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Purification of aconitase from Bacillus subtilis and correlation of its N-terminal amino acid sequence with the sequence of the citB gene.
J Bacteriol. 1987 Jul; 169(7):3062-7.JB

Abstract

The DNA sequence for the promoter region of the Bacillus subtilis citB gene has been determined. Presumed "-10" and "-35" regions of the promoter have been identified, and transcriptional and translational start points of citB have been located. To correlate the DNA sequence of citB with the amino acid sequence of its presumed product, aconitase, it was necessary to devise a scheme for purification of this labile enzyme. This procedure relies on the ability to restore enzyme activity at each stage of purification by incubation in a reducing buffer containing a source of ferrous ions. B. subtilis aconitase appears to be a monomer with a molecular weight of approximately 120,000. The amino-terminal amino acids of aconitase fit the sequence predicted by analysis of the citB gene. Thus, citB codes for aconitase.

Links

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Authors

Dingman DW
No affiliation info available
Sonenshein AL
No affiliation info available

MeSH

Aconitate HydrataseAmino Acid SequenceBacillus subtilisBase SequenceDNA, BacterialGenes, BacterialMolecular WeightPromoter Regions, GeneticProtein BiosynthesisTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3110133

Citation

Dingman, D W., and A L. Sonenshein. "Purification of Aconitase From Bacillus Subtilis and Correlation of Its N-terminal Amino Acid Sequence With the Sequence of the citB Gene." Journal of Bacteriology, vol. 169, no. 7, 1987, pp. 3062-7.
Dingman DW, Sonenshein AL. Purification of aconitase from Bacillus subtilis and correlation of its N-terminal amino acid sequence with the sequence of the citB gene. J Bacteriol. 1987;169(7):3062-7.
Dingman, D. W., & Sonenshein, A. L. (1987). Purification of aconitase from Bacillus subtilis and correlation of its N-terminal amino acid sequence with the sequence of the citB gene. Journal of Bacteriology, 169(7), 3062-7.
Dingman DW, Sonenshein AL. Purification of Aconitase From Bacillus Subtilis and Correlation of Its N-terminal Amino Acid Sequence With the Sequence of the citB Gene. J Bacteriol. 1987;169(7):3062-7. PubMed PMID: 3110133.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification of aconitase from Bacillus subtilis and correlation of its N-terminal amino acid sequence with the sequence of the citB gene. AU - Dingman,D W, AU - Sonenshein,A L, PY - 1987/7/1/pubmed PY - 1987/7/1/medline PY - 1987/7/1/entrez SP - 3062 EP - 7 JF - Journal of bacteriology JO - J Bacteriol VL - 169 IS - 7 N2 - The DNA sequence for the promoter region of the Bacillus subtilis citB gene has been determined. Presumed "-10" and "-35" regions of the promoter have been identified, and transcriptional and translational start points of citB have been located. To correlate the DNA sequence of citB with the amino acid sequence of its presumed product, aconitase, it was necessary to devise a scheme for purification of this labile enzyme. This procedure relies on the ability to restore enzyme activity at each stage of purification by incubation in a reducing buffer containing a source of ferrous ions. B. subtilis aconitase appears to be a monomer with a molecular weight of approximately 120,000. The amino-terminal amino acids of aconitase fit the sequence predicted by analysis of the citB gene. Thus, citB codes for aconitase. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/3110133/Purification_of_aconitase_from_Bacillus_subtilis_and_correlation_of_its_N_terminal_amino_acid_sequence_with_the_sequence_of_the_citB_gene_ DB - PRIME DP - Unbound Medicine ER -