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Identification of plasma microRNA expression changes in multiple system atrophy and Parkinson's disease.
Mol Brain. 2019 05 14; 12(1):49.MB

Abstract

MicroRNAs (miRNAs) are endogenous small (18-25 nt), single-stranded, non-coding RNAs that play key roles in post-transcriptional gene expression regulation. The expression profiles of miRNAs in biofluids and tissues change in various diseases. Multiple system atrophy (MSA) and Parkinson's disease (PD) are both categorized as α-synucleinopathies and often present with similar clinical manifestations. This study aimed to identify miRNAs that are differently expressed in plasma samples of PD patients, MSA patients, and healthy controls. We used microarray analysis to screen for miRNAs that are up- and down-regulated in these patients and analyzed the relative-quantitative expression levels of the identified miRNAs by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Hsa-miR-671-5p, hsa-miR-19b-3p, and hsa-miR-24-3p showed significantly different expression levels among patients with MSA-C, MSA-P, or PD, and healthy controls. Hsa-miR-671-5p levels were lower in the MSA-P and PD than the MSA-C and control groups, hsa-miR-19b-3p levels were higher in the PD than the other groups, and hsa-miR-24-3p levels were higher in the PD than the MSA-C group. Hsa-miR-671-5p was the first miRNA shown to be expressed differently between MSA-C and MSA-P in plasma. Interestingly, the expression levels of hsa-miR-19b-3p and hsa-miR-24-3p were positively correlated, indicating that these miRNAs may be involved in the same processes in PD pathogenesis. Our findings suggest that hsa-miR-671-5p, hsa-miR-19b-3p, and hsa-miR-24-3p may reflect the pathophysiology or symptoms of PD and MSA.

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Authors+Show Affiliations

Department of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, North 15 West 7, Kita-Ku, Sapporo, Hokkaido, 060-8368, Japan. uwatoko@pop.med.hokudai.ac.jp.

Department of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, North 15 West 7, Kita-Ku, Sapporo, Hokkaido, 060-8368, Japan.

Department of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, North 15 West 7, Kita-Ku, Sapporo, Hokkaido, 060-8368, Japan.

Department of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, North 15 West 7, Kita-Ku, Sapporo, Hokkaido, 060-8368, Japan.

Department of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, North 15 West 7, Kita-Ku, Sapporo, Hokkaido, 060-8368, Japan.

Department of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, North 15 West 7, Kita-Ku, Sapporo, Hokkaido, 060-8368, Japan.

Department of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, North 15 West 7, Kita-Ku, Sapporo, Hokkaido, 060-8368, Japan.

Department of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, North 15 West 7, Kita-Ku, Sapporo, Hokkaido, 060-8368, Japan.

MeSH

Case-Control StudiesDown-RegulationFemaleGene Expression RegulationGene OntologyHumansMaleMicroRNAsMiddle AgedMultiple System AtrophyParkinson DiseaseUp-Regulation

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31088501

Citation

Uwatoko, Hisashi, et al. "Identification of Plasma microRNA Expression Changes in Multiple System Atrophy and Parkinson's Disease." Molecular Brain, vol. 12, no. 1, 2019, p. 49.

Uwatoko H, Hama Y, Iwata IT, et al. Identification of plasma microRNA expression changes in multiple system atrophy and Parkinson's disease. Mol Brain. 2019;12(1):49.

Uwatoko, H., Hama, Y., Iwata, I. T., Shirai, S., Matsushima, M., Yabe, I., Utsumi, J., & Sasaki, H. (2019). Identification of plasma microRNA expression changes in multiple system atrophy and Parkinson's disease. Molecular Brain, 12(1), 49. https://doi.org/10.1186/s13041-019-0471-2

Uwatoko H, et al. Identification of Plasma microRNA Expression Changes in Multiple System Atrophy and Parkinson's Disease. Mol Brain. 2019 05 14;12(1):49. PubMed PMID: 31088501.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Identification of plasma microRNA expression changes in multiple system atrophy and Parkinson's disease. AU - Uwatoko,Hisashi, AU - Hama,Yuka, AU - Iwata,Ikuko Takahashi, AU - Shirai,Shinichi, AU - Matsushima,Masaaki, AU - Yabe,Ichiro, AU - Utsumi,Jun, AU - Sasaki,Hidenao, Y1 - 2019/05/14/ PY - 2019/01/30/received PY - 2019/05/01/accepted PY - 2019/5/16/entrez PY - 2019/5/16/pubmed PY - 2020/5/27/medline KW - Hsa-miR-19b-3p KW - Hsa-miR-24-3p KW - Hsa-miR-671-5p KW - Microarray KW - Multiple system atrophy KW - Parkinson’s disease KW - Plasma KW - Quantitative polymerase chain reaction KW - microRNA SP - 49 EP - 49 JF - Molecular brain JO - Mol Brain VL - 12 IS - 1 N2 - MicroRNAs (miRNAs) are endogenous small (18-25 nt), single-stranded, non-coding RNAs that play key roles in post-transcriptional gene expression regulation. The expression profiles of miRNAs in biofluids and tissues change in various diseases. Multiple system atrophy (MSA) and Parkinson's disease (PD) are both categorized as α-synucleinopathies and often present with similar clinical manifestations. This study aimed to identify miRNAs that are differently expressed in plasma samples of PD patients, MSA patients, and healthy controls. We used microarray analysis to screen for miRNAs that are up- and down-regulated in these patients and analyzed the relative-quantitative expression levels of the identified miRNAs by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Hsa-miR-671-5p, hsa-miR-19b-3p, and hsa-miR-24-3p showed significantly different expression levels among patients with MSA-C, MSA-P, or PD, and healthy controls. Hsa-miR-671-5p levels were lower in the MSA-P and PD than the MSA-C and control groups, hsa-miR-19b-3p levels were higher in the PD than the other groups, and hsa-miR-24-3p levels were higher in the PD than the MSA-C group. Hsa-miR-671-5p was the first miRNA shown to be expressed differently between MSA-C and MSA-P in plasma. Interestingly, the expression levels of hsa-miR-19b-3p and hsa-miR-24-3p were positively correlated, indicating that these miRNAs may be involved in the same processes in PD pathogenesis. Our findings suggest that hsa-miR-671-5p, hsa-miR-19b-3p, and hsa-miR-24-3p may reflect the pathophysiology or symptoms of PD and MSA. SN - 1756-6606 UR - https://www.unboundmedicine.com/medline/citation/31088501/Identification_of_plasma_microRNA_expression_changes_in_multiple_system_atrophy_and_Parkinson's_disease_ DB - PRIME DP - Unbound Medicine ER -