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High-Density Mapping of Triple Rust Resistance in Barley Using DArT-Seq Markers.
Front Plant Sci. 2019; 10:467.FP

Abstract

The recent availability of an assembled and annotated genome reference sequence for the diploid crop barley (Hordeum vulgare L.) provides new opportunities to study the genetic basis of agronomically important traits such as resistance to stripe [Puccinia striiformis f. sp. hordei (Psh)], leaf [P. hordei (Ph)], and stem [P. graminis f. sp. tritici (Pgt)] rust diseases. The European barley cultivar Pompadour is known to possess high levels of resistance to leaf rust, predominantly due to adult plant resistance (APR) gene Rph20. We developed a barley recombinant inbred line (RIL) population from a cross between Pompadour and the leaf rust and stripe rust susceptible selection Biosaline-19 (B-19), and genotyped this population using DArT-Seq genotyping by sequencing (GBS) markers. In the current study, we produced a high-density linkage map comprising 8,610 (SNP and in silico) markers spanning 5957.6 cM, with the aim of mapping loci for resistance to leaf rust, stem rust, and stripe rust. The RIL population was phenotyped in the field with Psh (Mexico and Ecuador) and Ph (Australia) and in the greenhouse at the seedling stage with Australian Ph and Pgt races, and at Wageningen University with a European variant of Psh race 24 (PshWUR). For Psh, we identified a consistent field QTL on chromosome 2H across all South American field sites and years. Two complementary resistance genes were mapped to chromosomes 1H and 4H at the seedling stage in response to PshWUR, likely to be the loci rpsEm1 and rpsEm2 previously reported from the cultivar Emir from which Pompadour was bred. For leaf rust, we determined that Rph20 in addition to two minor-effect QTL on 1H and 3H were effective at the seedling stage, whilst seedling resistance to stem rust was due to QTL on chromosomes 3H and 7H conferred by Pompadour and B-19, respectively.

Authors+Show Affiliations

Plant Breeding Institute Cobbitty, Sydney Institute of Agriculture, The University of Sydney, Sydney, NSW, Australia.Plant Breeding Institute Cobbitty, Sydney Institute of Agriculture, The University of Sydney, Sydney, NSW, Australia.International Maize and Wheat Improvement Center, Texcoco, Mexico. Campo Experimental Valle de México, INIFAP, Chapingo, Mexico.International Maize and Wheat Improvement Center, Texcoco, Mexico. Campo Experimental Valle de México, INIFAP, Chapingo, Mexico.Instituto Nacional de Investigaciones Agropecuarias (INIAP), Quito, Ecuador.Agriculture Victoria, AgriBio, Centre for AgriBioscience, La Trobe University, Melbourne, VIC, Australia.Agriculture Victoria, AgriBio, Centre for AgriBioscience, La Trobe University, Melbourne, VIC, Australia.Plant Breeding, Wageningen University & Research, Wageningen, Netherlands.Plant Breeding Institute Cobbitty, Sydney Institute of Agriculture, The University of Sydney, Sydney, NSW, Australia.Plant Breeding Institute Cobbitty, Sydney Institute of Agriculture, The University of Sydney, Sydney, NSW, Australia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31105717

Citation

Dracatos, Peter M., et al. "High-Density Mapping of Triple Rust Resistance in Barley Using DArT-Seq Markers." Frontiers in Plant Science, vol. 10, 2019, p. 467.
Dracatos PM, Haghdoust R, Singh RP, et al. High-Density Mapping of Triple Rust Resistance in Barley Using DArT-Seq Markers. Front Plant Sci. 2019;10:467.
Dracatos, P. M., Haghdoust, R., Singh, R. P., Huerta Espino, J., Barnes, C. W., Forrest, K., Hayden, M., Niks, R. E., Park, R. F., & Singh, D. (2019). High-Density Mapping of Triple Rust Resistance in Barley Using DArT-Seq Markers. Frontiers in Plant Science, 10, 467. https://doi.org/10.3389/fpls.2019.00467
Dracatos PM, et al. High-Density Mapping of Triple Rust Resistance in Barley Using DArT-Seq Markers. Front Plant Sci. 2019;10:467. PubMed PMID: 31105717.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-Density Mapping of Triple Rust Resistance in Barley Using DArT-Seq Markers. AU - Dracatos,Peter M, AU - Haghdoust,Rouja, AU - Singh,Ravi P, AU - Huerta Espino,Julio, AU - Barnes,Charles W, AU - Forrest,Kerrie, AU - Hayden,Matthew, AU - Niks,Rients E, AU - Park,Robert F, AU - Singh,Davinder, Y1 - 2019/04/26/ PY - 2019/01/30/received PY - 2019/03/28/accepted PY - 2019/5/21/entrez PY - 2019/5/21/pubmed PY - 2019/5/21/medline KW - DArT-Seq markers KW - QTL KW - barley KW - high-density linkage map KW - rust resistance SP - 467 EP - 467 JF - Frontiers in plant science JO - Front Plant Sci VL - 10 N2 - The recent availability of an assembled and annotated genome reference sequence for the diploid crop barley (Hordeum vulgare L.) provides new opportunities to study the genetic basis of agronomically important traits such as resistance to stripe [Puccinia striiformis f. sp. hordei (Psh)], leaf [P. hordei (Ph)], and stem [P. graminis f. sp. tritici (Pgt)] rust diseases. The European barley cultivar Pompadour is known to possess high levels of resistance to leaf rust, predominantly due to adult plant resistance (APR) gene Rph20. We developed a barley recombinant inbred line (RIL) population from a cross between Pompadour and the leaf rust and stripe rust susceptible selection Biosaline-19 (B-19), and genotyped this population using DArT-Seq genotyping by sequencing (GBS) markers. In the current study, we produced a high-density linkage map comprising 8,610 (SNP and in silico) markers spanning 5957.6 cM, with the aim of mapping loci for resistance to leaf rust, stem rust, and stripe rust. The RIL population was phenotyped in the field with Psh (Mexico and Ecuador) and Ph (Australia) and in the greenhouse at the seedling stage with Australian Ph and Pgt races, and at Wageningen University with a European variant of Psh race 24 (PshWUR). For Psh, we identified a consistent field QTL on chromosome 2H across all South American field sites and years. Two complementary resistance genes were mapped to chromosomes 1H and 4H at the seedling stage in response to PshWUR, likely to be the loci rpsEm1 and rpsEm2 previously reported from the cultivar Emir from which Pompadour was bred. For leaf rust, we determined that Rph20 in addition to two minor-effect QTL on 1H and 3H were effective at the seedling stage, whilst seedling resistance to stem rust was due to QTL on chromosomes 3H and 7H conferred by Pompadour and B-19, respectively. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/31105717/High_Density_Mapping_of_Triple_Rust_Resistance_in_Barley_Using_DArT_Seq_Markers_ L2 - https://doi.org/10.3389/fpls.2019.00467 DB - PRIME DP - Unbound Medicine ER -
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