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Phenotypic and genotypic characterization of enterotoxigenic Escherichia coli isolated from diarrheic calves in Argentina.
Open Vet J 2019; 9(1):65-73OV

Abstract

Enterotoxigenic Escherichia coli (ETEC) is the most common and global cause of neonatal calf diarrhea, but there is a little information regarding calf ETEC strains in Argentina. In this study, five ETEC isolates from diarrheic dairy calves (2-10 d old) from Buenos Aires and Cordoba, Argentina were characterized on the basis of virulence gene (VG) pattern, O:H serotyping, hemolytic phenotype, phylogenetic group affiliation, antimicrobial (AM) resistance profile, and presence of integron class 1 and 2. The five isolates were examined by polymerase chain reaction (PCR) for the presence of 18 bovine VGs and showed the following genotypes: F5+/F41+/sta + (D242), F5+/sta + (D158), F5+/sta + (D157), F5+ (D151-9), and F5+/iucD + (D151-5). These VGs confer pathogenic potential and most of them are associated with the ETEC pathotype. The five isolates showed a non-hemolytic phenotype, belonged to five different serotypes: O101:H-, O141:H-, O60:H-, ONT:H10, and ONT:H-, and were assigned to the phylogenetic group A by the quadruplex Clermont PCR method. The AM resistance of the three isolates D242, D157, and D151-5 was determined by agar disk diffusion method for 24 AMs and they exhibited a multi-resistance phenotype (resistance to four different AM classes: Cephalosporins, Penicillins, Macrolides, and Ansamycins). In addition, class 1 integrons were found in the isolate D151-5 containing the dfrA17-aadA5 gene cassette and in the bovine ETEC reference strain FV10191 containing the dfrA1-aadA1 gene cassette. The present study revealed for the first time the occurrence of multi-resistant ETEC associated with neonatal diarrhea in dairy calves in Argentina. This finding may be used for diagnostic and therapeutic purposes.

Authors+Show Affiliations

Departamento de Producción Animal, Laboratorio de Bacteriología, EEA INTA Balcarce, Balcarce, Argentina.Laboratorio de Inmunoquímica y Biotecnología, Centro de Investigación Veterinaria de Tandil, Facultad de Ciencias Veterinarias, UNCPBA, Tandil, Argentina.Laboratorio de Inmunoquímica y Biotecnología, Centro de Investigación Veterinaria de Tandil, Facultad de Ciencias Veterinarias, UNCPBA, Tandil, Argentina.Departamento de Producción Animal, Laboratorio de Bacteriología, EEA INTA Balcarce, Balcarce, Argentina.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31086769

Citation

González Pasayo, Ramón A., et al. "Phenotypic and Genotypic Characterization of Enterotoxigenic Escherichia Coli Isolated From Diarrheic Calves in Argentina." Open Veterinary Journal, vol. 9, no. 1, 2019, pp. 65-73.
González Pasayo RA, Sanz ME, Padola NL, et al. Phenotypic and genotypic characterization of enterotoxigenic Escherichia coli isolated from diarrheic calves in Argentina. Open Vet J. 2019;9(1):65-73.
González Pasayo, R. A., Sanz, M. E., Padola, N. L., & Moreira, A. R. (2019). Phenotypic and genotypic characterization of enterotoxigenic Escherichia coli isolated from diarrheic calves in Argentina. Open Veterinary Journal, 9(1), pp. 65-73. doi:10.4314/ovj.v9i1.12.
González Pasayo RA, et al. Phenotypic and Genotypic Characterization of Enterotoxigenic Escherichia Coli Isolated From Diarrheic Calves in Argentina. Open Vet J. 2019;9(1):65-73. PubMed PMID: 31086769.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic and genotypic characterization of enterotoxigenic Escherichia coli isolated from diarrheic calves in Argentina. AU - González Pasayo,Ramón A, AU - Sanz,Marcelo E, AU - Padola,Nora L, AU - Moreira,Ana R, Y1 - 2019/03/15/ PY - 2018/07/20/received PY - 2019/02/14/accepted PY - 2019/5/16/entrez PY - 2019/5/16/pubmed PY - 2019/5/16/medline KW - Antimicrobial resistance KW - Dairy cattle KW - Escherichia coli KW - Neonatal diarrhea KW - Virulence gene SP - 65 EP - 73 JF - Open veterinary journal JO - Open Vet J VL - 9 IS - 1 N2 - Enterotoxigenic Escherichia coli (ETEC) is the most common and global cause of neonatal calf diarrhea, but there is a little information regarding calf ETEC strains in Argentina. In this study, five ETEC isolates from diarrheic dairy calves (2-10 d old) from Buenos Aires and Cordoba, Argentina were characterized on the basis of virulence gene (VG) pattern, O:H serotyping, hemolytic phenotype, phylogenetic group affiliation, antimicrobial (AM) resistance profile, and presence of integron class 1 and 2. The five isolates were examined by polymerase chain reaction (PCR) for the presence of 18 bovine VGs and showed the following genotypes: F5+/F41+/sta + (D242), F5+/sta + (D158), F5+/sta + (D157), F5+ (D151-9), and F5+/iucD + (D151-5). These VGs confer pathogenic potential and most of them are associated with the ETEC pathotype. The five isolates showed a non-hemolytic phenotype, belonged to five different serotypes: O101:H-, O141:H-, O60:H-, ONT:H10, and ONT:H-, and were assigned to the phylogenetic group A by the quadruplex Clermont PCR method. The AM resistance of the three isolates D242, D157, and D151-5 was determined by agar disk diffusion method for 24 AMs and they exhibited a multi-resistance phenotype (resistance to four different AM classes: Cephalosporins, Penicillins, Macrolides, and Ansamycins). In addition, class 1 integrons were found in the isolate D151-5 containing the dfrA17-aadA5 gene cassette and in the bovine ETEC reference strain FV10191 containing the dfrA1-aadA1 gene cassette. The present study revealed for the first time the occurrence of multi-resistant ETEC associated with neonatal diarrhea in dairy calves in Argentina. This finding may be used for diagnostic and therapeutic purposes. SN - 2226-4485 UR - https://www.unboundmedicine.com/medline/citation/31086769/Phenotypic_and_genotypic_characterization_of_enterotoxigenic_Escherichia_coli_isolated_from_diarrheic_calves_in_Argentina L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31086769/ DB - PRIME DP - Unbound Medicine ER -