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Anti-hepatofibrosis effect of Allium senescens in activated hepatic stellate cells and thioacetamide-induced fibrosis rat model.
Pharm Biol. 2018 Dec; 56(1):632-642.PB

Abstract

CONTEXT

Allium senescens Linn. (Liliaceae) (ASL) has been traditionally used in Korea and other Asian countries for improving digestive and liver functions.

OBJECTIVE

The anti-hepatofibrosis effect of ASL ethanol extract in cellular and experimental fibrosis rat model was investigated.

MATERIALS AND METHODS

In vitro cell viability, cell cycle and apoptosis in hepatic stellate cells (HSCs) were studied using MTT assay, flow cytometry and Annexin V-FITC/PI staining. Thioacetamide (TAA; 200 mg/kg, i.p.)-induced liver fibrosis model using Sprague Dawley rats (n = 10) was developed in vivo by injecting TAA twice per week for 13 weeks. ASL (25 and 100 mg/kg) and silymarin (50 mg/kg) were administered through oral gavage 2 times per week from 7th to 13th week. Specific fibrotic-related biomarkers such as aspartate transaminase (AST), alanine transaminase (ALT), glutathione and hydroxyproline levels in serum were analyzed by spectrophotometer using commercial kits. Morphological, histopathological and fibrotic-related gene expression such as TGF-β, Col1α1 and α-SMA in liver tissues was estimated by hematoxylin and eosin staining, Picrosirius red stain and quantitative real-time polymerase chain reaction, respectively.

RESULTS

ASL (0.1 mg/mL) and silymarin (0.05 mg/mL) treatment induced apoptosis (4.06% and 8.67%) in activated HSC-T6 cells, compared with control group (3.7%). The altered morphology in activated primary HSCs was also restored by ASL (0.1 mg/mL) treatment. Further, ASL (100 and 25 mg/kg) ameliorated the TAA-induced altered fibrotic-related biomarkers, histopathological changes and fibrotic-related gene expression significantly (p < 0.05 ∼ p < 0.001).

CONCLUSIONS

ASL can potentially be developed as a therapeutic agent in the treatment of hepatic fibrosis.

Authors+Show Affiliations

a Department of Applied Life Science , Graduate School of Konkuk University , Chungju-si , Chungbuk , Republic of Korea.b Department of Biotechnology , College of Biomedical and Health Sciences, Konkuk University , Chungju-si , Chungbuk , Republic of Korea.a Department of Applied Life Science , Graduate School of Konkuk University , Chungju-si , Chungbuk , Republic of Korea.c Daesowon Food , Chungju-si , Chungbuk , Republic of Korea.d Department of Internal Medicine, School of Medicine , Konkuk University , Chungju , Chungbuk , Republic of Korea.e Department of Food Science and Engineering , Seowon University , Cheongju , Chingbuk , Republic of Korea.a Department of Applied Life Science , Graduate School of Konkuk University , Chungju-si , Chungbuk , Republic of Korea. b Department of Biotechnology , College of Biomedical and Health Sciences, Konkuk University , Chungju-si , Chungbuk , Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31070527

Citation

Shin, Gwang-Mo, et al. "Anti-hepatofibrosis Effect of Allium Senescens in Activated Hepatic Stellate Cells and Thioacetamide-induced Fibrosis Rat Model." Pharmaceutical Biology, vol. 56, no. 1, 2018, pp. 632-642.
Shin GM, Koppula S, Chae YJ, et al. Anti-hepatofibrosis effect of Allium senescens in activated hepatic stellate cells and thioacetamide-induced fibrosis rat model. Pharm Biol. 2018;56(1):632-642.
Shin, G. M., Koppula, S., Chae, Y. J., Kim, H. S., Lee, J. D., Kim, M. K., & Song, M. (2018). Anti-hepatofibrosis effect of Allium senescens in activated hepatic stellate cells and thioacetamide-induced fibrosis rat model. Pharmaceutical Biology, 56(1), 632-642. https://doi.org/10.1080/13880209.2018.1529801
Shin GM, et al. Anti-hepatofibrosis Effect of Allium Senescens in Activated Hepatic Stellate Cells and Thioacetamide-induced Fibrosis Rat Model. Pharm Biol. 2018;56(1):632-642. PubMed PMID: 31070527.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-hepatofibrosis effect of Allium senescens in activated hepatic stellate cells and thioacetamide-induced fibrosis rat model. AU - Shin,Gwang-Mo, AU - Koppula,Sushruta, AU - Chae,Yun-Jin, AU - Kim,Hyun-Su, AU - Lee,Jae-Dong, AU - Kim,Myong-Ki, AU - Song,MinDong, PY - 2019/5/10/entrez PY - 2019/5/10/pubmed PY - 2019/9/5/medline KW - Liver fibrosis KW - apoptosis KW - extracellular matrix KW - hydroxyproline KW - thioacetamide SP - 632 EP - 642 JF - Pharmaceutical biology JO - Pharm Biol VL - 56 IS - 1 N2 - CONTEXT: Allium senescens Linn. (Liliaceae) (ASL) has been traditionally used in Korea and other Asian countries for improving digestive and liver functions. OBJECTIVE: The anti-hepatofibrosis effect of ASL ethanol extract in cellular and experimental fibrosis rat model was investigated. MATERIALS AND METHODS: In vitro cell viability, cell cycle and apoptosis in hepatic stellate cells (HSCs) were studied using MTT assay, flow cytometry and Annexin V-FITC/PI staining. Thioacetamide (TAA; 200 mg/kg, i.p.)-induced liver fibrosis model using Sprague Dawley rats (n = 10) was developed in vivo by injecting TAA twice per week for 13 weeks. ASL (25 and 100 mg/kg) and silymarin (50 mg/kg) were administered through oral gavage 2 times per week from 7th to 13th week. Specific fibrotic-related biomarkers such as aspartate transaminase (AST), alanine transaminase (ALT), glutathione and hydroxyproline levels in serum were analyzed by spectrophotometer using commercial kits. Morphological, histopathological and fibrotic-related gene expression such as TGF-β, Col1α1 and α-SMA in liver tissues was estimated by hematoxylin and eosin staining, Picrosirius red stain and quantitative real-time polymerase chain reaction, respectively. RESULTS: ASL (0.1 mg/mL) and silymarin (0.05 mg/mL) treatment induced apoptosis (4.06% and 8.67%) in activated HSC-T6 cells, compared with control group (3.7%). The altered morphology in activated primary HSCs was also restored by ASL (0.1 mg/mL) treatment. Further, ASL (100 and 25 mg/kg) ameliorated the TAA-induced altered fibrotic-related biomarkers, histopathological changes and fibrotic-related gene expression significantly (p < 0.05 ∼ p < 0.001). CONCLUSIONS: ASL can potentially be developed as a therapeutic agent in the treatment of hepatic fibrosis. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/31070527/Anti_hepatofibrosis_effect_of_Allium_senescens_in_activated_hepatic_stellate_cells_and_thioacetamide_induced_fibrosis_rat_model_ L2 - https://www.tandfonline.com/doi/full/10.1080/13880209.2018.1529801 DB - PRIME DP - Unbound Medicine ER -