiang University , Hangzhou , People's Republic of China. c Fuli Institute of Food Science , Zhejiang University , Hangzhou , People's Republic of China.a Department of Food Science and Nutrition , Zhejiang University , Hangzhou , People's Republic of China. b Zhejiang Key Laboratory for Agro-Food Processing , Zhejiang University , Hangzhou , People's Republic of China. c Fuli Institute of Food Science , Zhejiang University , Hangzhou , People's Republic of China.a Department of Food Science and Nutrition , Zhejiang University , Hangzhou , People's Republic of China. b Zhejiang Key Laboratory for Agro-Food Processing , Zhejiang University , Hangzhou , People's Republic of China. c Fuli Institute of Food Science , Zhejiang University , Hangzhou , People's Republic of China.d Shanghai Zhengyue Enterprise Management Co, Ltd, Putuo District , Shanghai , People's Republic of China.a Department of Food Science and Nutrition , Zhejiang University , Hangzhou , People's Republic of China. b Zhejiang Key Laboratory for Agro-Food Processing , Zhejiang University , Hangzhou , People's Republic of China. c Fuli Institute of Food Science , Zhejiang University , Hangzhou , People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31039619

Citation

Li, Yonglu, et al. "Radix Tetrastigma Flavonoid Ameliorates Inflammation and Prolongs the Lifespan of Caenorhabditis Elegans Through JNK, P38 and Nrf2 Pathways." Free Radical Research, vol. 53, no. 5, 2019, pp. 562-573.
Li Y, Chu Q, Liu Y, et al. Radix Tetrastigma flavonoid ameliorates inflammation and prolongs the lifespan of Caenorhabditis elegans through JNK, p38 and Nrf2 pathways. Free Radic Res. 2019;53(5):562-573.
Li, Y., Chu, Q., Liu, Y., Ye, X., Jiang, Y., & Zheng, X. (2019). Radix Tetrastigma flavonoid ameliorates inflammation and prolongs the lifespan of Caenorhabditis elegans through JNK, p38 and Nrf2 pathways. Free Radical Research, 53(5), 562-573. https://doi.org/10.1080/10715762.2019.1613534
Li Y, et al. Radix Tetrastigma Flavonoid Ameliorates Inflammation and Prolongs the Lifespan of Caenorhabditis Elegans Through JNK, P38 and Nrf2 Pathways. Free Radic Res. 2019;53(5):562-573. PubMed PMID: 31039619.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Radix Tetrastigma flavonoid ameliorates inflammation and prolongs the lifespan of Caenorhabditis elegans through JNK, p38 and Nrf2 pathways. AU - Li,Yonglu, AU - Chu,Qiang, AU - Liu,Yangyang, AU - Ye,Xiang, AU - Jiang,Yong, AU - Zheng,Xiaodong, Y1 - 2019/05/16/ PY - 2019/5/2/pubmed PY - 2019/5/2/medline PY - 2019/5/2/entrez KW - KW - MAPKs KW - Nrf2 KW - Radix Tetrastigma KW - inflammation KW - oxidation resistance SP - 562 EP - 573 JF - Free radical research JO - Free Radic. Res. VL - 53 IS - 5 N2 - The main flavonoid components of Radix Tetrastigma (RTF) were extracted and identified by UPLC-TOF/MS. In vitro, RTF prevented inflammation in RAW 264.7 cells by suppressing morphological (both cell and nucleus) changes, and decreasing nitric oxide (NO), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) contents. Exposure to LPS also leads to oxidant damage, and RTF alleviated damage to mitochondria, decreased O2- accumulation, and restored the glutathione level. RTF intervention decreased the expression of c-Jun N-terminal kinase (JNK) and p38 phosphorylation, accompanied by downregulation of nuclear factor erythroid 2-related factor 2 (Nrf2) and forkhead box protein O1 (FoxO1). In vivo, aging of Caenorhabditis elegans (C. elegans) by paraquat (PQ) was observed through lifespan, lipofuscin, and enzyme analysis. RTF protected against damage in N2 worms but not in daf-16 mutants. Gene expression was further assessed, and p38/PMK-1 and Nrf2/SKN-1 expression in worms was suppressed by PQ, which was reversed by RTF treatment. Together, these results suggested that RTF could help ameliorate inflammation-induced damage through JNK, p38 and Nrf2 pathways. SN - 1029-2470 UR - https://www.unboundmedicine.com/medline/citation/31039619/Radix_Tetrastigma_flavonoid_ameliorates_inflammation_and_prolongs_the_lifespan_of_Caenorhabditis_elegans_through_JNK_p38_and_Nrf2_pathways_ L2 - http://www.tandfonline.com/doi/full/10.1080/10715762.2019.1613534 DB - PRIME DP - Unbound Medicine ER -