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Effects of dietary b-glucan, mannan oligosaccharide, Lactobacillus plantarum and their combinations on growth performance, immunity and immune related gene expression of Caspian trout, Salmo trutta caspius (Kessler, 1877).
Fish Shellfish Immunol. 2019 Aug; 91:202-208.FS

Abstract

This study investigated the effect of individual and combination of dietary pre- and probiotics (β-glucan, 3 mg/g; mannan oligosaccharide (MOS), 4 mg/g; and Lactobacillus plantarum; 108 CFU/mg diet) on growth performance, blood immune parameters, expression of immune related genes, and intestinal microbial of Caspian trout (Salmo trutta caspius). On the basis of feeding with immunostimulant diets, the fish were assigned into eight groups denoted as: control (basal diet), bβ (basal diet + β-glucan), bM (basal diet + MOS), bLp (basal diet + L. plantarum), bβLp (basal diet + β-glucan + L. plantarum), bMLp (basal diet + MOS + L. plantarum), bMβ (basal diet + MOS + β-glucan), and bMβLp (basal diet + MOS + β-glucan + L. plantarum). All of the immunostimulant diets, in general, reduced feed intake (FI) and food conversion ratio (FCR) and increased WG, PER, and final weight. Condition factor (CF) demonstrated the lowest level in the experimental group received bMβLp. Total lipid increased in the fish received the additives, especially bM and bMβ. Ash content demonstrated significant increase in the fish fed on bβ and bMβLp, whereas moisture content was reduced in the group fed with L. plantarum-supplemented diet. All immunostimulant diets enhanced the activity and levels of lysozyme, Immunoglobulin M (IgM), and serum alternative complement activity (ACH50); the highest value for these indices was observed in the groups fed with bMβ, bMβLp, and bβLp. bMβ-treated fish group displayed the highest cortisol and glucose levels. bM diet induced the highest mRNA transcription of TNF-α1 in head kidney, whereas bLp, bMβ, and bMβLp showed no effect. IL1β exhibited the greatest up-regulation, about 8.75 fold change, in response to the diet supplemented only with β-glucan. bβLp and bβ significantly enhanced the relative IL-8 mRNA expression in the head kidney (about 2.75 and 1.9 folds, respectively), yet in response to bMβLp treatment it showed a decrease of about 5.7 times lower than the control group. In addition, intestinal population of L. plantarum showed the highest loads in the groups fed on the diets which were treated with the probiotic. Taken together, combinational use of these immunostimulants enhanced humoral innate immune system, whereas their individual and combinational application could increase and decrease the transcription of inflammation-related genes, respectively.

Authors+Show Affiliations

Department of Aquaculture, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares, University, Mazandaran, Noor, P.O. Box 64414-356, Iran.Department of Aquaculture, Faculty of Natural Resources and Marine Sciences, Tarbiat Modares, University, Mazandaran, Noor, P.O. Box 64414-356, Iran. Electronic address: aabedian@modares.ac.ir.Department of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.Cold Water Fishes Research Center (CFRC), Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Eduction and Extension Organization (AREEO), Dohezar, Tonekabon, Mazandaran, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31085328

Citation

Jami, Mohammad Javad, et al. "Effects of Dietary B-glucan, Mannan Oligosaccharide, Lactobacillus Plantarum and Their Combinations On Growth Performance, Immunity and Immune Related Gene Expression of Caspian Trout, Salmo Trutta Caspius (Kessler, 1877)." Fish & Shellfish Immunology, vol. 91, 2019, pp. 202-208.
Jami MJ, Abedian Kenari A, Paknejad H, et al. Effects of dietary b-glucan, mannan oligosaccharide, Lactobacillus plantarum and their combinations on growth performance, immunity and immune related gene expression of Caspian trout, Salmo trutta caspius (Kessler, 1877). Fish Shellfish Immunol. 2019;91:202-208.
Jami, M. J., Abedian Kenari, A., Paknejad, H., & Mohseni, M. (2019). Effects of dietary b-glucan, mannan oligosaccharide, Lactobacillus plantarum and their combinations on growth performance, immunity and immune related gene expression of Caspian trout, Salmo trutta caspius (Kessler, 1877). Fish & Shellfish Immunology, 91, 202-208. https://doi.org/10.1016/j.fsi.2019.05.024
Jami MJ, et al. Effects of Dietary B-glucan, Mannan Oligosaccharide, Lactobacillus Plantarum and Their Combinations On Growth Performance, Immunity and Immune Related Gene Expression of Caspian Trout, Salmo Trutta Caspius (Kessler, 1877). Fish Shellfish Immunol. 2019;91:202-208. PubMed PMID: 31085328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of dietary b-glucan, mannan oligosaccharide, Lactobacillus plantarum and their combinations on growth performance, immunity and immune related gene expression of Caspian trout, Salmo trutta caspius (Kessler, 1877). AU - Jami,Mohammad Javad, AU - Abedian Kenari,Abdolmohammad, AU - Paknejad,Hamed, AU - Mohseni,Mahmoud, Y1 - 2019/05/11/ PY - 2018/12/27/received PY - 2019/05/05/revised PY - 2019/05/10/accepted PY - 2019/5/16/pubmed PY - 2019/10/30/medline PY - 2019/5/16/entrez KW - Caspian trout KW - Immunity KW - Lactobacillus plantarum KW - Mannan oligosaccharide KW - Synbiotic KW - β-Glucan SP - 202 EP - 208 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 91 N2 - This study investigated the effect of individual and combination of dietary pre- and probiotics (β-glucan, 3 mg/g; mannan oligosaccharide (MOS), 4 mg/g; and Lactobacillus plantarum; 108 CFU/mg diet) on growth performance, blood immune parameters, expression of immune related genes, and intestinal microbial of Caspian trout (Salmo trutta caspius). On the basis of feeding with immunostimulant diets, the fish were assigned into eight groups denoted as: control (basal diet), bβ (basal diet + β-glucan), bM (basal diet + MOS), bLp (basal diet + L. plantarum), bβLp (basal diet + β-glucan + L. plantarum), bMLp (basal diet + MOS + L. plantarum), bMβ (basal diet + MOS + β-glucan), and bMβLp (basal diet + MOS + β-glucan + L. plantarum). All of the immunostimulant diets, in general, reduced feed intake (FI) and food conversion ratio (FCR) and increased WG, PER, and final weight. Condition factor (CF) demonstrated the lowest level in the experimental group received bMβLp. Total lipid increased in the fish received the additives, especially bM and bMβ. Ash content demonstrated significant increase in the fish fed on bβ and bMβLp, whereas moisture content was reduced in the group fed with L. plantarum-supplemented diet. All immunostimulant diets enhanced the activity and levels of lysozyme, Immunoglobulin M (IgM), and serum alternative complement activity (ACH50); the highest value for these indices was observed in the groups fed with bMβ, bMβLp, and bβLp. bMβ-treated fish group displayed the highest cortisol and glucose levels. bM diet induced the highest mRNA transcription of TNF-α1 in head kidney, whereas bLp, bMβ, and bMβLp showed no effect. IL1β exhibited the greatest up-regulation, about 8.75 fold change, in response to the diet supplemented only with β-glucan. bβLp and bβ significantly enhanced the relative IL-8 mRNA expression in the head kidney (about 2.75 and 1.9 folds, respectively), yet in response to bMβLp treatment it showed a decrease of about 5.7 times lower than the control group. In addition, intestinal population of L. plantarum showed the highest loads in the groups fed on the diets which were treated with the probiotic. Taken together, combinational use of these immunostimulants enhanced humoral innate immune system, whereas their individual and combinational application could increase and decrease the transcription of inflammation-related genes, respectively. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/31085328/Effects_of_dietary_b_glucan_mannan_oligosaccharide_Lactobacillus_plantarum_and_their_combinations_on_growth_performance_immunity_and_immune_related_gene_expression_of_Caspian_trout_Salmo_trutta_caspius__Kessler_1877__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(19)30580-7 DB - PRIME DP - Unbound Medicine ER -