Tags

Type your tag names separated by a space and hit enter

Serum siderocalin levels in patients with tuberculosis and HIV infection.

Abstract

OBJECTIVES

Mycobacterium tuberculosis produces high-affinity siderophores that play essential roles in iron acquisition and tuberculosis (TB) pathogenesis. In response, host cells secrete a siderophore-binding protein, siderocalin, to limit the bacteria's access to iron. The objective of the present study was to evaluate the levels of siderocalin in patients with TB with or without HIV infection compared to controls.

METHODS

Siderocalin levels were tested using a neutrophil gelatinase-associated lipocalin (NGAL) ELISA kit in four populations: HIV-infected patients with TB (HIVpos, TBpos), non-HIV-infected patients with TB (HIVneg, TBpos), HIV-infected patients without TB (HIVpos, TBneg), and healthy controls (HIVneg, TBneg).

RESULTS

Serum siderocalin levels were significantly elevated in patients with TB regardless of their HIV status (HIVneg, TBpos 920 (480-1050) pg/ml; HIVpos, TBpos 494 (166-1050) pg/ml), whereas lower levels of siderocalin were seen in HIV-positive patients (HIVpos, TBneg 268 (77-937) pg/ml; HIVneg, TBneg 453 (193-994) pg/ml).

CONCLUSIONS

The results indicate that active TB leads to an up-regulation of serum siderocalin regardless of HIV status, whereas HIV infection leads to a down-regulation of serum siderocalin levels in both TB-negative and TB-positive individuals. Further studies are needed to evaluate siderocalin as a potential marker of active TB and to clarify its role in the pathogenesis of HIV-associated TB.

Links

  • FREE Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Infectious Diseases, Christian Medical College, Vellore, India. Electronic address: georgemvarghese@hotmail.com.

    ,

    Department of Medicine, Christian Medical College, Vellore, India.

    ,

    Department of Infectious Diseases, Christian Medical College, Vellore, India.

    ,

    Department of Infectious Diseases, Christian Medical College, Vellore, India.

    ,

    Department of Haematology, Christian Medical College, Vellore, India.

    ,

    Department of Nephrology, Christian Medical College, Vellore, India.

    Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, USA.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31112763

    Citation

    Varghese, George M., et al. "Serum Siderocalin Levels in Patients With Tuberculosis and HIV Infection." International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, vol. 85, 2019, pp. 132-134.
    Varghese GM, Turaka VP, Janardhanan J, et al. Serum siderocalin levels in patients with tuberculosis and HIV infection. Int J Infect Dis. 2019;85:132-134.
    Varghese, G. M., Turaka, V. P., Janardhanan, J., Yadav, S., Lakshmi, K. M., S, V. T., & Cherayil, B. (2019). Serum siderocalin levels in patients with tuberculosis and HIV infection. International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, 85, pp. 132-134. doi:10.1016/j.ijid.2019.05.020.
    Varghese GM, et al. Serum Siderocalin Levels in Patients With Tuberculosis and HIV Infection. Int J Infect Dis. 2019 May 18;85:132-134. PubMed PMID: 31112763.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Serum siderocalin levels in patients with tuberculosis and HIV infection. AU - Varghese,George M, AU - Turaka,Vijay Prakash, AU - Janardhanan,Jeshina, AU - Yadav,Sadhana, AU - Lakshmi,Kavitha M, AU - S,Vijayakumar T, AU - Cherayil,Bobby, Y1 - 2019/05/18/ PY - 2019/04/18/received PY - 2019/05/09/revised PY - 2019/05/10/accepted PY - 2019/5/22/pubmed PY - 2019/5/22/medline PY - 2019/5/22/entrez KW - HIV KW - Iron KW - Mycobacterium tuberculosis KW - Siderocalin KW - Siderophore KW - Tuberculosis SP - 132 EP - 134 JF - International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases JO - Int. J. Infect. Dis. VL - 85 N2 - OBJECTIVES: Mycobacterium tuberculosis produces high-affinity siderophores that play essential roles in iron acquisition and tuberculosis (TB) pathogenesis. In response, host cells secrete a siderophore-binding protein, siderocalin, to limit the bacteria's access to iron. The objective of the present study was to evaluate the levels of siderocalin in patients with TB with or without HIV infection compared to controls. METHODS: Siderocalin levels were tested using a neutrophil gelatinase-associated lipocalin (NGAL) ELISA kit in four populations: HIV-infected patients with TB (HIVpos, TBpos), non-HIV-infected patients with TB (HIVneg, TBpos), HIV-infected patients without TB (HIVpos, TBneg), and healthy controls (HIVneg, TBneg). RESULTS: Serum siderocalin levels were significantly elevated in patients with TB regardless of their HIV status (HIVneg, TBpos 920 (480-1050) pg/ml; HIVpos, TBpos 494 (166-1050) pg/ml), whereas lower levels of siderocalin were seen in HIV-positive patients (HIVpos, TBneg 268 (77-937) pg/ml; HIVneg, TBneg 453 (193-994) pg/ml). CONCLUSIONS: The results indicate that active TB leads to an up-regulation of serum siderocalin regardless of HIV status, whereas HIV infection leads to a down-regulation of serum siderocalin levels in both TB-negative and TB-positive individuals. Further studies are needed to evaluate siderocalin as a potential marker of active TB and to clarify its role in the pathogenesis of HIV-associated TB. SN - 1878-3511 UR - https://www.unboundmedicine.com/medline/citation/31112763/Serum_siderocalin_levels_in_patients_with_tuberculosis_and_HIV_infection L2 - https://linkinghub.elsevier.com/retrieve/pii/S1201-9712(19)30227-9 DB - PRIME DP - Unbound Medicine ER -