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Bromelain-loaded chitosan nanofibers prepared by electrospinning method for burn wound healing in animal models.
Life Sci 2019; 229:57-66LS

Abstract

Bromelain is a mixture of proteolytic enzymes present in all tissues of pineapple (Ananas comosus). It is known as an efficient debriding agent in burn treatment. In this study, the efficiency of bromelain-loaded chitosan nanofibers for burn wounds repair was investigated in animal model. Chitosan nanofibers containing bromelain (2% and 4% w/v) were prepared by electrospinning method. The physicochemical characteristics of the synthetized nanofibers were evaluated. The release profile and activity of bromelain loaded in nanofibers were also assayed. Cytotoxicity test was carried out using Alamar blue. The burn healing effect of chitosan-2% w/v bromelain nanofiber was studied in the induced burn wounds in rats for 21 days. The efficacy of treatment was assessed by reduction of burn wound area and histological characteristics at different times. Chitosan-2% w/v bromelain showed the better physicochemical properties and release profile as well as low cytotoxicity than chitosan-4% w/v bromelain. The results also indicated that chitosan-2% w/v bromelain nanofiber was more efficient to heal burn skin compared to chitosan nanofiber alone in the animal model tested. The present study concludes that chitosan-2% w/v bromelain nanofiber possesses great wound healing activity and could be considered as an effective natural topical burn wound healing treatment.

Authors+Show Affiliations

Scool of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran; Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: MovaffaghJ@mums.ac.ir.Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: hashemim@mums.ac.ir.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31085247

Citation

Bayat, Samaneh, et al. "Bromelain-loaded Chitosan Nanofibers Prepared By Electrospinning Method for Burn Wound Healing in Animal Models." Life Sciences, vol. 229, 2019, pp. 57-66.
Bayat S, Amiri N, Pishavar E, et al. Bromelain-loaded chitosan nanofibers prepared by electrospinning method for burn wound healing in animal models. Life Sci. 2019;229:57-66.
Bayat, S., Amiri, N., Pishavar, E., Kalalinia, F., Movaffagh, J., & Hashemi, M. (2019). Bromelain-loaded chitosan nanofibers prepared by electrospinning method for burn wound healing in animal models. Life Sciences, 229, pp. 57-66. doi:10.1016/j.lfs.2019.05.028.
Bayat S, et al. Bromelain-loaded Chitosan Nanofibers Prepared By Electrospinning Method for Burn Wound Healing in Animal Models. Life Sci. 2019 Jul 15;229:57-66. PubMed PMID: 31085247.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bromelain-loaded chitosan nanofibers prepared by electrospinning method for burn wound healing in animal models. AU - Bayat,Samaneh, AU - Amiri,Nafise, AU - Pishavar,Elham, AU - Kalalinia,Fatemeh, AU - Movaffagh,Jebrail, AU - Hashemi,Maryam, Y1 - 2019/05/11/ PY - 2019/03/04/received PY - 2019/04/27/revised PY - 2019/05/10/accepted PY - 2019/5/16/pubmed PY - 2019/7/6/medline PY - 2019/5/16/entrez KW - Bromelain KW - Burn wound healing KW - Chitosan KW - Electrospinning KW - Nanofiber SP - 57 EP - 66 JF - Life sciences JO - Life Sci. VL - 229 N2 - Bromelain is a mixture of proteolytic enzymes present in all tissues of pineapple (Ananas comosus). It is known as an efficient debriding agent in burn treatment. In this study, the efficiency of bromelain-loaded chitosan nanofibers for burn wounds repair was investigated in animal model. Chitosan nanofibers containing bromelain (2% and 4% w/v) were prepared by electrospinning method. The physicochemical characteristics of the synthetized nanofibers were evaluated. The release profile and activity of bromelain loaded in nanofibers were also assayed. Cytotoxicity test was carried out using Alamar blue. The burn healing effect of chitosan-2% w/v bromelain nanofiber was studied in the induced burn wounds in rats for 21 days. The efficacy of treatment was assessed by reduction of burn wound area and histological characteristics at different times. Chitosan-2% w/v bromelain showed the better physicochemical properties and release profile as well as low cytotoxicity than chitosan-4% w/v bromelain. The results also indicated that chitosan-2% w/v bromelain nanofiber was more efficient to heal burn skin compared to chitosan nanofiber alone in the animal model tested. The present study concludes that chitosan-2% w/v bromelain nanofiber possesses great wound healing activity and could be considered as an effective natural topical burn wound healing treatment. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/31085247/Bromelain-loaded_chitosan_nanofibers_prepared_by_electrospinning_method_for_burn_wound_healing_in_animal_models L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(19)30374-1 DB - PRIME DP - Unbound Medicine ER -