Tags

Type your tag names separated by a space and hit enter

Outcomes of processing of bone marrow harvests for hematopoietic stem cell transplantation in pediatric patients utilizing a novel red blood cell sedimentation kit.
Transfusion 2019; 59(7):2375-2381T

Abstract

BACKGROUND

Bone marrow (BM) grafts are often subject to manipulation to minimize infusion volume, to deplete red blood cells (RBCs), to deplete plasma, and/or to achieve optimal concentration of nucleated cells (NCs) for freezing. Here, we report results of processing and infusion of four major ABO-incompatible BM grafts for allogeneic hematopoietic transplantation in pediatric patients.

CASES AND METHODS

Patients ranging in age from 5 months to 18 years received hematopoietic stem cell transplantation for benign or malignant indications. Allogeneic BM grafts were processed using PrepaCyte-CB kits, approved by the Food and Drug Administration for enrichment of mononuclear cells in cord blood units for hematopoietic transplantation. Resulting grafts were dosed and infused using standard institutional protocol. After transplantation patients were evaluated for hematologic recovery.

RESULTS

The PrepaCyte-CB processing method achieved on average 97.1% depletion of RBCs and 68.5% volume reduction, with a fairly efficient NC recovery (total NCs, 85.6%; white blood cells, 89.7%; CD34+ cells, 103.3%; and colony-forming unit cells, 87.5%). No microbial contamination was detectable in any of the processed grafts. Infusions were well tolerated, and all four patients achieved durable hematopoietic engraftment within expected time interval.

CONCLUSION

Our results suggest that PrepaCyte-CB is safe and effective for minimal processing of BM grafts. Engraftment outcomes were comparable with other published BM processing methods. In addition, NC recovery achieved using this method appears to exceed that of previously described sedimentation reagents such as hetastarch and Ficoll.

Authors+Show Affiliations

St. Louis Cord Blood Bank and Cellular Therapy Laboratory, SSM Health Cardinal Glennon Children's Hospital, St. Louis, Missouri. Department of Pediatrics, St Louis University School of Medicine, St Louis, Missouri. Department of Bone Marrow Transplantation & Cellular Therapy, St Jude Children's Research Hospital, Memphis, Tennessee.St. Louis Cord Blood Bank and Cellular Therapy Laboratory, SSM Health Cardinal Glennon Children's Hospital, St. Louis, Missouri.St. Louis Cord Blood Bank and Cellular Therapy Laboratory, SSM Health Cardinal Glennon Children's Hospital, St. Louis, Missouri. Department of Pediatrics, St Louis University School of Medicine, St Louis, Missouri.St. Louis Cord Blood Bank and Cellular Therapy Laboratory, SSM Health Cardinal Glennon Children's Hospital, St. Louis, Missouri. Department of Pediatrics, St Louis University School of Medicine, St Louis, Missouri.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31066469

Citation

Akel, Salem, et al. "Outcomes of Processing of Bone Marrow Harvests for Hematopoietic Stem Cell Transplantation in Pediatric Patients Utilizing a Novel Red Blood Cell Sedimentation Kit." Transfusion, vol. 59, no. 7, 2019, pp. 2375-2381.
Akel S, Murray C, Ferguson W, et al. Outcomes of processing of bone marrow harvests for hematopoietic stem cell transplantation in pediatric patients utilizing a novel red blood cell sedimentation kit. Transfusion. 2019;59(7):2375-2381.
Akel, S., Murray, C., Ferguson, W., & Babic, A. (2019). Outcomes of processing of bone marrow harvests for hematopoietic stem cell transplantation in pediatric patients utilizing a novel red blood cell sedimentation kit. Transfusion, 59(7), pp. 2375-2381. doi:10.1111/trf.15337.
Akel S, et al. Outcomes of Processing of Bone Marrow Harvests for Hematopoietic Stem Cell Transplantation in Pediatric Patients Utilizing a Novel Red Blood Cell Sedimentation Kit. Transfusion. 2019;59(7):2375-2381. PubMed PMID: 31066469.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Outcomes of processing of bone marrow harvests for hematopoietic stem cell transplantation in pediatric patients utilizing a novel red blood cell sedimentation kit. AU - Akel,Salem, AU - Murray,Christianna, AU - Ferguson,William, AU - Babic,Aleksandar, Y1 - 2019/05/08/ PY - 2019/02/15/received PY - 2019/04/09/revised PY - 2019/04/10/accepted PY - 2019/5/9/pubmed PY - 2019/5/9/medline PY - 2019/5/9/entrez SP - 2375 EP - 2381 JF - Transfusion JO - Transfusion VL - 59 IS - 7 N2 - BACKGROUND: Bone marrow (BM) grafts are often subject to manipulation to minimize infusion volume, to deplete red blood cells (RBCs), to deplete plasma, and/or to achieve optimal concentration of nucleated cells (NCs) for freezing. Here, we report results of processing and infusion of four major ABO-incompatible BM grafts for allogeneic hematopoietic transplantation in pediatric patients. CASES AND METHODS: Patients ranging in age from 5 months to 18 years received hematopoietic stem cell transplantation for benign or malignant indications. Allogeneic BM grafts were processed using PrepaCyte-CB kits, approved by the Food and Drug Administration for enrichment of mononuclear cells in cord blood units for hematopoietic transplantation. Resulting grafts were dosed and infused using standard institutional protocol. After transplantation patients were evaluated for hematologic recovery. RESULTS: The PrepaCyte-CB processing method achieved on average 97.1% depletion of RBCs and 68.5% volume reduction, with a fairly efficient NC recovery (total NCs, 85.6%; white blood cells, 89.7%; CD34+ cells, 103.3%; and colony-forming unit cells, 87.5%). No microbial contamination was detectable in any of the processed grafts. Infusions were well tolerated, and all four patients achieved durable hematopoietic engraftment within expected time interval. CONCLUSION: Our results suggest that PrepaCyte-CB is safe and effective for minimal processing of BM grafts. Engraftment outcomes were comparable with other published BM processing methods. In addition, NC recovery achieved using this method appears to exceed that of previously described sedimentation reagents such as hetastarch and Ficoll. SN - 1537-2995 UR - https://www.unboundmedicine.com/medline/citation/31066469/Outcomes_of_processing_of_bone_marrow_harvests_for_hematopoietic_stem_cell_transplantation_in_pediatric_patients_utilizing_a_novel_red_blood_cell_sedimentation_kit L2 - https://doi.org/10.1111/trf.15337 DB - PRIME DP - Unbound Medicine ER -