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Unraveling spatiotemporal variability of arbuscular mycorrhizal fungi in a temperate grassland plot.
Environ Microbiol. 2020 03; 22(3):873-888.EM

Abstract

Soils provide a heterogeneous environment varying in space and time; consequently, the biodiversity of soil microorganisms also differs spatially and temporally. For soil microbes tightly associated with plant roots, such as arbuscular mycorrhizal fungi (AMF), the diversity of plant partners and seasonal variability in trophic exchanges between the symbionts introduce additional heterogeneity. To clarify the impact of such heterogeneity, we investigated spatiotemporal variation in AMF diversity on a plot scale (10 × 10 m) in a grassland managed at low intensity in southwest Germany. AMF diversity was determined using 18S rDNA pyrosequencing analysis of 360 soil samples taken at six time points within a year. We observed high AMF alpha- and beta-diversity across the plot and at all investigated time points. Relationships were detected between spatiotemporal variation in AMF OTU richness and plant species richness, root biomass, minimal changes in soil texture and pH. The plot was characterized by high AMF turnover rates with a positive spatiotemporal relationship for AMF beta-diversity. However, environmental variables explained only ≈20% of the variation in AMF communities. This indicates that the observed spatiotemporal richness and community variability of AMF was largely independent of the abiotic environment, but related to plant properties and the cooccurring microbiome.

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Authors+Show Affiliations

Goldmann K
Department of Soil Ecology, UFZ - Helmholtz Centre for Environmental Research, Theodor-Lieser-Straβe 4, 06120, Halle (Saale), Germany.
Boeddinghaus RS
Department of Soil Biology, Institute of Soil Science and Land Evaluation, University of Hohenheim, Emil-Wolff-Straβe 27, 70599, Stuttgart, Germany.
Klemmer S
Department of Soil Ecology, UFZ - Helmholtz Centre for Environmental Research, Theodor-Lieser-Straβe 4, 06120, Halle (Saale), Germany.
Regan KM
Department of Soil Biology, Institute of Soil Science and Land Evaluation, University of Hohenheim, Emil-Wolff-Straβe 27, 70599, Stuttgart, Germany. Ecosystems Center, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA, 02543, USA.
Heintz-Buschart A
Department of Soil Ecology, UFZ - Helmholtz Centre for Environmental Research, Theodor-Lieser-Straβe 4, 06120, Halle (Saale), Germany. German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 5e, 04103, Leipzig, Germany.
Fischer M
Institute of Plant Sciences and Botanical Garden, University of Bern, Altenbergrain 21, 3013, Bern, Switzerland.
Prati D
Institute of Plant Sciences and Botanical Garden, University of Bern, Altenbergrain 21, 3013, Bern, Switzerland.
Piepho HP
Institute of Crop Science, Biostatistics Unit, University of Hohenheim, Fruwirthstraβe 23, 70599, Stuttgart, Germany.
Berner D
Department of Soil Biology, Institute of Soil Science and Land Evaluation, University of Hohenheim, Emil-Wolff-Straβe 27, 70599, Stuttgart, Germany.
Marhan S
Department of Soil Biology, Institute of Soil Science and Land Evaluation, University of Hohenheim, Emil-Wolff-Straβe 27, 70599, Stuttgart, Germany.
Kandeler E
Department of Soil Biology, Institute of Soil Science and Land Evaluation, University of Hohenheim, Emil-Wolff-Straβe 27, 70599, Stuttgart, Germany.
Buscot F
Department of Soil Ecology, UFZ - Helmholtz Centre for Environmental Research, Theodor-Lieser-Straβe 4, 06120, Halle (Saale), Germany. German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 5e, 04103, Leipzig, Germany.
Wubet T
Department of Soil Ecology, UFZ - Helmholtz Centre for Environmental Research, Theodor-Lieser-Straβe 4, 06120, Halle (Saale), Germany. German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 5e, 04103, Leipzig, Germany.

MeSH

BiodiversityBiomassGermanyGrasslandMycorrhizaePlant RootsPlantsRNA, Ribosomal, 18SSeasonsSoilSoil Microbiology

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31087598

Citation

Goldmann, Kezia, et al. "Unraveling Spatiotemporal Variability of Arbuscular Mycorrhizal Fungi in a Temperate Grassland Plot." Environmental Microbiology, vol. 22, no. 3, 2020, pp. 873-888.
Goldmann K, Boeddinghaus RS, Klemmer S, et al. Unraveling spatiotemporal variability of arbuscular mycorrhizal fungi in a temperate grassland plot. Environ Microbiol. 2020;22(3):873-888.
Goldmann, K., Boeddinghaus, R. S., Klemmer, S., Regan, K. M., Heintz-Buschart, A., Fischer, M., Prati, D., Piepho, H. P., Berner, D., Marhan, S., Kandeler, E., Buscot, F., & Wubet, T. (2020). Unraveling spatiotemporal variability of arbuscular mycorrhizal fungi in a temperate grassland plot. Environmental Microbiology, 22(3), 873-888. https://doi.org/10.1111/1462-2920.14653
Goldmann K, et al. Unraveling Spatiotemporal Variability of Arbuscular Mycorrhizal Fungi in a Temperate Grassland Plot. Environ Microbiol. 2020;22(3):873-888. PubMed PMID: 31087598.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Unraveling spatiotemporal variability of arbuscular mycorrhizal fungi in a temperate grassland plot. AU - Goldmann,Kezia, AU - Boeddinghaus,Runa S, AU - Klemmer,Sandra, AU - Regan,Kathleen M, AU - Heintz-Buschart,Anna, AU - Fischer,Markus, AU - Prati,Daniel, AU - Piepho,Hans-Peter, AU - Berner,Doreen, AU - Marhan,Sven, AU - Kandeler,Ellen, AU - Buscot,François, AU - Wubet,Tesfaye, Y1 - 2019/06/27/ PY - 2018/11/18/received PY - 2019/05/09/revised PY - 2019/05/10/accepted PY - 2019/5/16/pubmed PY - 2020/12/15/medline PY - 2019/5/16/entrez SP - 873 EP - 888 JF - Environmental microbiology JO - Environ Microbiol VL - 22 IS - 3 N2 - Soils provide a heterogeneous environment varying in space and time; consequently, the biodiversity of soil microorganisms also differs spatially and temporally. For soil microbes tightly associated with plant roots, such as arbuscular mycorrhizal fungi (AMF), the diversity of plant partners and seasonal variability in trophic exchanges between the symbionts introduce additional heterogeneity. To clarify the impact of such heterogeneity, we investigated spatiotemporal variation in AMF diversity on a plot scale (10 × 10 m) in a grassland managed at low intensity in southwest Germany. AMF diversity was determined using 18S rDNA pyrosequencing analysis of 360 soil samples taken at six time points within a year. We observed high AMF alpha- and beta-diversity across the plot and at all investigated time points. Relationships were detected between spatiotemporal variation in AMF OTU richness and plant species richness, root biomass, minimal changes in soil texture and pH. The plot was characterized by high AMF turnover rates with a positive spatiotemporal relationship for AMF beta-diversity. However, environmental variables explained only ≈20% of the variation in AMF communities. This indicates that the observed spatiotemporal richness and community variability of AMF was largely independent of the abiotic environment, but related to plant properties and the cooccurring microbiome. SN - 1462-2920 UR - https://www.unboundmedicine.com/medline/citation/31087598/Unraveling_spatiotemporal_variability_of_arbuscular_mycorrhizal_fungi_in_a_temperate_grassland_plot_ DB - PRIME DP - Unbound Medicine ER -