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Otopetrin-2 Immunolocalization in the Human Macula Utricle.
Ann Otol Rhinol Laryngol 2019; 128(6_suppl):96S-102SAO

Abstract

BACKGROUND

In the present study, we investigated the localization of otopetrin-2-a member of the otopetrin family that encodes proton-selective ion channels-in the human macula utricle using immunohistochemistry.

METHODS

Macula utricle were acquired at surgery from patients who required transmastoid labyrinthectomy for intractable vertigo due to Meniere's disease (MD; n = 3) and/or vestibular drops attacks (VDA; n = 2) and from temporal bones (n = 2) acquired at autopsy from individuals with no balance disorders. Immunofluorescence staining with otopetrin-2 (rabbit affinity purified polyclonal antibody) and GFAP (mouse monoclonal antibody) to identify vestibular supporting cells was made in formalin fixed cryostat sections or whole microdissected utricle (for flat mount preparations). Secondary antibodies against rabbit and mouse were used for the identification of both proteins. Digital fluorescent images were obtained using a high-resolution laser confocal microscope.

RESULTS

Using cryostat sections and flat mount preparations otopetrin-2 immunofluorescence was seen as punctated signal throughout the supporting cells cytoplasm. GFAP immunofluorescence was present in the supporting cell cytoplasm. The distribution of otopetrin-2 was similar in the macula utricle obtained from MD, VDA, or autopsy normative patients.

CONCLUSIONS

Otopetrin-2 was localized in supporting cells in a similar fashion that otopetrin-1 previously reported in the mouse macula utricle. The differential expression of otopetrin-2 in the supporting cells of the human macula utricle suggest an important role in the vestibular sensory periphery homeostasis and otolith maintenance.

Authors+Show Affiliations

1 Department of Head & Neck Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.2 Department of Neurology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.1 Department of Head & Neck Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.1 Department of Head & Neck Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31092032

Citation

Lopez, Ivan A., et al. "Otopetrin-2 Immunolocalization in the Human Macula Utricle." The Annals of Otology, Rhinology, and Laryngology, vol. 128, no. 6_suppl, 2019, 96S-102S.
Lopez IA, Ishiyama G, Acuna D, et al. Otopetrin-2 Immunolocalization in the Human Macula Utricle. Ann Otol Rhinol Laryngol. 2019;128(6_suppl):96S-102S.
Lopez, I. A., Ishiyama, G., Acuna, D., & Ishiyama, A. (2019). Otopetrin-2 Immunolocalization in the Human Macula Utricle. The Annals of Otology, Rhinology, and Laryngology, 128(6_suppl), 96S-102S. doi:10.1177/0003489419834952.
Lopez IA, et al. Otopetrin-2 Immunolocalization in the Human Macula Utricle. Ann Otol Rhinol Laryngol. 2019;128(6_suppl):96S-102S. PubMed PMID: 31092032.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Otopetrin-2 Immunolocalization in the Human Macula Utricle. AU - Lopez,Ivan A, AU - Ishiyama,Gail, AU - Acuna,Dora, AU - Ishiyama,Akira, PY - 2020/06/01/pmc-release PY - 2019/5/17/entrez PY - 2019/5/17/pubmed PY - 2019/5/29/medline KW - balance disorders KW - otolith KW - otopetrin-2 KW - supporting cells KW - utricle KW - vestibular hair cells SP - 96S EP - 102S JF - The Annals of otology, rhinology, and laryngology JO - Ann. Otol. Rhinol. Laryngol. VL - 128 IS - 6_suppl N2 - BACKGROUND: In the present study, we investigated the localization of otopetrin-2-a member of the otopetrin family that encodes proton-selective ion channels-in the human macula utricle using immunohistochemistry. METHODS: Macula utricle were acquired at surgery from patients who required transmastoid labyrinthectomy for intractable vertigo due to Meniere's disease (MD; n = 3) and/or vestibular drops attacks (VDA; n = 2) and from temporal bones (n = 2) acquired at autopsy from individuals with no balance disorders. Immunofluorescence staining with otopetrin-2 (rabbit affinity purified polyclonal antibody) and GFAP (mouse monoclonal antibody) to identify vestibular supporting cells was made in formalin fixed cryostat sections or whole microdissected utricle (for flat mount preparations). Secondary antibodies against rabbit and mouse were used for the identification of both proteins. Digital fluorescent images were obtained using a high-resolution laser confocal microscope. RESULTS: Using cryostat sections and flat mount preparations otopetrin-2 immunofluorescence was seen as punctated signal throughout the supporting cells cytoplasm. GFAP immunofluorescence was present in the supporting cell cytoplasm. The distribution of otopetrin-2 was similar in the macula utricle obtained from MD, VDA, or autopsy normative patients. CONCLUSIONS: Otopetrin-2 was localized in supporting cells in a similar fashion that otopetrin-1 previously reported in the mouse macula utricle. The differential expression of otopetrin-2 in the supporting cells of the human macula utricle suggest an important role in the vestibular sensory periphery homeostasis and otolith maintenance. SN - 1943-572X UR - https://www.unboundmedicine.com/medline/citation/31092032/Otopetrin-2_Immunolocalization_in_the_Human_Macula_Utricle L2 - http://journals.sagepub.com/doi/full/10.1177/0003489419834952?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -