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Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl Coenzyme A.
Methods Mol Biol 2019; 1991:23-32MM

Abstract

Protein acetylation has emerged as a common modification that modulates multiple aspects of protein function, including localization, stability, and protein-protein interactions. It is increasingly evident that protein acetylation significantly impacts the outcome of host-microbe interactions. In order to characterize novel putative acetyltransferase enzymes and their substrates, we describe a simple protocol for the detection of acetyltransferase activity in vitro. Purified proteins are incubated with 14C-acetyl CoA and separated electrophoretically, and acetylated proteins are detected by phosphorimaging or autoradiography.

Authors+Show Affiliations

Department of Plant and Microbial Biology, University of California-Berkeley, Berkeley, CA, USA.Department of Plant and Microbial Biology, University of California-Berkeley, Berkeley, CA, USA. jdlewis@berkeley.edu. Plant Gene Expression Center, United States Department of Agriculture, Albany, CA, USA. jdlewis@berkeley.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31041759

Citation

Schreiber, Karl J., and Jennifer D. Lewis. "Protein Acetylation in Pathogen Virulence and Host Defense: in Vitro Detection of Protein Acetylation By Radiolabeled Acetyl Coenzyme A." Methods in Molecular Biology (Clifton, N.J.), vol. 1991, 2019, pp. 23-32.
Schreiber KJ, Lewis JD. Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl Coenzyme A. Methods Mol Biol. 2019;1991:23-32.
Schreiber, K. J., & Lewis, J. D. (2019). Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl Coenzyme A. Methods in Molecular Biology (Clifton, N.J.), 1991, pp. 23-32. doi:10.1007/978-1-4939-9458-8_3.
Schreiber KJ, Lewis JD. Protein Acetylation in Pathogen Virulence and Host Defense: in Vitro Detection of Protein Acetylation By Radiolabeled Acetyl Coenzyme A. Methods Mol Biol. 2019;1991:23-32. PubMed PMID: 31041759.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl Coenzyme A. AU - Schreiber,Karl J, AU - Lewis,Jennifer D, PY - 2019/5/2/entrez KW - 14C-acetyl CoA KW - In vitro assay KW - Pathogen KW - Protein acetylation KW - Radiolabeling SP - 23 EP - 32 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol. Biol. VL - 1991 N2 - Protein acetylation has emerged as a common modification that modulates multiple aspects of protein function, including localization, stability, and protein-protein interactions. It is increasingly evident that protein acetylation significantly impacts the outcome of host-microbe interactions. In order to characterize novel putative acetyltransferase enzymes and their substrates, we describe a simple protocol for the detection of acetyltransferase activity in vitro. Purified proteins are incubated with 14C-acetyl CoA and separated electrophoretically, and acetylated proteins are detected by phosphorimaging or autoradiography. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/31041759/Protein_Acetylation_in_Pathogen_Virulence_and_Host_Defense:_In_Vitro_Detection_of_Protein_Acetylation_by_Radiolabeled_Acetyl_Coenzyme_A L2 - https://dx.doi.org/10.1007/978-1-4939-9458-8_3 DB - PRIME DP - Unbound Medicine ER -