Tags

Type your tag names separated by a space and hit enter

Offspring analysis using two cleaved amplified polymorphic sequence (CAPS) markers reveals amphithallism in the edible mushroom Agaricus sinodeliciosus.
Mycologia. 2019 May-Jun; 111(3):384-394.M

Abstract

Agaricus sinodeliciosus is an edible wild mushroom known in northwest China. It belongs to Agaricus section Bivelares that includes several popular cultivated species, such as A. bisporus, the button mushroom. The life cycle of the latter species has been described as amphithallic because both homokaryotic (n) and heterokaryotic (n+n) spores are produced that lead to heterothallic and pseudohomothallic life cycles, respectively. The type of life cycle can impact population structures and breeding strategies. The main objective of this study was to identify the different categories of spores produced by A. sinodeliciosus. Using either a morphological approach based on the number of sterigmata per basidium or a genetic approach based on the genotypes of the progeny at two loci, the proportion of heterokaryotic spores was estimated at 6% and 15%, respectively. Two codominant markers were chosen from the mitochondrial intermediate peptidase gene (MIP) and the nuc rDNA internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) region. Genotypic analysis and mating tests confirmed that in A. sinodeliciosus, MIP is centromere-linked and tightly linked to the mating type locus as in A. bisporus and A. subrufescens. We conclude that A. sinodeliciosus has a unifactorial system of sexual incompatibility and an amphithallic life cycle that is predominantly heterothallic, and that its pseudohomothallism follows a nonrandom model with nonsister postmeiotic nuclei paired in the same spore, which give rise to a potentially fertile heterokaryon. This method of using two informative markers is reliable not only in selecting the homokaryotic offspring but also in classifying the homokaryons in two breeding stocks according to their mating type alleles.

Authors+Show Affiliations

a State Key Laboratory of Mycology , Institute of Microbiology, Chinese Academy of Sciences , Chaoyang District , Beijing 100101 , People's Republic of China. b College of Life Sciences , University of Chinese Academy of Sciences , Huairou District , Beijing 100408 , People's Republic of China.a State Key Laboratory of Mycology , Institute of Microbiology, Chinese Academy of Sciences , Chaoyang District , Beijing 100101 , People's Republic of China.c Institute National de la Recherche Agronomique (INRA) , Mycologie et sécurité des aliments, CS 20032, 33883 Villenave d'Ornon Cedex , France.a State Key Laboratory of Mycology , Institute of Microbiology, Chinese Academy of Sciences , Chaoyang District , Beijing 100101 , People's Republic of China. b College of Life Sciences , University of Chinese Academy of Sciences , Huairou District , Beijing 100408 , People's Republic of China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31084454

Citation

Ling, Zhi-Lin, et al. "Offspring Analysis Using Two Cleaved Amplified Polymorphic Sequence (CAPS) Markers Reveals Amphithallism in the Edible Mushroom Agaricus Sinodeliciosus." Mycologia, vol. 111, no. 3, 2019, pp. 384-394.
Ling ZL, Wang HJ, Callac P, et al. Offspring analysis using two cleaved amplified polymorphic sequence (CAPS) markers reveals amphithallism in the edible mushroom Agaricus sinodeliciosus. Mycologia. 2019;111(3):384-394.
Ling, Z. L., Wang, H. J., Callac, P., & Zhao, R. L. (2019). Offspring analysis using two cleaved amplified polymorphic sequence (CAPS) markers reveals amphithallism in the edible mushroom Agaricus sinodeliciosus. Mycologia, 111(3), 384-394. https://doi.org/10.1080/00275514.2019.1599248
Ling ZL, et al. Offspring Analysis Using Two Cleaved Amplified Polymorphic Sequence (CAPS) Markers Reveals Amphithallism in the Edible Mushroom Agaricus Sinodeliciosus. Mycologia. 2019 May-Jun;111(3):384-394. PubMed PMID: 31084454.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Offspring analysis using two cleaved amplified polymorphic sequence (CAPS) markers reveals amphithallism in the edible mushroom Agaricus sinodeliciosus. AU - Ling,Zhi-Lin, AU - Wang,Hui-Jun, AU - Callac,Philippe, AU - Zhao,Rui-Lin, Y1 - 2019/05/14/ PY - 2019/5/16/pubmed PY - 2020/4/15/medline PY - 2019/5/16/entrez KW - Life cycle KW - meiosis KW - mitochondrial intermediate peptidase gene KW - recombination SP - 384 EP - 394 JF - Mycologia JO - Mycologia VL - 111 IS - 3 N2 - Agaricus sinodeliciosus is an edible wild mushroom known in northwest China. It belongs to Agaricus section Bivelares that includes several popular cultivated species, such as A. bisporus, the button mushroom. The life cycle of the latter species has been described as amphithallic because both homokaryotic (n) and heterokaryotic (n+n) spores are produced that lead to heterothallic and pseudohomothallic life cycles, respectively. The type of life cycle can impact population structures and breeding strategies. The main objective of this study was to identify the different categories of spores produced by A. sinodeliciosus. Using either a morphological approach based on the number of sterigmata per basidium or a genetic approach based on the genotypes of the progeny at two loci, the proportion of heterokaryotic spores was estimated at 6% and 15%, respectively. Two codominant markers were chosen from the mitochondrial intermediate peptidase gene (MIP) and the nuc rDNA internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) region. Genotypic analysis and mating tests confirmed that in A. sinodeliciosus, MIP is centromere-linked and tightly linked to the mating type locus as in A. bisporus and A. subrufescens. We conclude that A. sinodeliciosus has a unifactorial system of sexual incompatibility and an amphithallic life cycle that is predominantly heterothallic, and that its pseudohomothallism follows a nonrandom model with nonsister postmeiotic nuclei paired in the same spore, which give rise to a potentially fertile heterokaryon. This method of using two informative markers is reliable not only in selecting the homokaryotic offspring but also in classifying the homokaryons in two breeding stocks according to their mating type alleles. SN - 1557-2536 UR - https://www.unboundmedicine.com/medline/citation/31084454/Offspring_analysis_using_two_cleaved_amplified_polymorphic_sequence__CAPS__markers_reveals_amphithallism_in_the_edible_mushroom_Agaricus_sinodeliciosus_ L2 - http://www.tandfonline.com/doi/full/10.1080/00275514.2019.1599248 DB - PRIME DP - Unbound Medicine ER -