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Otopetrin-2 Immunolocalization in the Human Macula Utricle.

Abstract

BACKGROUND

In the present study, we investigated the localization of otopetrin-2-a member of the otopetrin family that encodes proton-selective ion channels-in the human macula utricle using immunohistochemistry.

METHODS

Macula utricle were acquired at surgery from patients who required transmastoid labyrinthectomy for intractable vertigo due to Meniere's disease (MD; n = 3) and/or vestibular drops attacks (VDA; n = 2) and from temporal bones (n = 2) acquired at autopsy from individuals with no balance disorders. Immunofluorescence staining with otopetrin-2 (rabbit affinity purified polyclonal antibody) and GFAP (mouse monoclonal antibody) to identify vestibular supporting cells was made in formalin fixed cryostat sections or whole microdissected utricle (for flat mount preparations). Secondary antibodies against rabbit and mouse were used for the identification of both proteins. Digital fluorescent images were obtained using a high-resolution laser confocal microscope.

RESULTS

Using cryostat sections and flat mount preparations otopetrin-2 immunofluorescence was seen as punctated signal throughout the supporting cells cytoplasm. GFAP immunofluorescence was present in the supporting cell cytoplasm. The distribution of otopetrin-2 was similar in the macula utricle obtained from MD, VDA, or autopsy normative patients.

CONCLUSIONS

Otopetrin-2 was localized in supporting cells in a similar fashion that otopetrin-1 previously reported in the mouse macula utricle. The differential expression of otopetrin-2 in the supporting cells of the human macula utricle suggest an important role in the vestibular sensory periphery homeostasis and otolith maintenance.

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  • Authors+Show Affiliations

    ,

    1 Department of Head & Neck Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

    ,

    2 Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

    ,

    1 Department of Head & Neck Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

    1 Department of Head & Neck Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

    Source

    The Annals of otology, rhinology, and laryngology 128:6_suppl 2019 Jun pg 96S-102S

    MeSH

    Acoustic Maculae
    Aged
    Case-Control Studies
    Female
    Humans
    Male
    Membrane Proteins
    Meniere Disease
    Middle Aged
    Phosphoproteins
    Vestibular Diseases

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31092032

    Citation

    Lopez PhD, Ivan A., et al. "Otopetrin-2 Immunolocalization in the Human Macula Utricle." The Annals of Otology, Rhinology, and Laryngology, vol. 128, no. 6_suppl, 2019, 96S-102S.
    Lopez PhD IA, Ishiyama Md G, Acuna MSc D, et al. Otopetrin-2 Immunolocalization in the Human Macula Utricle. Ann Otol Rhinol Laryngol. 2019;128(6_suppl):96S-102S.
    Lopez PhD, I. A., Ishiyama Md, G., Acuna MSc, D., & Ishiyama Md, A. (2019). Otopetrin-2 Immunolocalization in the Human Macula Utricle. The Annals of Otology, Rhinology, and Laryngology, 128(6_suppl), 96S-102S. doi:10.1177/0003489419834952.
    Lopez PhD IA, et al. Otopetrin-2 Immunolocalization in the Human Macula Utricle. Ann Otol Rhinol Laryngol. 2019;128(6_suppl):96S-102S. PubMed PMID: 31092032.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Otopetrin-2 Immunolocalization in the Human Macula Utricle. AU - Lopez PhD,Ivan A, AU - Ishiyama Md,Gail, AU - Acuna MSc,Dora, AU - Ishiyama Md,Akira, PY - 2019/5/17/entrez PY - 2019/5/17/pubmed PY - 2019/5/29/medline KW - balance disorders KW - otolith KW - otopetrin-2 KW - supporting cells KW - utricle KW - vestibular hair cells SP - 96S EP - 102S JF - The Annals of otology, rhinology, and laryngology JO - Ann. Otol. Rhinol. Laryngol. VL - 128 IS - 6_suppl N2 - BACKGROUND: In the present study, we investigated the localization of otopetrin-2-a member of the otopetrin family that encodes proton-selective ion channels-in the human macula utricle using immunohistochemistry. METHODS: Macula utricle were acquired at surgery from patients who required transmastoid labyrinthectomy for intractable vertigo due to Meniere's disease (MD; n = 3) and/or vestibular drops attacks (VDA; n = 2) and from temporal bones (n = 2) acquired at autopsy from individuals with no balance disorders. Immunofluorescence staining with otopetrin-2 (rabbit affinity purified polyclonal antibody) and GFAP (mouse monoclonal antibody) to identify vestibular supporting cells was made in formalin fixed cryostat sections or whole microdissected utricle (for flat mount preparations). Secondary antibodies against rabbit and mouse were used for the identification of both proteins. Digital fluorescent images were obtained using a high-resolution laser confocal microscope. RESULTS: Using cryostat sections and flat mount preparations otopetrin-2 immunofluorescence was seen as punctated signal throughout the supporting cells cytoplasm. GFAP immunofluorescence was present in the supporting cell cytoplasm. The distribution of otopetrin-2 was similar in the macula utricle obtained from MD, VDA, or autopsy normative patients. CONCLUSIONS: Otopetrin-2 was localized in supporting cells in a similar fashion that otopetrin-1 previously reported in the mouse macula utricle. The differential expression of otopetrin-2 in the supporting cells of the human macula utricle suggest an important role in the vestibular sensory periphery homeostasis and otolith maintenance. SN - 1943-572X UR - https://www.unboundmedicine.com/medline/citation/31092032/Otopetrin-2_Immunolocalization_in_the_Human_Macula_Utricle L2 - http://journals.sagepub.com/doi/full/10.1177/0003489419834952?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -