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Novel haplotypes and networks of AVR-Pik alleles in Magnaporthe oryzae.
BMC Plant Biol 2019; 19(1):204BP

Abstract

BACKGROUND

Rice blast disease is one of the most destructive fungal disease of rice worldwide. The avirulence (AVR) genes of Magnaporthe oryzae are recognized by the cognate resistance (R) genes of rice and trigger race-specific resistance. The variation in AVR is one of the major drivers of new races. Detecting the variation in the AVR gene in isolates from a population of Magnaporthe oryzae collected from rice production fields will aid in evaluating the effectiveness of R genes in rice production areas. The Pik gene contains 5 R alleles (Pik, Pikh, Pikp, Pikm and Piks) corresponding to the AVR alleles (AVR-Pik/kh/kp/km/ks) of M. oryzae. The Pik gene specifically recognizes and prevents infections by isolates of M. oryzae that contain AVR-Pik. The molecular variation in AVR-Pik alleles of M. oryzae and Pik alleles of rice remains unclear.

RESULTS

We studied the possible evolutionary pathways of AVR-Pik alleles by analyzing their DNA sequence variation and assaying their avirulence to the cognate Pik alleles of resistance genes under field conditions in China. The results of PCR products from genomic DNA showed that 278 of the 366 isolates of M. oryzae collected from Yunnan Province, China, carried AVR-Pik alleles. Among the isolates from six regions of Yunnan, 66.7-90.3% carried AVR-Pik alleles. Moreover, 10 AVR-Pik haplotypes encoding five novel AVR-Pik variants were identified among 201 isolates. The AVR-Pik alleles evolved to virulent from avirulent forms via stepwise base substitution. These findings demonstrate that AVR-Pik alleles are under positive selection and that mutations are responsible for defeating race-specific resistant Pik alleles in nature.

CONCLUSIONS

We demonstrated the polymorphism and distribution of AVR-Pik alleles in Yunnan Province, China. By pathogenicity assays used to detect the function of the different haplotypes of AVR-Pik, for the first time, we showed the avoidance and stepwise evolution of AVR-Pik alleles in rice production areas of Yunnan. The functional AVR-Pik possesses diversified sequence structures and is under positive selection in nature.

Authors+Show Affiliations

Agricultural Environment and Resources Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, China. kmlijinbin@yahoo.com.Agricultural Environment and Resources Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, China.The Ministry of Education Key Laboratory for Agricultural Biodiversity and Pest Management, Yunnan Agricultural University, Kunming, China.Agricultural Environment and Resources Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, China.Agricultural Environment and Resources Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, China.Agricultural Environment and Resources Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31096914

Citation

Li, Jinbin, et al. "Novel Haplotypes and Networks of AVR-Pik Alleles in Magnaporthe Oryzae." BMC Plant Biology, vol. 19, no. 1, 2019, p. 204.
Li J, Wang Q, Li C, et al. Novel haplotypes and networks of AVR-Pik alleles in Magnaporthe oryzae. BMC Plant Biol. 2019;19(1):204.
Li, J., Wang, Q., Li, C., Bi, Y., Fu, X., & Wang, R. (2019). Novel haplotypes and networks of AVR-Pik alleles in Magnaporthe oryzae. BMC Plant Biology, 19(1), p. 204. doi:10.1186/s12870-019-1817-8.
Li J, et al. Novel Haplotypes and Networks of AVR-Pik Alleles in Magnaporthe Oryzae. BMC Plant Biol. 2019 May 16;19(1):204. PubMed PMID: 31096914.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel haplotypes and networks of AVR-Pik alleles in Magnaporthe oryzae. AU - Li,Jinbin, AU - Wang,Qun, AU - Li,Chengyun, AU - Bi,Yunqing, AU - Fu,Xue, AU - Wang,Raoquan, Y1 - 2019/05/16/ PY - 2019/03/20/received PY - 2019/05/02/accepted PY - 2019/5/18/entrez PY - 2019/5/18/pubmed PY - 2019/5/18/medline KW - AVR-Pik KW - Effector KW - Evolution KW - Magnaporthe oryzae SP - 204 EP - 204 JF - BMC plant biology JO - BMC Plant Biol. VL - 19 IS - 1 N2 - BACKGROUND: Rice blast disease is one of the most destructive fungal disease of rice worldwide. The avirulence (AVR) genes of Magnaporthe oryzae are recognized by the cognate resistance (R) genes of rice and trigger race-specific resistance. The variation in AVR is one of the major drivers of new races. Detecting the variation in the AVR gene in isolates from a population of Magnaporthe oryzae collected from rice production fields will aid in evaluating the effectiveness of R genes in rice production areas. The Pik gene contains 5 R alleles (Pik, Pikh, Pikp, Pikm and Piks) corresponding to the AVR alleles (AVR-Pik/kh/kp/km/ks) of M. oryzae. The Pik gene specifically recognizes and prevents infections by isolates of M. oryzae that contain AVR-Pik. The molecular variation in AVR-Pik alleles of M. oryzae and Pik alleles of rice remains unclear. RESULTS: We studied the possible evolutionary pathways of AVR-Pik alleles by analyzing their DNA sequence variation and assaying their avirulence to the cognate Pik alleles of resistance genes under field conditions in China. The results of PCR products from genomic DNA showed that 278 of the 366 isolates of M. oryzae collected from Yunnan Province, China, carried AVR-Pik alleles. Among the isolates from six regions of Yunnan, 66.7-90.3% carried AVR-Pik alleles. Moreover, 10 AVR-Pik haplotypes encoding five novel AVR-Pik variants were identified among 201 isolates. The AVR-Pik alleles evolved to virulent from avirulent forms via stepwise base substitution. These findings demonstrate that AVR-Pik alleles are under positive selection and that mutations are responsible for defeating race-specific resistant Pik alleles in nature. CONCLUSIONS: We demonstrated the polymorphism and distribution of AVR-Pik alleles in Yunnan Province, China. By pathogenicity assays used to detect the function of the different haplotypes of AVR-Pik, for the first time, we showed the avoidance and stepwise evolution of AVR-Pik alleles in rice production areas of Yunnan. The functional AVR-Pik possesses diversified sequence structures and is under positive selection in nature. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/31096914/Novel_haplotypes_and_networks_of_AVR_Pik_alleles_in_Magnaporthe_oryzae_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-019-1817-8 DB - PRIME DP - Unbound Medicine ER -