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[Expression of gamma-aminobutyric acid type A receptor beta3 subunit in murine cleft palate induced by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin].
Zhonghua Kou Qiang Yi Xue Za Zhi 2019; 54(5):328-334ZK

Abstract

Objective:

To investigate the expression of gamma-aminobutyric acid type A receptor beta3 subunit (GABRB3) on cleft palate in C57BL/6J mice induced by 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD).

Methods:

Sixty C57BL/6J pregnant mice on gestation day (GD) 10.5 were divided into two groups: one group was administered through gastric tubes one dose of 28 μg/kg TCDD (experimental group) and the other group was administered through gastric tubes one dose of 5.6 ml/kg corn oil (control group). Embryos were removed by cesarean section from pregnant mice during the palatal formation stage (GD 13.5-17.5) and the palatal tissue studied in morphological and histological observation. The relative mRNA and protein expression of GABRB3 was measured by real-time quantitative PCR and Western blotting. Localization of GABRB3 protein was measured by immunohistochemistry or immunofluorescence.

Results:

The incidence of cleft palate at GD17.5 was 100% in experimental group and there was no cleft palate occurred in the control group (0); elevation of palatine processes in experimental group was completed on GD15.5 which was clearly delayed by a day compared with that in control group. On GD14.5-GD17.5, the mRNA expression (0.561±0.073, 0.728±0.104, 0.782±0.137, 0.686±0.145) and protein expression (0.288±0.013, 0.404±0.017, 0.399±0.012, 0.307±0.010) in the experimental group were significantly lower than the control group mRNA expression (0.818±0.088, 0.865±0.086, 1.021±0.054, 1.163±0.179) and protein expression (0.481±0.017, 0.456±0.009, 0.474±0.016, 0.529±0.015)(P<0.05). Immunohistochemistry and immunofluorescence showed that GABRB3 was mainly expressed in the mesenchymal cells and medial edge epithelium.

Conclusions:

TCDD delayed palatal shelf elevation and eventually led to cleft palate may be associated with a decrease in GABRB3. GABRB3 may play an important role in the elevation and fusion phases of the palate development.

Authors+Show Affiliations

Department of Burns and Plastic Surgery, Children's Hospital of Chongqing Medical University & Ministry of Education Key Laboratory of Child Development and Disorders & China International Science and Technology Cooperation Base of Child Development and Critical Disorders & Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31091566

Citation

Lei, J Q., et al. "[Expression of Gamma-aminobutyric Acid Type a Receptor Beta3 Subunit in Murine Cleft Palate Induced By 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin]." Zhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal of Stomatology, vol. 54, no. 5, 2019, pp. 328-334.
Lei JQ, Qiu L, Ding XH, et al. [Expression of gamma-aminobutyric acid type A receptor beta3 subunit in murine cleft palate induced by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2019;54(5):328-334.
Lei, J. Q., Qiu, L., Ding, X. H., Fu, Y. X., Yuan, X. G., & Liu, Y. (2019). [Expression of gamma-aminobutyric acid type A receptor beta3 subunit in murine cleft palate induced by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin]. Zhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal of Stomatology, 54(5), pp. 328-334. doi:10.3760/cma.j.issn.1002-0098.2019.05.007.
Lei JQ, et al. [Expression of Gamma-aminobutyric Acid Type a Receptor Beta3 Subunit in Murine Cleft Palate Induced By 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2019 May 9;54(5):328-334. PubMed PMID: 31091566.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression of gamma-aminobutyric acid type A receptor beta3 subunit in murine cleft palate induced by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin]. AU - Lei,J Q, AU - Qiu,L, AU - Ding,X H, AU - Fu,Y X, AU - Yuan,X G, AU - Liu,Y, PY - 2019/5/16/entrez PY - 2019/5/17/pubmed PY - 2019/5/17/medline KW - Cleft palate KW - Gamma-aminobutyric acid type A receptor beta3 subunit KW - Tetrachlorodibenzodioxin SP - 328 EP - 334 JF - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology JO - Zhonghua Kou Qiang Yi Xue Za Zhi VL - 54 IS - 5 N2 - Objective: To investigate the expression of gamma-aminobutyric acid type A receptor beta3 subunit (GABRB3) on cleft palate in C57BL/6J mice induced by 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD). Methods: Sixty C57BL/6J pregnant mice on gestation day (GD) 10.5 were divided into two groups: one group was administered through gastric tubes one dose of 28 μg/kg TCDD (experimental group) and the other group was administered through gastric tubes one dose of 5.6 ml/kg corn oil (control group). Embryos were removed by cesarean section from pregnant mice during the palatal formation stage (GD 13.5-17.5) and the palatal tissue studied in morphological and histological observation. The relative mRNA and protein expression of GABRB3 was measured by real-time quantitative PCR and Western blotting. Localization of GABRB3 protein was measured by immunohistochemistry or immunofluorescence. Results: The incidence of cleft palate at GD17.5 was 100% in experimental group and there was no cleft palate occurred in the control group (0); elevation of palatine processes in experimental group was completed on GD15.5 which was clearly delayed by a day compared with that in control group. On GD14.5-GD17.5, the mRNA expression (0.561±0.073, 0.728±0.104, 0.782±0.137, 0.686±0.145) and protein expression (0.288±0.013, 0.404±0.017, 0.399±0.012, 0.307±0.010) in the experimental group were significantly lower than the control group mRNA expression (0.818±0.088, 0.865±0.086, 1.021±0.054, 1.163±0.179) and protein expression (0.481±0.017, 0.456±0.009, 0.474±0.016, 0.529±0.015)(P<0.05). Immunohistochemistry and immunofluorescence showed that GABRB3 was mainly expressed in the mesenchymal cells and medial edge epithelium. Conclusions: TCDD delayed palatal shelf elevation and eventually led to cleft palate may be associated with a decrease in GABRB3. GABRB3 may play an important role in the elevation and fusion phases of the palate development. SN - 1002-0098 UR - https://www.unboundmedicine.com/medline/citation/31091566/[Expression_of_gamma-aminobutyric_acid_type_A_receptor_beta3_subunit_in_murine_cleft_palate_induced_by_2,_3,_7,_8-tetrachlorodibenzo-p-dioxin] L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=1002-0098&amp;year=2019&amp;vol=54&amp;issue=5&amp;fpage=328 DB - PRIME DP - Unbound Medicine ER -