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Tillering and small grain 1 dominates the tryptophan aminotransferase family required for local auxin biosynthesis in rice.
J Integr Plant Biol. 2020 May; 62(5):581-600.JI

Abstract

Auxin is a crucial phytohormone, controlling multiple aspects of plant growth and responses to the changing environment. However, the role of local auxin biosynthesis in specific developmental programs remains unknown in crops. This study characterized the rice tillering and small grain 1 (tsg1) mutant, which has more tillers but a smaller panicle and grain size resulting from a reduction in endogenous auxin. TSG1 encodes a tryptophan aminotransferase that is allelic to the FISH BONE (FIB) gene. The tsg1 mutant showed hypersensitivity to indole-3-acetic acid and the competitive inhibitor of aminotransferase, L-kynurenine. TSG1 knockout resulted in an increased tiller number but reduction in grain number and size, and decrease in height. Meanwhile, deletion of the TSG1 homologs OsTAR1, OsTARL1, and OsTARL2 caused no obvious changes, although the phenotype of the TSG1/OsTAR1 double mutant was intensified and infertile, suggesting gene redundancy in the rice tryptophan aminotransferase family. Interestingly, TSG1 and OsTAR1, but not OsTARL1 and OsTARL2, displayed marked aminotransferase activity. Meanwhile, subcellular localization was identified as the endoplasmic reticulum, while phylogenetic analysis revealed functional divergence of TSG1 and OsTAR1 from OsTARL1 and OsTARL2. These findings suggest that TSG1 dominates the tryptophan aminotransferase family, playing a prominent role in local auxin biosynthesis in rice.

Authors+Show Affiliations

National Key Laboratory of Plant Molecular Genetics, CAS Centre for Excellence in Molecular Plant Sciences and Collaborative Innovation Center of Genetics & Development, Shanghai Institute of Plant Physiology & Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.National Key Laboratory of Plant Molecular Genetics, CAS Centre for Excellence in Molecular Plant Sciences and Collaborative Innovation Center of Genetics & Development, Shanghai Institute of Plant Physiology & Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China. University of the Chinese Academy of Sciences, Beijing, 100049, China.National Key Laboratory of Plant Molecular Genetics, CAS Centre for Excellence in Molecular Plant Sciences and Collaborative Innovation Center of Genetics & Development, Shanghai Institute of Plant Physiology & Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.National Key Laboratory of Plant Molecular Genetics, CAS Centre for Excellence in Molecular Plant Sciences and Collaborative Innovation Center of Genetics & Development, Shanghai Institute of Plant Physiology & Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.National Key Laboratory of Plant Molecular Genetics, CAS Centre for Excellence in Molecular Plant Sciences and Collaborative Innovation Center of Genetics & Development, Shanghai Institute of Plant Physiology & Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.National Key Laboratory of Plant Molecular Genetics, CAS Centre for Excellence in Molecular Plant Sciences and Collaborative Innovation Center of Genetics & Development, Shanghai Institute of Plant Physiology & Ecology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China. University of the Chinese Academy of Sciences, Beijing, 100049, China. School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31081210

Citation

Guo, Tao, et al. "Tillering and Small Grain 1 Dominates the Tryptophan Aminotransferase Family Required for Local Auxin Biosynthesis in Rice." Journal of Integrative Plant Biology, vol. 62, no. 5, 2020, pp. 581-600.
Guo T, Chen K, Dong NQ, et al. Tillering and small grain 1 dominates the tryptophan aminotransferase family required for local auxin biosynthesis in rice. J Integr Plant Biol. 2020;62(5):581-600.
Guo, T., Chen, K., Dong, N. Q., Ye, W. W., Shan, J. X., & Lin, H. X. (2020). Tillering and small grain 1 dominates the tryptophan aminotransferase family required for local auxin biosynthesis in rice. Journal of Integrative Plant Biology, 62(5), 581-600. https://doi.org/10.1111/jipb.12820
Guo T, et al. Tillering and Small Grain 1 Dominates the Tryptophan Aminotransferase Family Required for Local Auxin Biosynthesis in Rice. J Integr Plant Biol. 2020;62(5):581-600. PubMed PMID: 31081210.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tillering and small grain 1 dominates the tryptophan aminotransferase family required for local auxin biosynthesis in rice. AU - Guo,Tao, AU - Chen,Ke, AU - Dong,Nai-Qian, AU - Ye,Wang-Wei, AU - Shan,Jun-Xiang, AU - Lin,Hong-Xuan, Y1 - 2019/09/09/ PY - 2019/03/06/received PY - 2019/04/26/accepted PY - 2019/5/14/pubmed PY - 2021/1/26/medline PY - 2019/5/14/entrez SP - 581 EP - 600 JF - Journal of integrative plant biology JO - J Integr Plant Biol VL - 62 IS - 5 N2 - Auxin is a crucial phytohormone, controlling multiple aspects of plant growth and responses to the changing environment. However, the role of local auxin biosynthesis in specific developmental programs remains unknown in crops. This study characterized the rice tillering and small grain 1 (tsg1) mutant, which has more tillers but a smaller panicle and grain size resulting from a reduction in endogenous auxin. TSG1 encodes a tryptophan aminotransferase that is allelic to the FISH BONE (FIB) gene. The tsg1 mutant showed hypersensitivity to indole-3-acetic acid and the competitive inhibitor of aminotransferase, L-kynurenine. TSG1 knockout resulted in an increased tiller number but reduction in grain number and size, and decrease in height. Meanwhile, deletion of the TSG1 homologs OsTAR1, OsTARL1, and OsTARL2 caused no obvious changes, although the phenotype of the TSG1/OsTAR1 double mutant was intensified and infertile, suggesting gene redundancy in the rice tryptophan aminotransferase family. Interestingly, TSG1 and OsTAR1, but not OsTARL1 and OsTARL2, displayed marked aminotransferase activity. Meanwhile, subcellular localization was identified as the endoplasmic reticulum, while phylogenetic analysis revealed functional divergence of TSG1 and OsTAR1 from OsTARL1 and OsTARL2. These findings suggest that TSG1 dominates the tryptophan aminotransferase family, playing a prominent role in local auxin biosynthesis in rice. SN - 1744-7909 UR - https://www.unboundmedicine.com/medline/citation/31081210/Tillering_and_small_grain_1_dominates_the_tryptophan_aminotransferase_family_required_for_local_auxin_biosynthesis_in_rice_ L2 - https://doi.org/10.1111/jipb.12820 DB - PRIME DP - Unbound Medicine ER -