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Insect anionic septapeptides suppress DENV replication by activating antiviral cytokines and miRNAs in primary human monocytes.
Antiviral Res. 2019 08; 168:1-8.AR

Abstract

Dengue viruses (DENVs) have threatened 2/3 of the world population for decades. Thus, combating DENV infection with either antiviral therapy or protective vaccination is an urgent goal. In the present study, we investigated the anti-DENV activity of insect cell-derived anionic septapeptides from C6/36 mosquito cell cultures persistently infected with DENV. These molecules were previously shown to protect C6/36 and Vero cells against DENV infection. We found that treatment with these septapeptides strongly and rapidly downregulated the multiplication of DENV-1 16007, DENV-3 16562, and DENV-4 1036 but not that of DENV-2 16681 in primary human monocytes. This inhibitory effect was likely mediated through various routes including the increased production of antiviral cytokines (IFN-I), activation of mononuclear cell migration, and upregulation of the expression of antiviral miRNAs (has-miR-30e*, has-miR-133a, and has-miR-223) and inflammation-related miRNAs (has-miR-146a and has-miR-147). In conclusion, anionic septapeptides exerted anti-DENV activity in human monocytes through the upregulation of innate immune responses and the activation of several previously reported antiviral and inflammation-related miRNAs.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Limthongkul J
Department of Microbiology, Faculty of Science, Mahidol University, 272 Rama 6 Road, Bangkok, 10400, Thailand. Electronic address: jitra.kas@mahidol.ac.th.
Mapratiep N
Department of Microbiology, Faculty of Science, Mahidol University, 272 Rama 6 Road, Bangkok, 10400, Thailand. Electronic address: Nithipong_benhur@hotmail.com.
Apichirapokey S
Department of Microbiology, Faculty of Science, Mahidol University, 272 Rama 6 Road, Bangkok, 10400, Thailand. Electronic address: mind_apichirapokey@yahoo.com.
Suksatu A
Department of Microbiology, Faculty of Science, Mahidol University, 272 Rama 6 Road, Bangkok, 10400, Thailand. Electronic address: ampa.sus@mahidol.ac.th.
Midoeng P
Army Institute of Pathology, Phramongkutklao Hospital, Bangkok, Thailand. Electronic address: panuwat@windowslive.com.
Ubol S
Department of Microbiology, Faculty of Science, Mahidol University, 272 Rama 6 Road, Bangkok, 10400, Thailand. Electronic address: sukathida.ubo@mahidol.ac.th.

MeSH

AnimalsAntiviral AgentsCell MovementCells, CulturedChlorocebus aethiopsCulicidaeCytokinesDengueDengue VirusHumansImmunity, InnateMicroRNAsMonocytesPeptidesVero CellsVirus Replication

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31075349

Citation

Limthongkul, Jitra, et al. "Insect Anionic Septapeptides Suppress DENV Replication By Activating Antiviral Cytokines and miRNAs in Primary Human Monocytes." Antiviral Research, vol. 168, 2019, pp. 1-8.
Limthongkul J, Mapratiep N, Apichirapokey S, et al. Insect anionic septapeptides suppress DENV replication by activating antiviral cytokines and miRNAs in primary human monocytes. Antiviral Res. 2019;168:1-8.
Limthongkul, J., Mapratiep, N., Apichirapokey, S., Suksatu, A., Midoeng, P., & Ubol, S. (2019). Insect anionic septapeptides suppress DENV replication by activating antiviral cytokines and miRNAs in primary human monocytes. Antiviral Research, 168, 1-8. https://doi.org/10.1016/j.antiviral.2019.04.012
Limthongkul J, et al. Insect Anionic Septapeptides Suppress DENV Replication By Activating Antiviral Cytokines and miRNAs in Primary Human Monocytes. Antiviral Res. 2019;168:1-8. PubMed PMID: 31075349.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Insect anionic septapeptides suppress DENV replication by activating antiviral cytokines and miRNAs in primary human monocytes. AU - Limthongkul,Jitra, AU - Mapratiep,Nithipong, AU - Apichirapokey,Suttikarn, AU - Suksatu,Ampa, AU - Midoeng,Panuwat, AU - Ubol,Sukathida, Y1 - 2019/05/07/ PY - 2019/01/13/received PY - 2019/04/10/revised PY - 2019/04/24/accepted PY - 2019/5/11/pubmed PY - 2020/6/13/medline PY - 2019/5/11/entrez KW - Anti-DENV peptides KW - Innate responses KW - Insect-derived peptides KW - microRNA SP - 1 EP - 8 JF - Antiviral research JO - Antiviral Res VL - 168 N2 - Dengue viruses (DENVs) have threatened 2/3 of the world population for decades. Thus, combating DENV infection with either antiviral therapy or protective vaccination is an urgent goal. In the present study, we investigated the anti-DENV activity of insect cell-derived anionic septapeptides from C6/36 mosquito cell cultures persistently infected with DENV. These molecules were previously shown to protect C6/36 and Vero cells against DENV infection. We found that treatment with these septapeptides strongly and rapidly downregulated the multiplication of DENV-1 16007, DENV-3 16562, and DENV-4 1036 but not that of DENV-2 16681 in primary human monocytes. This inhibitory effect was likely mediated through various routes including the increased production of antiviral cytokines (IFN-I), activation of mononuclear cell migration, and upregulation of the expression of antiviral miRNAs (has-miR-30e*, has-miR-133a, and has-miR-223) and inflammation-related miRNAs (has-miR-146a and has-miR-147). In conclusion, anionic septapeptides exerted anti-DENV activity in human monocytes through the upregulation of innate immune responses and the activation of several previously reported antiviral and inflammation-related miRNAs. SN - 1872-9096 UR - https://www.unboundmedicine.com/medline/citation/31075349/Insect_anionic_septapeptides_suppress_DENV_replication_by_activating_antiviral_cytokines_and_miRNAs_in_primary_human_monocytes_ DB - PRIME DP - Unbound Medicine ER -