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Role of gamma-giardin in ventral disc formation of Giardia lamblia.
Parasit Vectors 2019; 12(1):227PV

Abstract

BACKGROUND

Giardia lamblia, a protozoan pathogen causing diarrheal outbreaks, has characteristic cytoskeletal structures including eight flagella, a median body and a ventral disc. Gamma-giardin is a unique component protein of the cytoskeleton of this protozoan.

RESULTS

Through comparative proteomic analysis between different stages of the cell cycle, G. lamblia γ-giardin (Glγ-giardin) was identified as an upregulated protein in the G2-phase. Increased Glγ-giardin expression in G2 was confirmed by western blot and real-time polymerase chain reaction analyses. Knockdown of this protein using a morpholino affected the formation of ventral discs, especially the microribbons of the discs, but exerted little effect on the binding ability of G. lamblia. The number of cells with four nuclei was increased in Glγ-giardin-knockdown cells. Expression of Glγ-giardin was decreased during encystation, in contrast with the G2-phase.

CONCLUSIONS

Knockdown experiments demonstrated that Glγ-giardin is a component of the trilaminar structure of the ventral disc. Expression of Glγ-giardin is induced in the G2-phase prior to active cell division, whereas its expression decreases during encystation, a dormant stage of G. lamblia.

Authors+Show Affiliations

Department of Environmental Medical Biology and Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul, South Korea.Department of Environmental Medical Biology and Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul, South Korea. sjpark615@yuhs.ac.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31088539

Citation

Kim, Juri, and Soon-Jung Park. "Role of Gamma-giardin in Ventral Disc Formation of Giardia Lamblia." Parasites & Vectors, vol. 12, no. 1, 2019, p. 227.
Kim J, Park SJ. Role of gamma-giardin in ventral disc formation of Giardia lamblia. Parasit Vectors. 2019;12(1):227.
Kim, J., & Park, S. J. (2019). Role of gamma-giardin in ventral disc formation of Giardia lamblia. Parasites & Vectors, 12(1), p. 227. doi:10.1186/s13071-019-3478-8.
Kim J, Park SJ. Role of Gamma-giardin in Ventral Disc Formation of Giardia Lamblia. Parasit Vectors. 2019 May 14;12(1):227. PubMed PMID: 31088539.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of gamma-giardin in ventral disc formation of Giardia lamblia. AU - Kim,Juri, AU - Park,Soon-Jung, Y1 - 2019/05/14/ PY - 2019/01/06/received PY - 2019/05/03/accepted PY - 2019/5/16/entrez PY - 2019/5/16/pubmed PY - 2019/6/25/medline KW - Cell-cycle control KW - Gamma-giardin KW - Giardia lamblia KW - Ventral disc SP - 227 EP - 227 JF - Parasites & vectors JO - Parasit Vectors VL - 12 IS - 1 N2 - BACKGROUND: Giardia lamblia, a protozoan pathogen causing diarrheal outbreaks, has characteristic cytoskeletal structures including eight flagella, a median body and a ventral disc. Gamma-giardin is a unique component protein of the cytoskeleton of this protozoan. RESULTS: Through comparative proteomic analysis between different stages of the cell cycle, G. lamblia γ-giardin (Glγ-giardin) was identified as an upregulated protein in the G2-phase. Increased Glγ-giardin expression in G2 was confirmed by western blot and real-time polymerase chain reaction analyses. Knockdown of this protein using a morpholino affected the formation of ventral discs, especially the microribbons of the discs, but exerted little effect on the binding ability of G. lamblia. The number of cells with four nuclei was increased in Glγ-giardin-knockdown cells. Expression of Glγ-giardin was decreased during encystation, in contrast with the G2-phase. CONCLUSIONS: Knockdown experiments demonstrated that Glγ-giardin is a component of the trilaminar structure of the ventral disc. Expression of Glγ-giardin is induced in the G2-phase prior to active cell division, whereas its expression decreases during encystation, a dormant stage of G. lamblia. SN - 1756-3305 UR - https://www.unboundmedicine.com/medline/citation/31088539/Role_of_gamma-giardin_in_ventral_disc_formation_of_Giardia_lamblia L2 - https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-019-3478-8 DB - PRIME DP - Unbound Medicine ER -