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Phthalate induced oxidative stress and DNA damage in earthworms (Eisenia fetida).
Environ Int. 2019 08; 129:10-17.EI

Abstract

Phthalates (phthalic acid esters) have been widely applied as plasticizers. They are ubiquitous contaminants in soils, thereby posing a threat to human health. In this study, ecotoxicological effects of three typical PAEs (dimethyl phthalate-DMP, di-n-octyl phthalate-DOP and butyl benzyl phthalate-BBP) were investigated. As a biological indicator, earthworms (Eisenia fetida) were exposed to phthalates at various doses (0, 0.1, 1, 10 and 50 mg/kg) for different times (7, 14, 21, and 28 d). We evaluated the effects of phthalates on reactive oxygen species (ROS) generation, antioxidant enzymes (superoxide dismutase-SOD, peroxidase-POD and catalase-CAT) activities, glutathione S-transferase enzyme (GST) activity, malondialdehyde (MDA) content and DNA damage. Results showed that ROS content increased with increasing phthalates, whereas ROS content generally increased and then decreased with exposure time. However, antioxidant enzymes activities in earthworms displayed different trends. The GST activity in high-dose treatment group was significantly activated. For DMP and DOP, lipid peroxidation mainly occurred between 14 and 28 d, while for BBP, it primarily existed after 7 d and then disappeared after 28 d. Besides, comet assay indicated that there was a dose-response relationship between the DNA damage and phthalate dose, following DMP > DOP > BBP. Given their toxicity, it is important to understand the mechanisms associated with their eco-toxicity and to reduce their adverse impacts on the environment.

Authors+Show Affiliations

College of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai'an 271000, PR China.College of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai'an 271000, PR China.College of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai'an 271000, PR China.College of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai'an 271000, PR China.College of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai'an 271000, PR China.College of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai'an 271000, PR China.College of Resources and Environment, Key Laboratory of Agricultural Environment, Shandong Agricultural University, Tai'an 271000, PR China. Electronic address: jwang@sdau.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31102950

Citation

Song, Peipei, et al. "Phthalate Induced Oxidative Stress and DNA Damage in Earthworms (Eisenia Fetida)." Environment International, vol. 129, 2019, pp. 10-17.
Song P, Gao J, Li X, et al. Phthalate induced oxidative stress and DNA damage in earthworms (Eisenia fetida). Environ Int. 2019;129:10-17.
Song, P., Gao, J., Li, X., Zhang, C., Zhu, L., Wang, J., & Wang, J. (2019). Phthalate induced oxidative stress and DNA damage in earthworms (Eisenia fetida). Environment International, 129, 10-17. https://doi.org/10.1016/j.envint.2019.04.074
Song P, et al. Phthalate Induced Oxidative Stress and DNA Damage in Earthworms (Eisenia Fetida). Environ Int. 2019;129:10-17. PubMed PMID: 31102950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phthalate induced oxidative stress and DNA damage in earthworms (Eisenia fetida). AU - Song,Peipei, AU - Gao,Jianpeng, AU - Li,Xianxu, AU - Zhang,Cui, AU - Zhu,Lusheng, AU - Wang,Jinhua, AU - Wang,Jun, Y1 - 2019/05/15/ PY - 2019/03/31/received PY - 2019/04/26/revised PY - 2019/04/30/accepted PY - 2019/5/19/pubmed PY - 2019/11/8/medline PY - 2019/5/19/entrez KW - Antioxidant enzymes KW - DNA damage KW - Lipid peroxidation KW - Phthalic acid esters KW - ROS SP - 10 EP - 17 JF - Environment international JO - Environ Int VL - 129 N2 - Phthalates (phthalic acid esters) have been widely applied as plasticizers. They are ubiquitous contaminants in soils, thereby posing a threat to human health. In this study, ecotoxicological effects of three typical PAEs (dimethyl phthalate-DMP, di-n-octyl phthalate-DOP and butyl benzyl phthalate-BBP) were investigated. As a biological indicator, earthworms (Eisenia fetida) were exposed to phthalates at various doses (0, 0.1, 1, 10 and 50 mg/kg) for different times (7, 14, 21, and 28 d). We evaluated the effects of phthalates on reactive oxygen species (ROS) generation, antioxidant enzymes (superoxide dismutase-SOD, peroxidase-POD and catalase-CAT) activities, glutathione S-transferase enzyme (GST) activity, malondialdehyde (MDA) content and DNA damage. Results showed that ROS content increased with increasing phthalates, whereas ROS content generally increased and then decreased with exposure time. However, antioxidant enzymes activities in earthworms displayed different trends. The GST activity in high-dose treatment group was significantly activated. For DMP and DOP, lipid peroxidation mainly occurred between 14 and 28 d, while for BBP, it primarily existed after 7 d and then disappeared after 28 d. Besides, comet assay indicated that there was a dose-response relationship between the DNA damage and phthalate dose, following DMP > DOP > BBP. Given their toxicity, it is important to understand the mechanisms associated with their eco-toxicity and to reduce their adverse impacts on the environment. SN - 1873-6750 UR - https://www.unboundmedicine.com/medline/citation/31102950/Phthalate_induced_oxidative_stress_and_DNA_damage_in_earthworms__Eisenia_fetida__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0160-4120(19)31073-6 DB - PRIME DP - Unbound Medicine ER -