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Comparing migraine with and without aura to healthy controls using RNA sequencing.

Abstract

BACKGROUND

Migraine mechanisms are *These authors contributed equally to this work. only partly known. Some studies have previously described genes differentially expressed between blood from migraineurs and controls. The objective of this study was to describe gene expression in subtypes of migraine outside of attack and in healthy controls.

METHODS

We extensively phenotyped 17 migraine without aura and nine migraine with aura female patients, and 20 age-matched female controls. Cubital venous blood was RNA sequenced. Genes differentially expressed between migraineurs (migraine without aura and migraine with aura) and controls, and between migraine without aura and migraine with aura were identified using a case-control design. A co-expression network was constructed to investigate the difference between migraineurs and healthy controls at the network level.

RESULTS

We found two differentially expressed genes: NMNAT2 and RETN. Both were differentially expressed between migraine with aura and controls, but they could not be replicated in an independent cohort. Co-expression network analysis resulted in one cluster of highly interconnected genes that was nominally significantly associated with migraine; however, no pathways or gene ontology terms were detected.

CONCLUSIONS

We showed no clear distinct difference in gene expression profiles of peripheral blood of migraineurs and controls and were not able to replicate findings from previous studies. A larger sample size may be needed to detect minor differences.

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  • Authors+Show Affiliations

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    1 Danish Headache Center, Department of Neurology, Rigshospitalet Glostrup, Glostrup, Denmark.

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    1 Danish Headache Center, Department of Neurology, Rigshospitalet Glostrup, Glostrup, Denmark.

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    2 deCODE Genetics, Reykjavik, Iceland.

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    1 Danish Headache Center, Department of Neurology, Rigshospitalet Glostrup, Glostrup, Denmark.

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    1 Danish Headache Center, Department of Neurology, Rigshospitalet Glostrup, Glostrup, Denmark.

    ,

    2 deCODE Genetics, Reykjavik, Iceland. 3 Institute for Biological Psychiatry, Mental Health Center Sankt Hans, Denmark.

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    2 deCODE Genetics, Reykjavik, Iceland.

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    2 deCODE Genetics, Reykjavik, Iceland.

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    1 Danish Headache Center, Department of Neurology, Rigshospitalet Glostrup, Glostrup, Denmark. 3 Institute for Biological Psychiatry, Mental Health Center Sankt Hans, Denmark. 4 Novo Nordic Foundation Centre for Protein Research, Copenhagen University, Copenhagen, Denmark.

    1 Danish Headache Center, Department of Neurology, Rigshospitalet Glostrup, Glostrup, Denmark.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31104508

    Citation

    Kogelman, Lisette Ja, et al. "Comparing Migraine With and Without Aura to Healthy Controls Using RNA Sequencing." Cephalalgia : an International Journal of Headache, 2019, p. 333102419851812.
    Kogelman LJ, Falkenberg K, Halldorsson GH, et al. Comparing migraine with and without aura to healthy controls using RNA sequencing. Cephalalgia. 2019.
    Kogelman, L. J., Falkenberg, K., Halldorsson, G. H., Poulsen, L. U., Worm, J., Ingason, A., ... Olesen, J. (2019). Comparing migraine with and without aura to healthy controls using RNA sequencing. Cephalalgia : an International Journal of Headache, p. 333102419851812. doi:10.1177/0333102419851812.
    Kogelman LJ, et al. Comparing Migraine With and Without Aura to Healthy Controls Using RNA Sequencing. Cephalalgia. 2019 May 19;333102419851812. PubMed PMID: 31104508.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Comparing migraine with and without aura to healthy controls using RNA sequencing. AU - Kogelman,Lisette Ja, AU - Falkenberg,Katrine, AU - Halldorsson,Gisli H, AU - Poulsen,Lau U, AU - Worm,Jacob, AU - Ingason,Andres, AU - Stefansson,Hreinn, AU - Stefansson,Kari, AU - Hansen,Thomas F, AU - Olesen,Jes, Y1 - 2019/05/19/ PY - 2019/5/21/entrez KW - Migraine KW - aura KW - co-expression network KW - gene expression KW - whole blood SP - 333102419851812 EP - 333102419851812 JF - Cephalalgia : an international journal of headache JO - Cephalalgia N2 - BACKGROUND: Migraine mechanisms are *These authors contributed equally to this work. only partly known. Some studies have previously described genes differentially expressed between blood from migraineurs and controls. The objective of this study was to describe gene expression in subtypes of migraine outside of attack and in healthy controls. METHODS: We extensively phenotyped 17 migraine without aura and nine migraine with aura female patients, and 20 age-matched female controls. Cubital venous blood was RNA sequenced. Genes differentially expressed between migraineurs (migraine without aura and migraine with aura) and controls, and between migraine without aura and migraine with aura were identified using a case-control design. A co-expression network was constructed to investigate the difference between migraineurs and healthy controls at the network level. RESULTS: We found two differentially expressed genes: NMNAT2 and RETN. Both were differentially expressed between migraine with aura and controls, but they could not be replicated in an independent cohort. Co-expression network analysis resulted in one cluster of highly interconnected genes that was nominally significantly associated with migraine; however, no pathways or gene ontology terms were detected. CONCLUSIONS: We showed no clear distinct difference in gene expression profiles of peripheral blood of migraineurs and controls and were not able to replicate findings from previous studies. A larger sample size may be needed to detect minor differences. SN - 1468-2982 UR - https://www.unboundmedicine.com/medline/citation/31104508/Comparing_migraine_with_and_without_aura_to_healthy_controls_using_RNA_sequencing L2 - http://journals.sagepub.com/doi/full/10.1177/0333102419851812?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -