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Introgression of a Novel Ug99-Effective Stem Rust Resistance Gene into Wheat and Development of Dasypyrum villosum Chromosome-Specific Markers via Genotyping-by-Sequencing (GBS).
Plant Dis. 2019 Jun; 103(6):1068-1074.PD

Abstract

Dasypyrum villosum is a wild relative of common wheat (Triticum aestivum L.) with resistance to Puccinia graminis f. tritici, the causal agent of stem rust, including the highly virulent race TTKSK (Ug99). In order to transfer resistance, T. durum-D. villosum amphiploids were initially developed and used as a bridge to create wheat-D. villosum introgression lines. Conserved ortholog set (COS) markers were used to identify D. villosum chromosome introgression lines, which were then subjected to seedling P. graminis f. tritici resistance screening with race TTKSK. A COS marker-verified line carrying chromosome 2V with TTKSK resistance was further characterized by combined genomic in situ and fluorescent in situ analyses to confirm a monosomic substitution line MS2V(2D) (20″ + 1' 2V[2D]). This is the first report of stem rust resistance on 2V, which was temporarily designated as SrTA10276-2V. To facilitate the use of this gene in wheat improvement, a complete set of previously developed wheat-D. villosum disomic addition lines was subjected to genotyping-by-sequencing analysis to develop D. villosum chromosome-specific markers. On average, 350 markers per chromosome were identified. These markers can be used to develop diagnostic markers for D. villosum-derived genes of interest in wheat improvement.

Authors+Show Affiliations

1 Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164.1 Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164.1 Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164.2 United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory and Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108.3 Wheat Genetics Resource Center, Kansas State University, Manhattan, KS 66506.3 Wheat Genetics Resource Center, Kansas State University, Manhattan, KS 66506.4 USDA-ARS, Western Regional Small Grains Genotyping Laboratory and Department of Plant Pathology, Washington State University, Pullman, WA 99164.1 Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31063029

Citation

Ando, Kaori, et al. "Introgression of a Novel Ug99-Effective Stem Rust Resistance Gene Into Wheat and Development of Dasypyrum Villosum Chromosome-Specific Markers Via Genotyping-by-Sequencing (GBS)." Plant Disease, vol. 103, no. 6, 2019, pp. 1068-1074.
Ando K, Krishnan V, Rynearson S, et al. Introgression of a Novel Ug99-Effective Stem Rust Resistance Gene into Wheat and Development of Dasypyrum villosum Chromosome-Specific Markers via Genotyping-by-Sequencing (GBS). Plant Dis. 2019;103(6):1068-1074.
Ando, K., Krishnan, V., Rynearson, S., Rouse, M. N., Danilova, T., Friebe, B., See, D., & Pumphrey, M. O. (2019). Introgression of a Novel Ug99-Effective Stem Rust Resistance Gene into Wheat and Development of Dasypyrum villosum Chromosome-Specific Markers via Genotyping-by-Sequencing (GBS). Plant Disease, 103(6), 1068-1074. https://doi.org/10.1094/PDIS-05-18-0831-RE
Ando K, et al. Introgression of a Novel Ug99-Effective Stem Rust Resistance Gene Into Wheat and Development of Dasypyrum Villosum Chromosome-Specific Markers Via Genotyping-by-Sequencing (GBS). Plant Dis. 2019;103(6):1068-1074. PubMed PMID: 31063029.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Introgression of a Novel Ug99-Effective Stem Rust Resistance Gene into Wheat and Development of Dasypyrum villosum Chromosome-Specific Markers via Genotyping-by-Sequencing (GBS). AU - Ando,Kaori, AU - Krishnan,Vandhana, AU - Rynearson,Sheri, AU - Rouse,Matthew N, AU - Danilova,Tatiana, AU - Friebe,Bernd, AU - See,Deven, AU - Pumphrey,Michael O, Y1 - 2019/05/07/ PY - 2019/5/8/pubmed PY - 2019/8/8/medline PY - 2019/5/8/entrez KW - cereals and grains KW - field crops KW - fungi KW - techniques SP - 1068 EP - 1074 JF - Plant disease JO - Plant Dis VL - 103 IS - 6 N2 - Dasypyrum villosum is a wild relative of common wheat (Triticum aestivum L.) with resistance to Puccinia graminis f. tritici, the causal agent of stem rust, including the highly virulent race TTKSK (Ug99). In order to transfer resistance, T. durum-D. villosum amphiploids were initially developed and used as a bridge to create wheat-D. villosum introgression lines. Conserved ortholog set (COS) markers were used to identify D. villosum chromosome introgression lines, which were then subjected to seedling P. graminis f. tritici resistance screening with race TTKSK. A COS marker-verified line carrying chromosome 2V with TTKSK resistance was further characterized by combined genomic in situ and fluorescent in situ analyses to confirm a monosomic substitution line MS2V(2D) (20″ + 1' 2V[2D]). This is the first report of stem rust resistance on 2V, which was temporarily designated as SrTA10276-2V. To facilitate the use of this gene in wheat improvement, a complete set of previously developed wheat-D. villosum disomic addition lines was subjected to genotyping-by-sequencing analysis to develop D. villosum chromosome-specific markers. On average, 350 markers per chromosome were identified. These markers can be used to develop diagnostic markers for D. villosum-derived genes of interest in wheat improvement. SN - 0191-2917 UR - https://www.unboundmedicine.com/medline/citation/31063029/Introgression_of_a_Novel_Ug99_Effective_Stem_Rust_Resistance_Gene_into_Wheat_and_Development_of_Dasypyrum_villosum_Chromosome_Specific_Markers_via_Genotyping_by_Sequencing__GBS__ L2 - https://apsjournals.apsnet.org/doi/10.1094/PDIS-05-18-0831-RE?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -