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Preserved Cerebral Oxygen Metabolism in Astrocytic Dysfunction: A Combination Study of 15O-Gas PET with 14C-Acetate Autoradiography.
Brain Sci 2019; 9(5)BS

Abstract

Fluorocitrate (FC) is a specific metabolic inhibitor of the tricarboxylic acid (TCA) cycle in astrocytes. The purpose of this study was to evaluate whether inhibition of the astrocyte TCA cycle by FC would affect the oxygen metabolism in the rat brain. At 4 h after the intracranial FC injection, the rats (n = 9) were investigated by 15O-labeled gas PET to measure the cerebral blood flow (CBF), the cerebral metabolic rate of oxygen (CMRO2), oxygen extraction fraction (OEF), and cerebral blood volume (CBV). After the 15O-gas PET, the rats were given an intravenous injection of 14C-acetate for autoradiography. 15O-gas PET showed no significant differences in any of the measured parameters between the ipsilateral and contralateral striatum (high dose group: CBF (54.4 ± 8.8 and 55.3 ± 11.6 mL/100mL/min), CMRO2 (7.0 ± 0.9 and 7.1 ± 1.2 mL/100mL/min), OEF (72.0 ± 8.9 and 70.8 ± 8.2%), and CBV (4.1 ± 0.8 and 4.2 ± 0.9 mL/100mL), respectively). In contrast, the 14C-acetate autoradiography revealed a significant inhibition of the astrocyte metabolism in the ipsilateral striatum. The regional cerebral oxygen consumption as well as the hemodynamic parameters were maintained even in the face of inhibition of the astrocyte TCA cycle metabolism in the rat brain.

Authors+Show Affiliations

Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine, Suita 565-0871, Japan. carlamari_md@yahoo.com.Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine, Suita 565-0871, Japan. watabe@tracer.med.osaka-u.ac.jp.Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine, Suita 565-0871, Japan. liu@tracer.med.osaka-u.ac.jp.Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine, Suita 565-0871, Japan. romanov@tracer.med.osaka-u.ac.jp.Department of Molecular Imaging in Medicine, Osaka University Graduate School of Medicine,Suita 565-0871, Japan. ykanai@mi.med.osaka-u.ac.jp.Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine, Suita 565-0871, Japan. horitsugi@tracer.med.osaka-u.ac.jp.Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine, Suita 565-0871, Japan. kato@tracer.med.osaka-u.ac.jp.Department of Molecular Imaging in Medicine, Osaka University Graduate School of Medicine,Suita 565-0871, Japan. eku@tracer.med.osaka-u.ac.jp.Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine, Suita 565-0871, Japan. hatazawa@tracer.med.osaka-u.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31058865

Citation

Macaisa, Carla Mari, et al. "Preserved Cerebral Oxygen Metabolism in Astrocytic Dysfunction: a Combination Study of 15O-Gas PET With 14C-Acetate Autoradiography." Brain Sciences, vol. 9, no. 5, 2019.
Macaisa CM, Watabe T, Liu Y, et al. Preserved Cerebral Oxygen Metabolism in Astrocytic Dysfunction: A Combination Study of 15O-Gas PET with 14C-Acetate Autoradiography. Brain Sci. 2019;9(5).
Macaisa, C. M., Watabe, T., Liu, Y., Romanov, V., Kanai, Y., Horitsugi, G., ... Hatazawa, J. (2019). Preserved Cerebral Oxygen Metabolism in Astrocytic Dysfunction: A Combination Study of 15O-Gas PET with 14C-Acetate Autoradiography. Brain Sciences, 9(5), doi:10.3390/brainsci9050101.
Macaisa CM, et al. Preserved Cerebral Oxygen Metabolism in Astrocytic Dysfunction: a Combination Study of 15O-Gas PET With 14C-Acetate Autoradiography. Brain Sci. 2019 May 3;9(5) PubMed PMID: 31058865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preserved Cerebral Oxygen Metabolism in Astrocytic Dysfunction: A Combination Study of 15O-Gas PET with 14C-Acetate Autoradiography. AU - Macaisa,Carla Mari, AU - Watabe,Tadashi, AU - Liu,Yuwei, AU - Romanov,Victor, AU - Kanai,Yasukazu, AU - Horitsugi,Genki, AU - Kato,Hiroki, AU - Shimosegawa,Eku, AU - Hatazawa,Jun, Y1 - 2019/05/03/ PY - 2019/03/20/received PY - 2019/04/24/revised PY - 2019/04/29/accepted PY - 2019/5/7/entrez PY - 2019/5/7/pubmed PY - 2019/5/7/medline KW - 14C-acetate KW - 15O-gas PET KW - TCA cycle KW - astrocyte KW - cerebral blood flow KW - cerebral metabolic rate of oxygen KW - fluorocitrate KW - oxygen consumption JF - Brain sciences JO - Brain Sci VL - 9 IS - 5 N2 - Fluorocitrate (FC) is a specific metabolic inhibitor of the tricarboxylic acid (TCA) cycle in astrocytes. The purpose of this study was to evaluate whether inhibition of the astrocyte TCA cycle by FC would affect the oxygen metabolism in the rat brain. At 4 h after the intracranial FC injection, the rats (n = 9) were investigated by 15O-labeled gas PET to measure the cerebral blood flow (CBF), the cerebral metabolic rate of oxygen (CMRO2), oxygen extraction fraction (OEF), and cerebral blood volume (CBV). After the 15O-gas PET, the rats were given an intravenous injection of 14C-acetate for autoradiography. 15O-gas PET showed no significant differences in any of the measured parameters between the ipsilateral and contralateral striatum (high dose group: CBF (54.4 ± 8.8 and 55.3 ± 11.6 mL/100mL/min), CMRO2 (7.0 ± 0.9 and 7.1 ± 1.2 mL/100mL/min), OEF (72.0 ± 8.9 and 70.8 ± 8.2%), and CBV (4.1 ± 0.8 and 4.2 ± 0.9 mL/100mL), respectively). In contrast, the 14C-acetate autoradiography revealed a significant inhibition of the astrocyte metabolism in the ipsilateral striatum. The regional cerebral oxygen consumption as well as the hemodynamic parameters were maintained even in the face of inhibition of the astrocyte TCA cycle metabolism in the rat brain. SN - 2076-3425 UR - https://www.unboundmedicine.com/medline/citation/31058865/Preserved_Cerebral_Oxygen_Metabolism_in_Astrocytic_Dysfunction:_A_Combination_Study_of_15O-Gas_PET_with_14C-Acetate_Autoradiography L2 - http://www.mdpi.com/resolver?pii=brainsci9050101 DB - PRIME DP - Unbound Medicine ER -