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The host basal transcription factor IIA subunits coordinate for facilitating infection of TALEs-carrying bacterial pathogens in rice.
Plant Sci. 2019 Jul; 284:48-56.PS

Abstract

Many plant-pathogenic Xanthomonas rely largely on secreting virulence transcription activator-like effectors (TALEs) proteins into plant nucleus to activate host susceptibility genes to cause disease, the process is dependent on pathogen TALEs association with host plants basal transcription factor IIA small subunit TFIIAγ. TFIIAγ together with large subunit TFIIAαβ constitute as a key component of RNA polymerase II complex for transcriptome initiation. However, whether TFIIAαβ coordinates or competes with pathogen TALEs for interaction with TFIIAγ to activate transcript of TALEs-targeting genes is unclear. Here, we showed that TALE-carrying bacterial pathogens Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), the causal agent for bacterial leaf blight and bacterial leaf streak in rice, using their major virulence TALEs to physically associate with N-terminal of OsTFIIAγ5. OsTFIIAα and OsTFIIAβ which are post-translationally mature proteins of OsTFIIAαβ separately bound to N- and C-terminal of OsTFIIAγ5. OsTFIIAα coordinated with TALEs for binding with OsTFIIAγ5 to upregulate rice susceptibility genes to cause disease. Conversely, suppression of OsTFIIAαβ attenuated TALEs-targeting genes transcription, thus improved broad-spectrum disease resistance of rice to Xoo and Xoc. These results provide an applicable strategy for improving resistance to TALE-carrying pathogens in rice by appropriate suppression of plant basal transcription factors expression.

Authors+Show Affiliations

National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: myuan@mail.hzau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31084878

Citation

Hui, Shugang, et al. "The Host Basal Transcription Factor IIA Subunits Coordinate for Facilitating Infection of TALEs-carrying Bacterial Pathogens in Rice." Plant Science : an International Journal of Experimental Plant Biology, vol. 284, 2019, pp. 48-56.
Hui S, Liu H, Zhang M, et al. The host basal transcription factor IIA subunits coordinate for facilitating infection of TALEs-carrying bacterial pathogens in rice. Plant Sci. 2019;284:48-56.
Hui, S., Liu, H., Zhang, M., Chen, D., Li, Q., Tian, J., Xiao, J., Li, X., Wang, S., & Yuan, M. (2019). The host basal transcription factor IIA subunits coordinate for facilitating infection of TALEs-carrying bacterial pathogens in rice. Plant Science : an International Journal of Experimental Plant Biology, 284, 48-56. https://doi.org/10.1016/j.plantsci.2019.04.004
Hui S, et al. The Host Basal Transcription Factor IIA Subunits Coordinate for Facilitating Infection of TALEs-carrying Bacterial Pathogens in Rice. Plant Sci. 2019;284:48-56. PubMed PMID: 31084878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The host basal transcription factor IIA subunits coordinate for facilitating infection of TALEs-carrying bacterial pathogens in rice. AU - Hui,Shugang, AU - Liu,Huiling, AU - Zhang,Meng, AU - Chen,Dan, AU - Li,Qingqing, AU - Tian,Jingjing, AU - Xiao,Jinghua, AU - Li,Xianghua, AU - Wang,Shiping, AU - Yuan,Meng, Y1 - 2019/04/06/ PY - 2019/01/07/received PY - 2019/03/13/revised PY - 2019/04/03/accepted PY - 2019/5/16/entrez PY - 2019/5/16/pubmed PY - 2019/6/7/medline KW - Rice KW - TFIIA KW - Transcription activator-like effector KW - Xanthomonas SP - 48 EP - 56 JF - Plant science : an international journal of experimental plant biology JO - Plant Sci VL - 284 N2 - Many plant-pathogenic Xanthomonas rely largely on secreting virulence transcription activator-like effectors (TALEs) proteins into plant nucleus to activate host susceptibility genes to cause disease, the process is dependent on pathogen TALEs association with host plants basal transcription factor IIA small subunit TFIIAγ. TFIIAγ together with large subunit TFIIAαβ constitute as a key component of RNA polymerase II complex for transcriptome initiation. However, whether TFIIAαβ coordinates or competes with pathogen TALEs for interaction with TFIIAγ to activate transcript of TALEs-targeting genes is unclear. Here, we showed that TALE-carrying bacterial pathogens Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), the causal agent for bacterial leaf blight and bacterial leaf streak in rice, using their major virulence TALEs to physically associate with N-terminal of OsTFIIAγ5. OsTFIIAα and OsTFIIAβ which are post-translationally mature proteins of OsTFIIAαβ separately bound to N- and C-terminal of OsTFIIAγ5. OsTFIIAα coordinated with TALEs for binding with OsTFIIAγ5 to upregulate rice susceptibility genes to cause disease. Conversely, suppression of OsTFIIAαβ attenuated TALEs-targeting genes transcription, thus improved broad-spectrum disease resistance of rice to Xoo and Xoc. These results provide an applicable strategy for improving resistance to TALE-carrying pathogens in rice by appropriate suppression of plant basal transcription factors expression. SN - 1873-2259 UR - https://www.unboundmedicine.com/medline/citation/31084878/The_host_basal_transcription_factor_IIA_subunits_coordinate_for_facilitating_infection_of_TALEs_carrying_bacterial_pathogens_in_rice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-9452(19)30028-7 DB - PRIME DP - Unbound Medicine ER -