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CHID1 positively regulates RLR antiviral signaling by targeting the RIG-I/VISA signalosome.
J Med Virol 2019; 91(9):1668-1678JM

Abstract

Retinoic acid-inducible gene-I (RIG-I) belongs to the RIGI-like receptors (RLRs), a class of primary pattern recognition receptors. It senses viral double-strand RNA in the cytoplasm and delivers the activated signal to its adaptor virus-induced signaling adapter (VISA), which then recruits the downstream TNF receptor-associated factors and kinases, triggering a downstream signal cascade that leads to the production of proinflammatory cytokines and antiviral interferons (IFNs). However, the mechanism of RIG-I-mediated antiviral signaling is not fully understood. Here, we demonstrate that chitinase domain-containing 1 (CHID1), a member of the chitinase family, positively regulates the RLR antiviral signaling pathway by targeting the RIG-I/VISA signalosome. CHID1 overexpression enhances the activation of nuclear factor κB (NF-кB) and interferon regulatory factor 3 (IRF3) triggered by Sendai virus (SeV) by promoting the polyubiquitination of RIG-I and VISA, thereby potentiating IFN-β production. CHID1 knockdown in human 239T cells inhibits SeV-induced activation of IRF3 and NF-κB and the induction of IFN-β. These results indicate that CHID1 positively regulates RLR antiviral signal, revealing the novel mechanism of the RIG-I antiviral signaling pathway.

Authors+Show Affiliations

Key Laboratory of Functional Small Organic Molecules, Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi, China.Key Laboratory of Functional Small Organic Molecules, Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi, China.Key Laboratory of Functional Small Organic Molecules, Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi, China.Key Laboratory of Functional Small Organic Molecules, Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi, China.Key Laboratory of Functional Small Organic Molecules, Ministry of Education and College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31106867

Citation

Li, Sheng-Na, et al. "CHID1 Positively Regulates RLR Antiviral Signaling By Targeting the RIG-I/VISA Signalosome." Journal of Medical Virology, vol. 91, no. 9, 2019, pp. 1668-1678.
Li SN, Ling T, Yang YX, et al. CHID1 positively regulates RLR antiviral signaling by targeting the RIG-I/VISA signalosome. J Med Virol. 2019;91(9):1668-1678.
Li, S. N., Ling, T., Yang, Y. X., Huang, J. P., & Xu, L. G. (2019). CHID1 positively regulates RLR antiviral signaling by targeting the RIG-I/VISA signalosome. Journal of Medical Virology, 91(9), pp. 1668-1678. doi:10.1002/jmv.25508.
Li SN, et al. CHID1 Positively Regulates RLR Antiviral Signaling By Targeting the RIG-I/VISA Signalosome. J Med Virol. 2019;91(9):1668-1678. PubMed PMID: 31106867.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CHID1 positively regulates RLR antiviral signaling by targeting the RIG-I/VISA signalosome. AU - Li,Sheng-Na, AU - Ling,Ting, AU - Yang,Ya-Xian, AU - Huang,Jing-Ping, AU - Xu,Liang-Guo, Y1 - 2019/06/25/ PY - 2019/02/25/received PY - 2019/05/09/revised PY - 2019/05/09/accepted PY - 2019/5/21/pubmed PY - 2019/5/21/medline PY - 2019/5/21/entrez KW - CHID1 KW - RIG-I KW - VISA KW - ubiquitination SP - 1668 EP - 1678 JF - Journal of medical virology JO - J. Med. Virol. VL - 91 IS - 9 N2 - Retinoic acid-inducible gene-I (RIG-I) belongs to the RIGI-like receptors (RLRs), a class of primary pattern recognition receptors. It senses viral double-strand RNA in the cytoplasm and delivers the activated signal to its adaptor virus-induced signaling adapter (VISA), which then recruits the downstream TNF receptor-associated factors and kinases, triggering a downstream signal cascade that leads to the production of proinflammatory cytokines and antiviral interferons (IFNs). However, the mechanism of RIG-I-mediated antiviral signaling is not fully understood. Here, we demonstrate that chitinase domain-containing 1 (CHID1), a member of the chitinase family, positively regulates the RLR antiviral signaling pathway by targeting the RIG-I/VISA signalosome. CHID1 overexpression enhances the activation of nuclear factor κB (NF-кB) and interferon regulatory factor 3 (IRF3) triggered by Sendai virus (SeV) by promoting the polyubiquitination of RIG-I and VISA, thereby potentiating IFN-β production. CHID1 knockdown in human 239T cells inhibits SeV-induced activation of IRF3 and NF-κB and the induction of IFN-β. These results indicate that CHID1 positively regulates RLR antiviral signal, revealing the novel mechanism of the RIG-I antiviral signaling pathway. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/31106867/CHID1_positively_regulates_RLR_antiviral_signaling_by_targeting_the_RIG-I/VISA_signalosome L2 - https://doi.org/10.1002/jmv.25508 DB - PRIME DP - Unbound Medicine ER -