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Diversity of Staphylococcus aureus clones in wild mammals in Aragon, Spain, with detection of MRSA ST130-mecC in wild rabbits.
J Appl Microbiol. 2019 Jul; 127(1):284-291.JA

Abstract

AIMS

To determine the Staphylococcus aureus carriage rate in wild mammals in Aragon, northern Spain, to analyse their antimicrobial resistance phenotype/genotype and to characterize the recovered isolates.

METHODS AND RESULTS

Nasal and rectal swabs of 103 mammals were collected in Aragón during the period 2012-2015. Antimicrobial susceptibility, the presence of antimicrobial resistance genes and virulence factors were investigated. Molecular characterization was carried out by spa, MLST, agr and SCCmec. Staphylococcus aureus were recovered from 23 animals (22%). Four of the 23 S. aureus were methicillin-resistant S. aureus (MRSA). Three MRSA were mecC-positive and were isolated from European rabbits and were typed as t843 (ascribed to CC130). The remaining MRSA was a mecA-carrying isolate from European hedgehog, typed as ST1-t386-SCCmecIVa-agrIII and it harboured the blaZ, erm(C), ant(6)-Ia and aph(3´)-IIIa resistance genes. A high diversity of spa-types was detected among the 19 methicillin-susceptible S. aureus isolates, which showed high susceptibility to the antimicrobials tested. The tst gene and different combinations of staphylococcal enterotoxins were found.

CONCLUSIONS

Staphylococcus aureus were detected in nasal and rectal samples of wild mammals. Wild rabbits could be a reservoir of mecC-MRSA.

SIGNIFICANCE AND IMPACT OF THE STUDY

This work provides information on the presence and characteristics of S. aureus from mammals in a defined geographic region in Spain.

Authors+Show Affiliations

Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.Departamento de Patología Animal, Universidad de Zaragoza, Zaragoza, Spain.Departamento de Patología Animal, Universidad de Zaragoza, Zaragoza, Spain.Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.Servicio de Microbiología, Hospital Universitario Miguel Servet, IIS Aragón, Zaragoza, Spain.Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31063623

Citation

Ruiz-Ripa, L, et al. "Diversity of Staphylococcus Aureus Clones in Wild Mammals in Aragon, Spain, With Detection of MRSA ST130-mecC in Wild Rabbits." Journal of Applied Microbiology, vol. 127, no. 1, 2019, pp. 284-291.
Ruiz-Ripa L, Alcalá L, Simón C, et al. Diversity of Staphylococcus aureus clones in wild mammals in Aragon, Spain, with detection of MRSA ST130-mecC in wild rabbits. J Appl Microbiol. 2019;127(1):284-291.
Ruiz-Ripa, L., Alcalá, L., Simón, C., Gómez, P., Mama, O. M., Rezusta, A., Zarazaga, M., & Torres, C. (2019). Diversity of Staphylococcus aureus clones in wild mammals in Aragon, Spain, with detection of MRSA ST130-mecC in wild rabbits. Journal of Applied Microbiology, 127(1), 284-291. https://doi.org/10.1111/jam.14301
Ruiz-Ripa L, et al. Diversity of Staphylococcus Aureus Clones in Wild Mammals in Aragon, Spain, With Detection of MRSA ST130-mecC in Wild Rabbits. J Appl Microbiol. 2019;127(1):284-291. PubMed PMID: 31063623.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity of Staphylococcus aureus clones in wild mammals in Aragon, Spain, with detection of MRSA ST130-mecC in wild rabbits. AU - Ruiz-Ripa,L, AU - Alcalá,L, AU - Simón,C, AU - Gómez,P, AU - Mama,O M, AU - Rezusta,A, AU - Zarazaga,M, AU - Torres,C, PY - 2019/02/09/received PY - 2019/04/05/revised PY - 2019/04/28/accepted PY - 2019/5/8/pubmed PY - 2019/8/15/medline PY - 2019/5/8/entrez KW - Staphylococcus aureus KW - CC130 KW - free-living animals KW - mecA-MRSA KW - mecC-MRSA SP - 284 EP - 291 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 127 IS - 1 N2 - AIMS: To determine the Staphylococcus aureus carriage rate in wild mammals in Aragon, northern Spain, to analyse their antimicrobial resistance phenotype/genotype and to characterize the recovered isolates. METHODS AND RESULTS: Nasal and rectal swabs of 103 mammals were collected in Aragón during the period 2012-2015. Antimicrobial susceptibility, the presence of antimicrobial resistance genes and virulence factors were investigated. Molecular characterization was carried out by spa, MLST, agr and SCCmec. Staphylococcus aureus were recovered from 23 animals (22%). Four of the 23 S. aureus were methicillin-resistant S. aureus (MRSA). Three MRSA were mecC-positive and were isolated from European rabbits and were typed as t843 (ascribed to CC130). The remaining MRSA was a mecA-carrying isolate from European hedgehog, typed as ST1-t386-SCCmecIVa-agrIII and it harboured the blaZ, erm(C), ant(6)-Ia and aph(3´)-IIIa resistance genes. A high diversity of spa-types was detected among the 19 methicillin-susceptible S. aureus isolates, which showed high susceptibility to the antimicrobials tested. The tst gene and different combinations of staphylococcal enterotoxins were found. CONCLUSIONS: Staphylococcus aureus were detected in nasal and rectal samples of wild mammals. Wild rabbits could be a reservoir of mecC-MRSA. SIGNIFICANCE AND IMPACT OF THE STUDY: This work provides information on the presence and characteristics of S. aureus from mammals in a defined geographic region in Spain. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/31063623/Diversity_of_Staphylococcus_aureus_clones_in_wild_mammals_in_Aragon_Spain_with_detection_of_MRSA_ST130_mecC_in_wild_rabbits_ L2 - https://doi.org/10.1111/jam.14301 DB - PRIME DP - Unbound Medicine ER -