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Novel electrospun poly(ε-caprolactone)/type I collagen nanofiber conduits for repair of peripheral nerve injury.
Neural Regen Res 2019; 14(9):1617-1625NR

Abstract

Recent studies have shown the potential of artificially synthesized conduits in the repair of peripheral nerve injury. Natural biopolymers have received much attention because of their biocompatibility. To investigate the effects of novel electrospun absorbable poly(ε-caprolactone)/type I collagen nanofiber conduits (biopolymer nanofiber conduits) on the repair of peripheral nerve injury, we bridged 10-mm-long sciatic nerve defects with electrospun absorbable biopolymer nanofiber conduits, poly(ε-caprolactone) or silicone conduits in Sprague-Dawley rats. Rat neurologica1 function was weekly evaluated using sciatic function index within 8 weeks after repair. Eight weeks after repair, sciatic nerve myelin sheaths and axon morphology were observed by osmium tetroxide staining, hematoxylin-eosin staining, and transmission electron microscopy. S-100 (Schwann cell marker) and CD4 (inflammatory marker) immunoreactivities in sciatic nerve were detected by immunohistochemistry. In rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits, no serious inflammatory reactions were observed in rat hind limbs, the morphology of myelin sheaths in the injured sciatic nerve was close to normal. CD4 immunoreactivity was obviously weaker in rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits than in those subjected to repair with poly(ε-caprolactone) or silicone. Rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits tended to have greater sciatic nerve function recovery than those receiving poly(ε-caprolactone) or silicone repair. These results suggest that electrospun absorbable poly(ε-caprolactone)/type I collagen nanofiber conduits have the potential of repairing sciatic nerve defects and exhibit good biocompatibility. All experimental procedures were approved by Institutional Animal Care and Use Committee of Taichung Veteran General Hospital, Taiwan, China (La-1031218) on October 2, 2014.

Authors+Show Affiliations

Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital; Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung, Taiwan, China.Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital; Department of Physical Therapy, Hungkuang University; Basic Medical Education Center, Central Taiwan University of Science and Technology, Taichung, Taiwan, China.Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung, Taiwan, China.Department of Medical Imaging and Radiological Sciences, Central Taiwan University of Science and Technology, Taichung, Taiwan, China.Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung, Taiwan, China.Institute of Biomedical Sciences, National Chung Hsing University; Department of Medical Research, Taichung Veterans General Hospital; Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, Taiwan, China.Department of Management Information System, National Chung Hsing University, Taichung, Taiwan, China.Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital; Department of Physical Therapy, Hungkuang University, Taichung, Taiwan, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31089062

Citation

Yen, Chun-Ming, et al. "Novel Electrospun Poly(ε-caprolactone)/type I Collagen Nanofiber Conduits for Repair of Peripheral Nerve Injury." Neural Regeneration Research, vol. 14, no. 9, 2019, pp. 1617-1625.
Yen CM, Shen CC, Yang YC, et al. Novel electrospun poly(ε-caprolactone)/type I collagen nanofiber conduits for repair of peripheral nerve injury. Neural Regen Res. 2019;14(9):1617-1625.
Yen, C. M., Shen, C. C., Yang, Y. C., Liu, B. S., Lee, H. T., Sheu, M. L., ... Cheng, W. Y. (2019). Novel electrospun poly(ε-caprolactone)/type I collagen nanofiber conduits for repair of peripheral nerve injury. Neural Regeneration Research, 14(9), pp. 1617-1625. doi:10.4103/1673-5374.255997.
Yen CM, et al. Novel Electrospun Poly(ε-caprolactone)/type I Collagen Nanofiber Conduits for Repair of Peripheral Nerve Injury. Neural Regen Res. 2019;14(9):1617-1625. PubMed PMID: 31089062.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel electrospun poly(ε-caprolactone)/type I collagen nanofiber conduits for repair of peripheral nerve injury. AU - Yen,Chun-Ming, AU - Shen,Chiung-Chyi, AU - Yang,Yi-Chin, AU - Liu,Bai-Shuan, AU - Lee,Hsu-Tung, AU - Sheu,Meei-Ling, AU - Tsai,Meng-Hsiun, AU - Cheng,Wen-Yu, PY - 2019/5/16/entrez PY - 2019/5/16/pubmed PY - 2019/5/16/medline KW - electrospinning KW - immunohistostaining KW - nerve conduit KW - peripheral nerve injury KW - poly(ε-caprolactone) KW - sciatic nerve KW - type I collagen KW - walking track analysis SP - 1617 EP - 1625 JF - Neural regeneration research JO - Neural Regen Res VL - 14 IS - 9 N2 - Recent studies have shown the potential of artificially synthesized conduits in the repair of peripheral nerve injury. Natural biopolymers have received much attention because of their biocompatibility. To investigate the effects of novel electrospun absorbable poly(ε-caprolactone)/type I collagen nanofiber conduits (biopolymer nanofiber conduits) on the repair of peripheral nerve injury, we bridged 10-mm-long sciatic nerve defects with electrospun absorbable biopolymer nanofiber conduits, poly(ε-caprolactone) or silicone conduits in Sprague-Dawley rats. Rat neurologica1 function was weekly evaluated using sciatic function index within 8 weeks after repair. Eight weeks after repair, sciatic nerve myelin sheaths and axon morphology were observed by osmium tetroxide staining, hematoxylin-eosin staining, and transmission electron microscopy. S-100 (Schwann cell marker) and CD4 (inflammatory marker) immunoreactivities in sciatic nerve were detected by immunohistochemistry. In rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits, no serious inflammatory reactions were observed in rat hind limbs, the morphology of myelin sheaths in the injured sciatic nerve was close to normal. CD4 immunoreactivity was obviously weaker in rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits than in those subjected to repair with poly(ε-caprolactone) or silicone. Rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits tended to have greater sciatic nerve function recovery than those receiving poly(ε-caprolactone) or silicone repair. These results suggest that electrospun absorbable poly(ε-caprolactone)/type I collagen nanofiber conduits have the potential of repairing sciatic nerve defects and exhibit good biocompatibility. All experimental procedures were approved by Institutional Animal Care and Use Committee of Taichung Veteran General Hospital, Taiwan, China (La-1031218) on October 2, 2014. SN - 1673-5374 UR - https://www.unboundmedicine.com/medline/citation/31089062/Novel_electrospun_poly(ε-caprolactone)/type_I_collagen_nanofiber_conduits_for_repair_of_peripheral_nerve_injury L2 - http://www.nrronline.org/article.asp?issn=1673-5374;year=2019;volume=14;issue=9;spage=1617;epage=1625;aulast=Yen DB - PRIME DP - Unbound Medicine ER -