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Auxinic herbicides induce oxidative stress on Cnesterodon decemmaculatus (Pisces: Poeciliidae).
Environ Sci Pollut Res Int. 2019 Jul; 26(20):20485-20498.ES

Abstract

Pesticides might increase the production of reactive oxygen species (ROS). Dicamba (DIC) and 2,4-dichlorophenoxyacetic acid (2,4-D) are auxinic herbicides commonly applied in agroecosystems to control unwanted weeds. We analysed the oxidative damage exerted on the fish Cnesterodon decemmaculatus by an acute exposure to DIC- and 2,4-D-based herbicides formulations Banvel® and DMA®, respectively. The Endo III- and Fpg-modified alkaline comet assay was employed for detecting DNA damage caused by oxidative stress, whereas enzymatic and non-enzymatic biomarkers such as the activities of catalase (CAT), glutathione-S-transferase (GST), acetylcholinesterase (AChE), and glutathione content (GSH) were used to assess antioxidant response to these two herbicides. At the DNA level, results demonstrate that both auxinic herbicides induce oxidative damage at purines level. An increase on CAT and GST activities were detected in 48 h- and 96 h-treated specimens with both auxinics. GSH content decreased in fish exposed to DIC during 48 h and to 2,4-D after 96 h of exposure. Additionally, a diminished AChE activity in specimens treated with DIC and 2,4-D was observed only after 96 h. Total protein content decreased in fish exposed to both auxinics during 96 h. These results represent the first evaluation of oxidative damage related to DIC and 2,4-D exposure on a fish species as the Neotropical freshwater teleost C. decemmaculatus.

Authors+Show Affiliations

Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nro. 3 (esq. 120), B1904AMA, La Plata, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina. Programa de Ecofisiología Aplicada, Instituto de Ecología y Desarrollo Sustentable (PRODEA-INEDES), Universidad Nacional de Lujan, C.C. 221, Luján, Argentina.Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nro. 3 (esq. 120), B1904AMA, La Plata, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nro. 3 (esq. 120), B1904AMA, La Plata, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nro. 3 (esq. 120), B1904AMA, La Plata, Argentina. marecelo.larramendy@gmail.com. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina. marecelo.larramendy@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31102211

Citation

Es Ruiz de Arcaute, Celeste, et al. "Auxinic Herbicides Induce Oxidative Stress On Cnesterodon Decemmaculatus (Pisces: Poeciliidae)." Environmental Science and Pollution Research International, vol. 26, no. 20, 2019, pp. 20485-20498.
Es Ruiz de Arcaute C, Ossana NA, Pérez-Iglesias JM, et al. Auxinic herbicides induce oxidative stress on Cnesterodon decemmaculatus (Pisces: Poeciliidae). Environ Sci Pollut Res Int. 2019;26(20):20485-20498.
Es Ruiz de Arcaute, C., Ossana, N. A., Pérez-Iglesias, J. M., Soloneski, S., & Larramendy, M. L. (2019). Auxinic herbicides induce oxidative stress on Cnesterodon decemmaculatus (Pisces: Poeciliidae). Environmental Science and Pollution Research International, 26(20), 20485-20498. https://doi.org/10.1007/s11356-019-05169-z
Es Ruiz de Arcaute C, et al. Auxinic Herbicides Induce Oxidative Stress On Cnesterodon Decemmaculatus (Pisces: Poeciliidae). Environ Sci Pollut Res Int. 2019;26(20):20485-20498. PubMed PMID: 31102211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Auxinic herbicides induce oxidative stress on Cnesterodon decemmaculatus (Pisces: Poeciliidae). AU - Es Ruiz de Arcaute,Celeste, AU - Ossana,Natalia A, AU - Pérez-Iglesias,Juan Manuel, AU - Soloneski,Sonia, AU - Larramendy,Marcelo L, Y1 - 2019/05/17/ PY - 2018/12/20/received PY - 2019/04/11/accepted PY - 2019/5/19/pubmed PY - 2019/9/17/medline PY - 2019/5/19/entrez KW - Acetylcholinesterase KW - Catalase KW - Endo III- and Fpg-modified comet assay KW - Glutathione KW - Glutathione-S-transferase SP - 20485 EP - 20498 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 26 IS - 20 N2 - Pesticides might increase the production of reactive oxygen species (ROS). Dicamba (DIC) and 2,4-dichlorophenoxyacetic acid (2,4-D) are auxinic herbicides commonly applied in agroecosystems to control unwanted weeds. We analysed the oxidative damage exerted on the fish Cnesterodon decemmaculatus by an acute exposure to DIC- and 2,4-D-based herbicides formulations Banvel® and DMA®, respectively. The Endo III- and Fpg-modified alkaline comet assay was employed for detecting DNA damage caused by oxidative stress, whereas enzymatic and non-enzymatic biomarkers such as the activities of catalase (CAT), glutathione-S-transferase (GST), acetylcholinesterase (AChE), and glutathione content (GSH) were used to assess antioxidant response to these two herbicides. At the DNA level, results demonstrate that both auxinic herbicides induce oxidative damage at purines level. An increase on CAT and GST activities were detected in 48 h- and 96 h-treated specimens with both auxinics. GSH content decreased in fish exposed to DIC during 48 h and to 2,4-D after 96 h of exposure. Additionally, a diminished AChE activity in specimens treated with DIC and 2,4-D was observed only after 96 h. Total protein content decreased in fish exposed to both auxinics during 96 h. These results represent the first evaluation of oxidative damage related to DIC and 2,4-D exposure on a fish species as the Neotropical freshwater teleost C. decemmaculatus. SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/31102211/Auxinic_herbicides_induce_oxidative_stress_on_Cnesterodon_decemmaculatus__Pisces:_Poeciliidae__ L2 - https://dx.doi.org/10.1007/s11356-019-05169-z DB - PRIME DP - Unbound Medicine ER -