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Polysaccharides from lichen Xanthoria parietina: 1,4/1,6-α-d-glucans and a highly branched galactomannan with macrophage stimulating activity via Dectin-2 activation.
Int J Biol Macromol. 2019 Aug 01; 134:921-935.IJ

Abstract

Hot-water soluble polysaccharides H-1-3 and H-2-1 were isolated from the thalli of the lichen Xanthoria parietina (L.) Th. Fr. and purified by ion exchange and gel permeation chromatography. Structure elucidation was mainly based on 2D-NMR and nano-ESI-Q-TOF MS/MS experiments. H-1-3 (13.7 kDa) was shown to be linear α-glucan with α-d-Glcp-(1 → [→[4)-α-d-Glcp-(1]2 → [6)-α-d-Glcp-(1]3 → 4)]n core backbone. The (1,4)- and (1,6)-α-d-Glcp linkages were in a 2:3 M ratio. H-2-1 (525 kDa) was characterized as a complex branched β-galacto-α-mannan with →[6)-α-d-Manp-(1 → [2,6)-α-d-Manp-(1]2 → [2)-α-d-Manp-(1]2→]n core units and main side chains of (1,3)-β-d-Galf linked at O-6 to →2)-α-d-Manp-(1→, together with minor terminal units of 1,4/1,6-α-D -Glcp units attached to the core chain at O-6 position and α-L-Rhap linked to Galf side chain at O-2 position (Manp: Galf: Glcp: Rhap linkage ratio = 9:3:2:1). H-2-1 exerted strong immunoactivity in vitro and activated murine RAW macrophages 264.7 towards significantly increased phagocytosis, TNF-α and IL-1β secretion. These effects are due to an interaction of the galactomannan with the transmembrane pattern-recognition protein Dectin-2 of the macrophages.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Rashid ZM
University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, Corrensstrasse 48, D-48149 Münster, Germany.
Mormann M
University of Münster, Institute for Hygiene, Robert-Koch-Strasse 41, D-48149 Münster, Germany.
Steckhan K
University of Münster, Institute for Hygiene, Robert-Koch-Strasse 41, D-48149 Münster, Germany.
Peters A
University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, Corrensstrasse 48, D-48149 Münster, Germany.
Esch S
University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, Corrensstrasse 48, D-48149 Münster, Germany.
Hensel A
University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, Corrensstrasse 48, D-48149 Münster, Germany. Electronic address: ahensel@uni-muenster.de.

MeSH

AnimalsGalactoseGlucansHydrolysisLectins, C-TypeLichensMacrophage ActivationMacrophagesMagnetic Resonance SpectroscopyMannansMiceMolecular WeightPhagocytosisPolysaccharidesRAW 264.7 CellsSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31078591

Citation

Rashid, Zalilawati Mat, et al. "Polysaccharides From Lichen Xanthoria Parietina: 1,4/1,6-α-d-glucans and a Highly Branched Galactomannan With Macrophage Stimulating Activity Via Dectin-2 Activation." International Journal of Biological Macromolecules, vol. 134, 2019, pp. 921-935.
Rashid ZM, Mormann M, Steckhan K, et al. Polysaccharides from lichen Xanthoria parietina: 1,4/1,6-α-d-glucans and a highly branched galactomannan with macrophage stimulating activity via Dectin-2 activation. Int J Biol Macromol. 2019;134:921-935.
Rashid, Z. M., Mormann, M., Steckhan, K., Peters, A., Esch, S., & Hensel, A. (2019). Polysaccharides from lichen Xanthoria parietina: 1,4/1,6-α-d-glucans and a highly branched galactomannan with macrophage stimulating activity via Dectin-2 activation. International Journal of Biological Macromolecules, 134, 921-935. https://doi.org/10.1016/j.ijbiomac.2019.05.056
Rashid ZM, et al. Polysaccharides From Lichen Xanthoria Parietina: 1,4/1,6-α-d-glucans and a Highly Branched Galactomannan With Macrophage Stimulating Activity Via Dectin-2 Activation. Int J Biol Macromol. 2019 Aug 1;134:921-935. PubMed PMID: 31078591.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polysaccharides from lichen Xanthoria parietina: 1,4/1,6-α-d-glucans and a highly branched galactomannan with macrophage stimulating activity via Dectin-2 activation. AU - Rashid,Zalilawati Mat, AU - Mormann,Michael, AU - Steckhan,Katja, AU - Peters,Alena, AU - Esch,Stefan, AU - Hensel,Andreas, Y1 - 2019/05/09/ PY - 2019/02/25/received PY - 2019/04/30/revised PY - 2019/05/08/accepted PY - 2019/5/13/pubmed PY - 2019/12/18/medline PY - 2019/5/13/entrez KW - Dectin-2 KW - Galactomannan KW - Glucan KW - Lichen KW - Mass spectroscopy KW - Phagocytosis KW - Polysaccharides KW - Silver adducts KW - Xanthoria parietina SP - 921 EP - 935 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 134 N2 - Hot-water soluble polysaccharides H-1-3 and H-2-1 were isolated from the thalli of the lichen Xanthoria parietina (L.) Th. Fr. and purified by ion exchange and gel permeation chromatography. Structure elucidation was mainly based on 2D-NMR and nano-ESI-Q-TOF MS/MS experiments. H-1-3 (13.7 kDa) was shown to be linear α-glucan with α-d-Glcp-(1 → [→[4)-α-d-Glcp-(1]2 → [6)-α-d-Glcp-(1]3 → 4)]n core backbone. The (1,4)- and (1,6)-α-d-Glcp linkages were in a 2:3 M ratio. H-2-1 (525 kDa) was characterized as a complex branched β-galacto-α-mannan with →[6)-α-d-Manp-(1 → [2,6)-α-d-Manp-(1]2 → [2)-α-d-Manp-(1]2→]n core units and main side chains of (1,3)-β-d-Galf linked at O-6 to →2)-α-d-Manp-(1→, together with minor terminal units of 1,4/1,6-α-D -Glcp units attached to the core chain at O-6 position and α-L-Rhap linked to Galf side chain at O-2 position (Manp: Galf: Glcp: Rhap linkage ratio = 9:3:2:1). H-2-1 exerted strong immunoactivity in vitro and activated murine RAW macrophages 264.7 towards significantly increased phagocytosis, TNF-α and IL-1β secretion. These effects are due to an interaction of the galactomannan with the transmembrane pattern-recognition protein Dectin-2 of the macrophages. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/31078591/Polysaccharides_from_lichen_Xanthoria_parietina:_14/16_α_d_glucans_and_a_highly_branched_galactomannan_with_macrophage_stimulating_activity_via_Dectin_2_activation_ DB - PRIME DP - Unbound Medicine ER -