Tags

Type your tag names separated by a space and hit enter

Development and validation of an ultra-high performance liquid chromatography-high resolution mass spectrometry method for simultaneous quantification of cyanogenic glycosides and secoisolariciresinol diglucoside in flaxseed (Linum usitatissimum L.).
J Chromatogr A. 2019 Sep 13; 1601:214-223.JC

Abstract

An ultra performance liquid chromatography electrospray ionization high-resolution mass spectrometry (UPLC/ESI-HRMS) method was developed and validated for simultaneous quantification of cyanogenic glycosides (CGs), [linustatin (LIS) and neolinustatin (NLIS)], and the main lignan, secoisolariciresinol diglucoside (SDG) in Linoforce® (LF) [flaxseed (Linum usitatissimum L.) coated with two herbal extracts (Senna alexandrina mill and Frangula alnus)]. CGs and SDG were extracted from defatted ground LF by a new procedure consisting of an aqueous methanol ultrasound-assisted extraction followed by an aqueous alkaline ultrasound-assisted extraction of the residue. The combined extracted solutions were then hydrolyzed by 0.02 M NaOH to release SDG from its hydroxymethyl glutaryl ester-linked complex (SDG-HMG). After hydrolysis, the sample was acidified and analyzed directly, without the need of any additional clean-up steps, by UPLC/ESI-HRMS in positive mode. The identification of CGs and SDG was confirmed by the similar retention time and similar MS spectra to the corresponding authentic standards. The quantification was performed using the corresponding extracted ion chromatograms and amygdalin as internal standard. The overall method was validated in terms of linearity, stability, selectivity, precision and accuracy. The developed method was successfully applied to the quantification of CGs and SDG in LF and also in non-coated flaxseed. This is the first report on the simultaneous quantification of CGs and SDG in LF and flaxseed.

Authors+Show Affiliations

Chimie Analytique des Molécules Bioactives et Pharmacognosie, Institut Pluridisciplinaire Hubert Curien (UMR 7178 CNRS/UDS), 74 route du Rhin, 67400, Illkirch, France.Chimie Analytique des Molécules Bioactives et Pharmacognosie, Institut Pluridisciplinaire Hubert Curien (UMR 7178 CNRS/UDS), 74 route du Rhin, 67400, Illkirch, France.Laboratoire A. Vogel, 5A Rue Lavoisier, 68000, Colmar, France.Chimie Analytique des Molécules Bioactives et Pharmacognosie, Institut Pluridisciplinaire Hubert Curien (UMR 7178 CNRS/UDS), 74 route du Rhin, 67400, Illkirch, France. Electronic address: eric.marchioni@unistra.fr.

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

31076224

Citation

Zhao, Minjie, et al. "Development and Validation of an Ultra-high Performance Liquid Chromatography-high Resolution Mass Spectrometry Method for Simultaneous Quantification of Cyanogenic Glycosides and Secoisolariciresinol Diglucoside in Flaxseed (Linum Usitatissimum L.)." Journal of Chromatography. A, vol. 1601, 2019, pp. 214-223.
Zhao M, Bergaentzlé M, Flieller A, et al. Development and validation of an ultra-high performance liquid chromatography-high resolution mass spectrometry method for simultaneous quantification of cyanogenic glycosides and secoisolariciresinol diglucoside in flaxseed (Linum usitatissimum L.). J Chromatogr A. 2019;1601:214-223.
Zhao, M., Bergaentzlé, M., Flieller, A., & Marchioni, E. (2019). Development and validation of an ultra-high performance liquid chromatography-high resolution mass spectrometry method for simultaneous quantification of cyanogenic glycosides and secoisolariciresinol diglucoside in flaxseed (Linum usitatissimum L.). Journal of Chromatography. A, 1601, 214-223. https://doi.org/10.1016/j.chroma.2019.04.072
Zhao M, et al. Development and Validation of an Ultra-high Performance Liquid Chromatography-high Resolution Mass Spectrometry Method for Simultaneous Quantification of Cyanogenic Glycosides and Secoisolariciresinol Diglucoside in Flaxseed (Linum Usitatissimum L.). J Chromatogr A. 2019 Sep 13;1601:214-223. PubMed PMID: 31076224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of an ultra-high performance liquid chromatography-high resolution mass spectrometry method for simultaneous quantification of cyanogenic glycosides and secoisolariciresinol diglucoside in flaxseed (Linum usitatissimum L.). AU - Zhao,Minjie, AU - Bergaentzlé,Martine, AU - Flieller,Anne, AU - Marchioni,Eric, Y1 - 2019/04/29/ PY - 2018/12/17/received PY - 2019/04/26/revised PY - 2019/04/29/accepted PY - 2019/5/12/pubmed PY - 2019/7/25/medline PY - 2019/5/12/entrez KW - Cyanogenic glycosides KW - Flaxseed KW - Method development and validation KW - Secoisolariciresinol diglucoside KW - UPLC/ESI-HRMS KW - Ultrasound-assisted extraction SP - 214 EP - 223 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1601 N2 - An ultra performance liquid chromatography electrospray ionization high-resolution mass spectrometry (UPLC/ESI-HRMS) method was developed and validated for simultaneous quantification of cyanogenic glycosides (CGs), [linustatin (LIS) and neolinustatin (NLIS)], and the main lignan, secoisolariciresinol diglucoside (SDG) in Linoforce® (LF) [flaxseed (Linum usitatissimum L.) coated with two herbal extracts (Senna alexandrina mill and Frangula alnus)]. CGs and SDG were extracted from defatted ground LF by a new procedure consisting of an aqueous methanol ultrasound-assisted extraction followed by an aqueous alkaline ultrasound-assisted extraction of the residue. The combined extracted solutions were then hydrolyzed by 0.02 M NaOH to release SDG from its hydroxymethyl glutaryl ester-linked complex (SDG-HMG). After hydrolysis, the sample was acidified and analyzed directly, without the need of any additional clean-up steps, by UPLC/ESI-HRMS in positive mode. The identification of CGs and SDG was confirmed by the similar retention time and similar MS spectra to the corresponding authentic standards. The quantification was performed using the corresponding extracted ion chromatograms and amygdalin as internal standard. The overall method was validated in terms of linearity, stability, selectivity, precision and accuracy. The developed method was successfully applied to the quantification of CGs and SDG in LF and also in non-coated flaxseed. This is the first report on the simultaneous quantification of CGs and SDG in LF and flaxseed. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/31076224/Development_and_validation_of_an_ultra_high_performance_liquid_chromatography_high_resolution_mass_spectrometry_method_for_simultaneous_quantification_of_cyanogenic_glycosides_and_secoisolariciresinol_diglucoside_in_flaxseed__Linum_usitatissimum_L___ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(19)30475-3 DB - PRIME DP - Unbound Medicine ER -