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Visfatin Promotes Monocyte Adhesion by Upregulating ICAM-1 and VCAM-1 Expression in Endothelial Cells via Activation of p38-PI3K-Akt Signaling and Subsequent ROS Production and IKK/NF-κB Activation.
Cell Physiol Biochem. 2019; 52(6):1398-1411.CP

Abstract

BACKGROUND/AIMS

Visfatin is known to act as a mediator in several metabolic disorders, such as obesity, diabetes, and cardiovascular diseases. This study aimed to investigate the effect of visfatin on the adhesion of THP-1 monocytes to human vascular endothelial cells and the underlying mechanism.

METHODS

Monocytes adhesion to endothelial cells was determined by using fluorescence-labeled monocytes. ICAM-1 and VCAM-1 expression in endothelial cells were measured by western blotting. Production of reactive oxygen species (ROS) was measured by using a fluorescent dye. The amounts of nuclear factor-kappa B (NF-κB) and phosphorylation of inhibitory factor of NF-κB (IκB) were determined by using western blot analysis. The translocation of NF-κB from the cytoplasm to the nucleus was determined by using immunofluorescence.

RESULTS

Here we showed that visfatin significantly caused the upregulation of intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in endothelial cells, as well as enhanced monocyte adhesion to endothelial cells. Moreover, we found that inhibition of PI3K, Akt, and p38 MAPK activation significantly prevented visfatin-enhanced expression of ICAM-1 and VCAM-1 and monocyte adhesion to endothelial cells. Visfatin enhanced ROS production and IKK/NF-кB activation and then led to upregulation of ICAM-1 and VCAM-1 and enhanced monocyte adhesion to endothelial cells. These effects were also p38/PI3K/Akt-dependent.

CONCLUSION

These results demonstrated that visfatin promoted monocyte-endothelial cell adhesion by increasing ICAM-1 and VCAM-1 expression via the activation of p38/PI3K/Akt signaling and downstream ROS production and IKK/NF-кB activation.

Authors+Show Affiliations

Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan.Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan.Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan. Division of Nephrology, Wen-Lin Hemodialysis Unit, Taipei Veterans General Hospital, Taipei, Taiwan.Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan.Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan.Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan.Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan.Division of Endocrinology and Metabolism, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan. Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan.Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan. Division of Endocrinology and Metabolism, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei, Taiwan. Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan.Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan. Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan. Department of Education and Research, Taipei City Hospital, Taipei, Taiwan, ccjuan@ym.edu.tw.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31075190

Citation

Lin, Yu-Ting, et al. "Visfatin Promotes Monocyte Adhesion By Upregulating ICAM-1 and VCAM-1 Expression in Endothelial Cells Via Activation of p38-PI3K-Akt Signaling and Subsequent ROS Production and IKK/NF-κB Activation." Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, vol. 52, no. 6, 2019, pp. 1398-1411.
Lin YT, Chen LK, Jian DY, et al. Visfatin Promotes Monocyte Adhesion by Upregulating ICAM-1 and VCAM-1 Expression in Endothelial Cells via Activation of p38-PI3K-Akt Signaling and Subsequent ROS Production and IKK/NF-κB Activation. Cell Physiol Biochem. 2019;52(6):1398-1411.
Lin, Y. T., Chen, L. K., Jian, D. Y., Hsu, T. C., Huang, W. C., Kuan, T. T., Wu, S. Y., Kwok, C. F., Ho, L. T., & Juan, C. C. (2019). Visfatin Promotes Monocyte Adhesion by Upregulating ICAM-1 and VCAM-1 Expression in Endothelial Cells via Activation of p38-PI3K-Akt Signaling and Subsequent ROS Production and IKK/NF-κB Activation. Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, 52(6), 1398-1411. https://doi.org/10.33594/000000098
Lin YT, et al. Visfatin Promotes Monocyte Adhesion By Upregulating ICAM-1 and VCAM-1 Expression in Endothelial Cells Via Activation of p38-PI3K-Akt Signaling and Subsequent ROS Production and IKK/NF-κB Activation. Cell Physiol Biochem. 2019;52(6):1398-1411. PubMed PMID: 31075190.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Visfatin Promotes Monocyte Adhesion by Upregulating ICAM-1 and VCAM-1 Expression in Endothelial Cells via Activation of p38-PI3K-Akt Signaling and Subsequent ROS Production and IKK/NF-κB Activation. AU - Lin,Yu-Ting, AU - Chen,Luen-Kui, AU - Jian,Deng-Yuan, AU - Hsu,Ting-Chia, AU - Huang,Wei-Chih, AU - Kuan,Tse-Ting, AU - Wu,Shao-Yun, AU - Kwok,Ching-Fai, AU - Ho,Low-Tone, AU - Juan,Chi-Chang, PY - 2017/04/28/received PY - 2019/05/06/accepted PY - 2019/5/11/entrez PY - 2019/5/11/pubmed PY - 2019/5/22/medline KW - Endothelial cell KW - ICAM-1 KW - Monocyte adhesion KW - VCAM-1 KW - Visfatin SP - 1398 EP - 1411 JF - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology JO - Cell. Physiol. Biochem. VL - 52 IS - 6 N2 - BACKGROUND/AIMS: Visfatin is known to act as a mediator in several metabolic disorders, such as obesity, diabetes, and cardiovascular diseases. This study aimed to investigate the effect of visfatin on the adhesion of THP-1 monocytes to human vascular endothelial cells and the underlying mechanism. METHODS: Monocytes adhesion to endothelial cells was determined by using fluorescence-labeled monocytes. ICAM-1 and VCAM-1 expression in endothelial cells were measured by western blotting. Production of reactive oxygen species (ROS) was measured by using a fluorescent dye. The amounts of nuclear factor-kappa B (NF-κB) and phosphorylation of inhibitory factor of NF-κB (IκB) were determined by using western blot analysis. The translocation of NF-κB from the cytoplasm to the nucleus was determined by using immunofluorescence. RESULTS: Here we showed that visfatin significantly caused the upregulation of intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in endothelial cells, as well as enhanced monocyte adhesion to endothelial cells. Moreover, we found that inhibition of PI3K, Akt, and p38 MAPK activation significantly prevented visfatin-enhanced expression of ICAM-1 and VCAM-1 and monocyte adhesion to endothelial cells. Visfatin enhanced ROS production and IKK/NF-кB activation and then led to upregulation of ICAM-1 and VCAM-1 and enhanced monocyte adhesion to endothelial cells. These effects were also p38/PI3K/Akt-dependent. CONCLUSION: These results demonstrated that visfatin promoted monocyte-endothelial cell adhesion by increasing ICAM-1 and VCAM-1 expression via the activation of p38/PI3K/Akt signaling and downstream ROS production and IKK/NF-кB activation. SN - 1421-9778 UR - https://www.unboundmedicine.com/medline/citation/31075190/Visfatin_Promotes_Monocyte_Adhesion_by_Upregulating_ICAM_1_and_VCAM_1_Expression_in_Endothelial_Cells_via_Activation_of_p38_PI3K_Akt_Signaling_and_Subsequent_ROS_Production_and_IKK/NF_κB_Activation_ L2 - https://www.cellphysiolbiochem.com/Articles/?DOI=10.33594/000000098 DB - PRIME DP - Unbound Medicine ER -