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Evidence for infection but not transmission of Zika virus by Aedes albopictus (Diptera: Culicidae) from Spain.
Parasit Vectors 2019; 12(1):204PV

Abstract

BACKGROUND

A number of mosquito-borne viruses such as dengue virus (DENV), Usutu virus (USUV), West Nile virus (WNV) are autochthonously transmitted in Europe and six invasive mosquito species have been detected in this temperate region. This has increased the risk for the emergence of further mosquito-borne diseases. However, there is a paucity of information on whether European populations of invasive mosquito species are competent to transmit arboviruses. In this study, the susceptibility of Aedes albopictus originating from Spain and a laboratory-adapted colony of Aedes aegypti, was assessed for infection with, and transmission of Zika virus (ZIKV). Vertical transmission in both species was also assessed.

METHODS

Aedes albopictus colonised from eggs collected in Spain and an existing colony of Ae. aegypti were fed infectious blood meals containing ZIKV (Polynesian strain) at 1.6 × 107 PFU/ml. Blood-fed mosquitoes were separated and maintained at 20 °C or 25 °C. Legs, saliva and bodies were sampled from specimens at 7, 14 and 21 days post-infection (dpi) in order to determine infection, dissemination and transmission rates. All samples were analysed by real-time RT-PCR using primers targeting the ZIKV NS1 gene.

RESULTS

At 14 dpi and 21 dpi, ZIKV RNA was detected in the bodies of both species at both temperatures. However, live virus only was detected in the saliva of Ae. aegypti at 25 °C with a transmission rate of 44%. No evidence for virus expectoration was obtained for Ae. albopictus under any condition. Notably, ZIKV RNA was not detectable in the saliva of Ae. aegypti at 20 °C after 21 days. No vertical transmission of ZIKV was detected in this study.

CONCLUSIONS

Experimental infection of Ae. albopictus colonized from Spain with ZIKV did not result in expectoration of virus in saliva in contrast to results for Ae. aegypti. No evidence of vertical transmission of virus was observed in this study. This suggests that this strain of Ae. albopictus is not competent for ZIKV transmission under the conditions tested.

Authors+Show Affiliations

Wildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, UK. luis.hernandez-triana@apha.gov.uk.Wildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, UK.Department of Animal Pathology, Faculty of Veterinary Medicine, University of Zaragoza, Zaragoza, Spain.Center for Rickettsiosis and Vector-Borne Diseases Group, Hospital Universitario San Pedro-CIBIR, Logroño, Spain.Department of Animal Pathology, Faculty of Veterinary Medicine, University of Zaragoza, Zaragoza, Spain.Wildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, UK.Wildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, UK.Public Health England, Porton Down, Salisbury, SP4 0JG, UK.Wildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, UK. Faculty of Health and Medicine, University of Surrey, Guildford, Surrey, GU27XH, UK.Wildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, UK.Wildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey, KT15 3NB, UK. Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31053164

Citation

Hernández-Triana, Luis M., et al. "Evidence for Infection but Not Transmission of Zika Virus By Aedes Albopictus (Diptera: Culicidae) From Spain." Parasites & Vectors, vol. 12, no. 1, 2019, p. 204.
Hernández-Triana LM, Barrero E, Delacour-Estrella S, et al. Evidence for infection but not transmission of Zika virus by Aedes albopictus (Diptera: Culicidae) from Spain. Parasit Vectors. 2019;12(1):204.
Hernández-Triana, L. M., Barrero, E., Delacour-Estrella, S., Ruiz-Arrondo, I., Lucientes, J., Fernández de Marco, M. D. M., ... Fooks, A. R. (2019). Evidence for infection but not transmission of Zika virus by Aedes albopictus (Diptera: Culicidae) from Spain. Parasites & Vectors, 12(1), p. 204. doi:10.1186/s13071-019-3467-y.
Hernández-Triana LM, et al. Evidence for Infection but Not Transmission of Zika Virus By Aedes Albopictus (Diptera: Culicidae) From Spain. Parasit Vectors. 2019 May 3;12(1):204. PubMed PMID: 31053164.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence for infection but not transmission of Zika virus by Aedes albopictus (Diptera: Culicidae) from Spain. AU - Hernández-Triana,Luis M, AU - Barrero,Elsa, AU - Delacour-Estrella,Sarah, AU - Ruiz-Arrondo,Ignacio, AU - Lucientes,Javier, AU - Fernández de Marco,Maria Del Mar, AU - Thorne,Leigh, AU - Lumley,Sarah, AU - Johnson,Nicholas, AU - Mansfield,Karen L, AU - Fooks,Anthony R, Y1 - 2019/05/03/ PY - 2019/01/20/received PY - 2019/04/29/accepted PY - 2019/5/5/entrez PY - 2019/5/6/pubmed PY - 2019/6/27/medline KW - Aedes aegypti KW - Aedes albopictus KW - Spain KW - Vector competence KW - Zika virus SP - 204 EP - 204 JF - Parasites & vectors JO - Parasit Vectors VL - 12 IS - 1 N2 - BACKGROUND: A number of mosquito-borne viruses such as dengue virus (DENV), Usutu virus (USUV), West Nile virus (WNV) are autochthonously transmitted in Europe and six invasive mosquito species have been detected in this temperate region. This has increased the risk for the emergence of further mosquito-borne diseases. However, there is a paucity of information on whether European populations of invasive mosquito species are competent to transmit arboviruses. In this study, the susceptibility of Aedes albopictus originating from Spain and a laboratory-adapted colony of Aedes aegypti, was assessed for infection with, and transmission of Zika virus (ZIKV). Vertical transmission in both species was also assessed. METHODS: Aedes albopictus colonised from eggs collected in Spain and an existing colony of Ae. aegypti were fed infectious blood meals containing ZIKV (Polynesian strain) at 1.6 × 107 PFU/ml. Blood-fed mosquitoes were separated and maintained at 20 °C or 25 °C. Legs, saliva and bodies were sampled from specimens at 7, 14 and 21 days post-infection (dpi) in order to determine infection, dissemination and transmission rates. All samples were analysed by real-time RT-PCR using primers targeting the ZIKV NS1 gene. RESULTS: At 14 dpi and 21 dpi, ZIKV RNA was detected in the bodies of both species at both temperatures. However, live virus only was detected in the saliva of Ae. aegypti at 25 °C with a transmission rate of 44%. No evidence for virus expectoration was obtained for Ae. albopictus under any condition. Notably, ZIKV RNA was not detectable in the saliva of Ae. aegypti at 20 °C after 21 days. No vertical transmission of ZIKV was detected in this study. CONCLUSIONS: Experimental infection of Ae. albopictus colonized from Spain with ZIKV did not result in expectoration of virus in saliva in contrast to results for Ae. aegypti. No evidence of vertical transmission of virus was observed in this study. This suggests that this strain of Ae. albopictus is not competent for ZIKV transmission under the conditions tested. SN - 1756-3305 UR - https://www.unboundmedicine.com/medline/citation/31053164/Evidence_for_infection_but_not_transmission_of_Zika_virus_by_Aedes_albopictus__Diptera:_Culicidae__from_Spain_ L2 - https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-019-3467-y DB - PRIME DP - Unbound Medicine ER -