Tags

Type your tag names separated by a space and hit enter

[Expression of gamma-aminobutyric acid type A receptor beta3 subunit in murine cleft palate induced by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin].

Abstract

Objective:

To investigate the expression of gamma-aminobutyric acid type A receptor beta3 subunit (GABRB3) on cleft palate in C57BL/6J mice induced by 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD).

Methods:

Sixty C57BL/6J pregnant mice on gestation day (GD) 10.5 were divided into two groups: one group was administered through gastric tubes one dose of 28 μg/kg TCDD (experimental group) and the other group was administered through gastric tubes one dose of 5.6 ml/kg corn oil (control group). Embryos were removed by cesarean section from pregnant mice during the palatal formation stage (GD 13.5-17.5) and the palatal tissue studied in morphological and histological observation. The relative mRNA and protein expression of GABRB3 was measured by real-time quantitative PCR and Western blotting. Localization of GABRB3 protein was measured by immunohistochemistry or immunofluorescence.

Results:

The incidence of cleft palate at GD17.5 was 100% in experimental group and there was no cleft palate occurred in the control group (0); elevation of palatine processes in experimental group was completed on GD15.5 which was clearly delayed by a day compared with that in control group. On GD14.5-GD17.5, the mRNA expression (0.561±0.073, 0.728±0.104, 0.782±0.137, 0.686±0.145) and protein expression (0.288±0.013, 0.404±0.017, 0.399±0.012, 0.307±0.010) in the experimental group were significantly lower than the control group mRNA expression (0.818±0.088, 0.865±0.086, 1.021±0.054, 1.163±0.179) and protein expression (0.481±0.017, 0.456±0.009, 0.474±0.016, 0.529±0.015)(P<0.05). Immunohistochemistry and immunofluorescence showed that GABRB3 was mainly expressed in the mesenchymal cells and medial edge epithelium.

Conclusions:

TCDD delayed palatal shelf elevation and eventually led to cleft palate may be associated with a decrease in GABRB3. GABRB3 may play an important role in the elevation and fusion phases of the palate development.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Burns and Plastic Surgery, Children's Hospital of Chongqing Medical University & Ministry of Education Key Laboratory of Child Development and Disorders & China International Science and Technology Cooperation Base of Child Development and Critical Disorders & Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China.

    , , , ,

    Source

    Pub Type(s)

    English Abstract
    Journal Article

    Language

    chi

    PubMed ID

    31091566

    Citation

    Lei, J Q., et al. "[Expression of Gamma-aminobutyric Acid Type a Receptor Beta3 Subunit in Murine Cleft Palate Induced By 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin]." Zhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal of Stomatology, vol. 54, no. 5, 2019, pp. 328-334.
    Lei JQ, Qiu L, Ding XH, et al. [Expression of gamma-aminobutyric acid type A receptor beta3 subunit in murine cleft palate induced by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2019;54(5):328-334.
    Lei, J. Q., Qiu, L., Ding, X. H., Fu, Y. X., Yuan, X. G., & Liu, Y. (2019). [Expression of gamma-aminobutyric acid type A receptor beta3 subunit in murine cleft palate induced by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin]. Zhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal of Stomatology, 54(5), pp. 328-334. doi:10.3760/cma.j.issn.1002-0098.2019.05.007.
    Lei JQ, et al. [Expression of Gamma-aminobutyric Acid Type a Receptor Beta3 Subunit in Murine Cleft Palate Induced By 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2019 May 9;54(5):328-334. PubMed PMID: 31091566.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - [Expression of gamma-aminobutyric acid type A receptor beta3 subunit in murine cleft palate induced by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin]. AU - Lei,J Q, AU - Qiu,L, AU - Ding,X H, AU - Fu,Y X, AU - Yuan,X G, AU - Liu,Y, PY - 2019/5/16/entrez KW - Cleft palate KW - Gamma-aminobutyric acid type A receptor beta3 subunit KW - Tetrachlorodibenzodioxin SP - 328 EP - 334 JF - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology JO - Zhonghua Kou Qiang Yi Xue Za Zhi VL - 54 IS - 5 N2 - Objective: To investigate the expression of gamma-aminobutyric acid type A receptor beta3 subunit (GABRB3) on cleft palate in C57BL/6J mice induced by 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD). Methods: Sixty C57BL/6J pregnant mice on gestation day (GD) 10.5 were divided into two groups: one group was administered through gastric tubes one dose of 28 μg/kg TCDD (experimental group) and the other group was administered through gastric tubes one dose of 5.6 ml/kg corn oil (control group). Embryos were removed by cesarean section from pregnant mice during the palatal formation stage (GD 13.5-17.5) and the palatal tissue studied in morphological and histological observation. The relative mRNA and protein expression of GABRB3 was measured by real-time quantitative PCR and Western blotting. Localization of GABRB3 protein was measured by immunohistochemistry or immunofluorescence. Results: The incidence of cleft palate at GD17.5 was 100% in experimental group and there was no cleft palate occurred in the control group (0); elevation of palatine processes in experimental group was completed on GD15.5 which was clearly delayed by a day compared with that in control group. On GD14.5-GD17.5, the mRNA expression (0.561±0.073, 0.728±0.104, 0.782±0.137, 0.686±0.145) and protein expression (0.288±0.013, 0.404±0.017, 0.399±0.012, 0.307±0.010) in the experimental group were significantly lower than the control group mRNA expression (0.818±0.088, 0.865±0.086, 1.021±0.054, 1.163±0.179) and protein expression (0.481±0.017, 0.456±0.009, 0.474±0.016, 0.529±0.015)(P<0.05). Immunohistochemistry and immunofluorescence showed that GABRB3 was mainly expressed in the mesenchymal cells and medial edge epithelium. Conclusions: TCDD delayed palatal shelf elevation and eventually led to cleft palate may be associated with a decrease in GABRB3. GABRB3 may play an important role in the elevation and fusion phases of the palate development. SN - 1002-0098 UR - https://www.unboundmedicine.com/medline/citation/31091566/[Expression_of_gamma-aminobutyric_acid_type_A_receptor_beta3_subunit_in_murine_cleft_palate_induced_by_2,_3,_7,_8-tetrachlorodibenzo-p-dioxin] L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=1002-0098&amp;year=2019&amp;vol=54&amp;issue=5&amp;fpage=328 DB - PRIME DP - Unbound Medicine ER -