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RNA-Catalyzed Polymerization of Deoxyribose, Threose, and Arabinose Nucleic Acids.
ACS Synth Biol 2019; 8(5):955-961AS

Abstract

An RNA-dependent RNA polymerase ribozyme that was highly optimized through in vitro evolution for the ability to copy a broad range of template sequences exhibits promiscuity toward other nucleic acids and nucleic acid analogues, including DNA, threose nucleic acid (TNA), and arabinose nucleic acid (ANA). By operating on various RNA templates, the ribozyme catalyzes multiple successive additions of DNA, TNA, or ANA monomers, although with reduced efficiency compared to RNA monomers. The ribozyme can also copy DNA or TNA templates to complementary RNAs, and to a lesser extent it can operate when both the template and product strands are composed of DNA, TNA, or ANA. These results suggest that polymerase ribozymes, which are thought to have replicated RNA genomes during the early history of life, could have transferred RNA-based genetic information to and from DNA, enabling the emergence of DNA genomes prior to the emergence of proteins. In addition, genetic systems based on nucleic acid-like molecules, which have been proposed as precursors or contemporaries of RNA-based life, could have been operated upon by a promiscuous polymerase ribozyme, thus enabling the evolutionary transition between early genetic systems.

Authors+Show Affiliations

The Salk Institute , 10010 North Torrey Pines Road , La Jolla , California 92037 , United States.Departments of Pharmaceutical Sciences, Chemistry, and Molecular Biology and Biochemistry , University of California , Irvine , California 92697 , United States.Departments of Pharmaceutical Sciences, Chemistry, and Molecular Biology and Biochemistry , University of California , Irvine , California 92697 , United States.The Salk Institute , 10010 North Torrey Pines Road , La Jolla , California 92037 , United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31042360

Citation

Horning, David P., et al. "RNA-Catalyzed Polymerization of Deoxyribose, Threose, and Arabinose Nucleic Acids." ACS Synthetic Biology, vol. 8, no. 5, 2019, pp. 955-961.
Horning DP, Bala S, Chaput JC, et al. RNA-Catalyzed Polymerization of Deoxyribose, Threose, and Arabinose Nucleic Acids. ACS Synth Biol. 2019;8(5):955-961.
Horning, D. P., Bala, S., Chaput, J. C., & Joyce, G. F. (2019). RNA-Catalyzed Polymerization of Deoxyribose, Threose, and Arabinose Nucleic Acids. ACS Synthetic Biology, 8(5), pp. 955-961. doi:10.1021/acssynbio.9b00044.
Horning DP, et al. RNA-Catalyzed Polymerization of Deoxyribose, Threose, and Arabinose Nucleic Acids. ACS Synth Biol. 2019 May 17;8(5):955-961. PubMed PMID: 31042360.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RNA-Catalyzed Polymerization of Deoxyribose, Threose, and Arabinose Nucleic Acids. AU - Horning,David P, AU - Bala,Saikat, AU - Chaput,John C, AU - Joyce,Gerald F, Y1 - 2019/05/03/ PY - 2019/5/3/pubmed PY - 2019/5/3/medline PY - 2019/5/3/entrez KW - RNA world KW - XNA KW - origins of life KW - polymerase KW - reverse transcriptase KW - ribozyme SP - 955 EP - 961 JF - ACS synthetic biology JO - ACS Synth Biol VL - 8 IS - 5 N2 - An RNA-dependent RNA polymerase ribozyme that was highly optimized through in vitro evolution for the ability to copy a broad range of template sequences exhibits promiscuity toward other nucleic acids and nucleic acid analogues, including DNA, threose nucleic acid (TNA), and arabinose nucleic acid (ANA). By operating on various RNA templates, the ribozyme catalyzes multiple successive additions of DNA, TNA, or ANA monomers, although with reduced efficiency compared to RNA monomers. The ribozyme can also copy DNA or TNA templates to complementary RNAs, and to a lesser extent it can operate when both the template and product strands are composed of DNA, TNA, or ANA. These results suggest that polymerase ribozymes, which are thought to have replicated RNA genomes during the early history of life, could have transferred RNA-based genetic information to and from DNA, enabling the emergence of DNA genomes prior to the emergence of proteins. In addition, genetic systems based on nucleic acid-like molecules, which have been proposed as precursors or contemporaries of RNA-based life, could have been operated upon by a promiscuous polymerase ribozyme, thus enabling the evolutionary transition between early genetic systems. SN - 2161-5063 UR - https://www.unboundmedicine.com/medline/citation/31042360/RNA_Catalyzed_Polymerization_of_Deoxyribose_Threose_and_Arabinose_Nucleic_Acids_ L2 - https://dx.doi.org/10.1021/acssynbio.9b00044 DB - PRIME DP - Unbound Medicine ER -