Target-Guided Isolation of Three Main Antioxidants from Mahonia bealei (Fort.) Carr. Leaves Using HSCCC.
Molecules. 2019 May 17; 24(10)M

Abstract

Mahonia bealei (Fort.) Carr. is an economically important plant that is widely cultivated in Southwest China. Its leaves are commonly used for tea and contain an abundance of antioxidant compounds. However, methods of the systematic purification of antioxidants from M. bealei are lacking. In this study, antioxidants from this plant were effectively and rapidly enriched. First, antioxidants were screened using online 1,1-diphenyl-2-picryl-hydrazyl radical (DPPH)-high performance liquid chromatography (HPLC), followed by separation using high-speed countercurrent chromatography with an optical solvent system composed of n-hexane/ethyl acetate/methanol/water (1:5:1:5, v/v/v/v). Three phenolics-chlorogenic acid (1, 8.3 mg), quercetin-3-O-β-d-glucopyranoside (2, 20.5 mg), and isorhamnetin-3-O-β-d-glucopyranoside (3, 28.4 mg)-were obtained from the ethyl acetate-soluble fraction (240 mg) by one-step separation. The chemical structures of the phenolics were characterized by MS and NMR techniques, and the purity of each compound was >92.0% as determined by HPLC. The isolated compounds were assessed by scavenging activities on DPPH and superoxide radicals as well as cytoprotective assays, all of which showed similar trends regarding the antioxidant capacities of the compounds. Moreover, compounds 1-3 significantly attenuated the lipid peroxidation and antioxidant enzyme activities in H2O2-treated RAW264.7 cells. Our study demonstrated the efficiency of a newly developed integrative system for the comprehensive characterization of pure compounds from M. bealei, which will allow their use as reference substances.

Links

PMC Free PDF

Authors+Show Affiliations

Hu W

Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental protection/Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian 223300, China. hu_weicheng@163.com.

Zhou J

Shen T

Wang X

MeSH

AnimalsAntioxidantsChromatography, High Pressure LiquidHydrogen PeroxideLipid PeroxidationMahoniaMiceMolecular StructureOxidative StressPhenolsPlant ExtractsPlant LeavesRAW 264.7 Cells

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31108973

Citation

Hu, Weicheng, et al. "Target-Guided Isolation of Three Main Antioxidants From Mahonia Bealei (Fort.) Carr. Leaves Using HSCCC." Molecules (Basel, Switzerland), vol. 24, no. 10, 2019.

Hu W, Zhou J, Shen T, et al. Target-Guided Isolation of Three Main Antioxidants from Mahonia bealei (Fort.) Carr. Leaves Using HSCCC. Molecules. 2019;24(10).

Hu, W., Zhou, J., Shen, T., & Wang, X. (2019). Target-Guided Isolation of Three Main Antioxidants from Mahonia bealei (Fort.) Carr. Leaves Using HSCCC. Molecules (Basel, Switzerland), 24(10). https://doi.org/10.3390/molecules24101907

Hu W, et al. Target-Guided Isolation of Three Main Antioxidants From Mahonia Bealei (Fort.) Carr. Leaves Using HSCCC. Molecules. 2019 May 17;24(10) PubMed PMID: 31108973.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Target-Guided Isolation of Three Main Antioxidants from Mahonia bealei (Fort.) Carr. Leaves Using HSCCC. AU - Hu,Weicheng, AU - Zhou,Jing, AU - Shen,Ting, AU - Wang,Xinfeng, Y1 - 2019/05/17/ PY - 2019/05/01/received PY - 2019/05/14/revised PY - 2019/05/15/accepted PY - 2019/5/22/entrez PY - 2019/5/22/pubmed PY - 2019/11/16/medline KW - DPPH–HPLC KW - HSCCC KW - Mahonia bealei (Fort.) Carr. KW - antioxidant activity JF - Molecules (Basel, Switzerland) JO - Molecules VL - 24 IS - 10 N2 - Mahonia bealei (Fort.) Carr. is an economically important plant that is widely cultivated in Southwest China. Its leaves are commonly used for tea and contain an abundance of antioxidant compounds. However, methods of the systematic purification of antioxidants from M. bealei are lacking. In this study, antioxidants from this plant were effectively and rapidly enriched. First, antioxidants were screened using online 1,1-diphenyl-2-picryl-hydrazyl radical (DPPH)-high performance liquid chromatography (HPLC), followed by separation using high-speed countercurrent chromatography with an optical solvent system composed of n-hexane/ethyl acetate/methanol/water (1:5:1:5, v/v/v/v). Three phenolics-chlorogenic acid (1, 8.3 mg), quercetin-3-O-β-d-glucopyranoside (2, 20.5 mg), and isorhamnetin-3-O-β-d-glucopyranoside (3, 28.4 mg)-were obtained from the ethyl acetate-soluble fraction (240 mg) by one-step separation. The chemical structures of the phenolics were characterized by MS and NMR techniques, and the purity of each compound was >92.0% as determined by HPLC. The isolated compounds were assessed by scavenging activities on DPPH and superoxide radicals as well as cytoprotective assays, all of which showed similar trends regarding the antioxidant capacities of the compounds. Moreover, compounds 1-3 significantly attenuated the lipid peroxidation and antioxidant enzyme activities in H2O2-treated RAW264.7 cells. Our study demonstrated the efficiency of a newly developed integrative system for the comprehensive characterization of pure compounds from M. bealei, which will allow their use as reference substances. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/31108973/Target_Guided_Isolation_of_Three_Main_Antioxidants_from_Mahonia_bealei__Fort___Carr__Leaves_Using_HSCCC_ DB - PRIME DP - Unbound Medicine ER -

Tags

Target-Guided Isolation of Three Main Antioxidants from Mahonia bealei (Fort.) Carr. Leaves Using HSCCC.Molecules. 2019 May 17; 24(10)M