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Serologically defined V region subgroups of human lambda light chains.
J Immunol. 1987 Aug 01; 139(3):824-30.JI

Abstract

The availability of numerous antisera prepared against lambda-type Bence Jones proteins and lambda chains of known amino acid sequence has led to the differentiation and classification of human lambda light chains into one of five V lambda subgroups. The five serologically defined subgroups, V lambda I, V lambda II, V lambda III, V lambda IV, and V lambda VI, correspond to the chemical classification that is based on sequence homologies in the first framework region (FR1). Proteins designated by sequence as lambda V react with specific anti-lambda II antisera and are thus included in the V lambda II subgroup classification. The isotypic nature of the five V lambda subgroups was evidenced through analyses of lambda-type light chains that were isolated from the IgG of normal individuals. Based on analyses of 116 Bence Jones proteins, the frequency of distribution of the lambda I, lambda II/V, lambda III, lambda IV, and lambda VI proteins in the normal lambda chain population is estimated to be 27%, 37%, 23%, 3%, and 10%, respectively. This distribution of V lambda subgroups was comparable to that found among 82 monoclonal Ig lambda proteins. Considerable V lambda intragroup antigenic heterogeneity was also apparent. At least two sub-subgroups were identified among each of the five major V lambda subgroups, implying the existence of multiple genes in the human V lambda genome. The V lambda classification of 54 Ig lambda proteins obtained from patients with primary or multiple myeloma-associated amyloidosis substantiated the preferential association of lambda VI light chains with amyloidosis AL and the predominance of the normally rare V lambda VI subgroup in this disease.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3110284

Citation

Solomon, A, and D T. Weiss. "Serologically Defined V Region Subgroups of Human Lambda Light Chains." Journal of Immunology (Baltimore, Md. : 1950), vol. 139, no. 3, 1987, pp. 824-30.
Solomon A, Weiss DT. Serologically defined V region subgroups of human lambda light chains. J Immunol. 1987;139(3):824-30.
Solomon, A., & Weiss, D. T. (1987). Serologically defined V region subgroups of human lambda light chains. Journal of Immunology (Baltimore, Md. : 1950), 139(3), 824-30.
Solomon A, Weiss DT. Serologically Defined V Region Subgroups of Human Lambda Light Chains. J Immunol. 1987 Aug 1;139(3):824-30. PubMed PMID: 3110284.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serologically defined V region subgroups of human lambda light chains. AU - Solomon,A, AU - Weiss,D T, PY - 1987/8/1/pubmed PY - 1987/8/1/medline PY - 1987/8/1/entrez SP - 824 EP - 30 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 139 IS - 3 N2 - The availability of numerous antisera prepared against lambda-type Bence Jones proteins and lambda chains of known amino acid sequence has led to the differentiation and classification of human lambda light chains into one of five V lambda subgroups. The five serologically defined subgroups, V lambda I, V lambda II, V lambda III, V lambda IV, and V lambda VI, correspond to the chemical classification that is based on sequence homologies in the first framework region (FR1). Proteins designated by sequence as lambda V react with specific anti-lambda II antisera and are thus included in the V lambda II subgroup classification. The isotypic nature of the five V lambda subgroups was evidenced through analyses of lambda-type light chains that were isolated from the IgG of normal individuals. Based on analyses of 116 Bence Jones proteins, the frequency of distribution of the lambda I, lambda II/V, lambda III, lambda IV, and lambda VI proteins in the normal lambda chain population is estimated to be 27%, 37%, 23%, 3%, and 10%, respectively. This distribution of V lambda subgroups was comparable to that found among 82 monoclonal Ig lambda proteins. Considerable V lambda intragroup antigenic heterogeneity was also apparent. At least two sub-subgroups were identified among each of the five major V lambda subgroups, implying the existence of multiple genes in the human V lambda genome. The V lambda classification of 54 Ig lambda proteins obtained from patients with primary or multiple myeloma-associated amyloidosis substantiated the preferential association of lambda VI light chains with amyloidosis AL and the predominance of the normally rare V lambda VI subgroup in this disease. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/3110284/Serologically_defined_V_region_subgroups_of_human_lambda_light_chains_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=3110284 DB - PRIME DP - Unbound Medicine ER -