Tags

Type your tag names separated by a space and hit enter

Herbs-partitioned moxibustion alleviates aberrant intestinal epithelial cell apoptosis by upregulating A20 expression in a mouse model of Crohn's disease.
World J Gastroenterol. 2019 May 07; 25(17):2071-2085.WJ

Abstract

BACKGROUND

A20 inhibits intestinal epithelial cell apoptosis in Crohn's disease, and herbs-partitioned moxibustion (HPM) has been demonstrated to be an effective treatment for Crohn's disease. However, the mechanism by which HPM reduces intestinal epithelial cell apoptosis in Crohn's disease has not been thoroughly elucidated to date.

AIM

To elucidate whether HPM exerts its effects by upregulating A20 to affect intestinal epithelial cell apoptosis in a Crohn's disease mouse model.

METHODS

In this study, mice with A20 deletion in intestinal epithelial cells (A20IEC-KO) were utilized to establish a Crohn's disease mouse model with 2,4,6-trinitrobenzene sulfonic acid (TNBS) administration, as well as wild-type mice. Mice were randomly divided into normal control (NC), model control (MC), mesalazine (MESA), and HPM groups. The morphology of the colonic mucosa was observed by hematoxylin-eosin staining, and serum endotoxin and apoptosis of epithelial cells were evaluated by enzyme-linked immunosorbent assay and terminal dUTP nick-end labeling assay accordingly. The protein expression levels of A20 and tumor necrosis factor receptor 1 (TNFR1)-related signaling molecules were evaluated by Western blot, and co-expression of A20 and TNFR1-associated death domain (TRADD) and co-expression of A20 and receptor-interacting protein 1 (RIP1) were observed by double immunofluorescence staining.

RESULTS

The intestinal epithelial barrier was noted to have an improvement in the HPM group of wild-type (WT) mice compared with that in A20IEC-KO mice. Compared with A20 IEC-KO HPM mice, serum endotoxin levels and apoptosis percentages were decreased (P < 0.01), A20 expression levels were increased (P < 0.01), and expression of TNFR1, TRADDD, and RIP1 was decreased in the HPM group of WT mice (P TNFR1 < 0.05, P TRADD < 0.01, P RIP1 < 0.01). Both of the co-expression of A20/TRADD and A20/RIP1 showed a predominantly yellow fluorescence in the HPM group of WT mice, while a predominantly red fluorescence was noted in the HPM group of A20IEC-KO mice.

CONCLUSION

Our findings suggest that HPM in treating Crohn's disease functions possibly via upregulation of the A20 expression level, resulting in downregulation of TNFR1, TRADD, and RIP1 to alleviate increased cell apoptosis in the intestinal epithelial barrier in Crohn's disease.

Authors+Show Affiliations

Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.Qigong Institute, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.Key Laboratory of Acupuncture and Immunological Effects, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.Key Laboratory of Acupuncture and Immunological Effects, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.Key Laboratory of Acupuncture and Immunological Effects, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.Key Laboratory of Acupuncture and Immunological Effects, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. flysy0636@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31114134

Citation

Zhou, Jing, et al. "Herbs-partitioned Moxibustion Alleviates Aberrant Intestinal Epithelial Cell Apoptosis By Upregulating A20 Expression in a Mouse Model of Crohn's Disease." World Journal of Gastroenterology, vol. 25, no. 17, 2019, pp. 2071-2085.
Zhou J, Wu LY, Chen L, et al. Herbs-partitioned moxibustion alleviates aberrant intestinal epithelial cell apoptosis by upregulating A20 expression in a mouse model of Crohn's disease. World J Gastroenterol. 2019;25(17):2071-2085.
Zhou, J., Wu, L. Y., Chen, L., Guo, Y. J., Sun, Y., Li, T., Zhao, J. M., Bao, C. H., Wu, H. G., & Shi, Y. (2019). Herbs-partitioned moxibustion alleviates aberrant intestinal epithelial cell apoptosis by upregulating A20 expression in a mouse model of Crohn's disease. World Journal of Gastroenterology, 25(17), 2071-2085. https://doi.org/10.3748/wjg.v25.i17.2071
Zhou J, et al. Herbs-partitioned Moxibustion Alleviates Aberrant Intestinal Epithelial Cell Apoptosis By Upregulating A20 Expression in a Mouse Model of Crohn's Disease. World J Gastroenterol. 2019 May 7;25(17):2071-2085. PubMed PMID: 31114134.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Herbs-partitioned moxibustion alleviates aberrant intestinal epithelial cell apoptosis by upregulating A20 expression in a mouse model of Crohn's disease. AU - Zhou,Jing, AU - Wu,Lu-Yi, AU - Chen,Liu, AU - Guo,Ya-Jing, AU - Sun,Yi, AU - Li,Tao, AU - Zhao,Ji-Meng, AU - Bao,Chun-Hui, AU - Wu,Huan-Gan, AU - Shi,Yin, PY - 2019/01/13/received PY - 2019/03/13/revised PY - 2019/03/15/accepted PY - 2019/5/23/entrez PY - 2019/5/23/pubmed PY - 2019/12/4/medline KW - A20 KW - Apoptotic pathway KW - Crohn’s disease KW - Herbs-partitioned moxibustion KW - Inflammation SP - 2071 EP - 2085 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 25 IS - 17 N2 - BACKGROUND: A20 inhibits intestinal epithelial cell apoptosis in Crohn's disease, and herbs-partitioned moxibustion (HPM) has been demonstrated to be an effective treatment for Crohn's disease. However, the mechanism by which HPM reduces intestinal epithelial cell apoptosis in Crohn's disease has not been thoroughly elucidated to date. AIM: To elucidate whether HPM exerts its effects by upregulating A20 to affect intestinal epithelial cell apoptosis in a Crohn's disease mouse model. METHODS: In this study, mice with A20 deletion in intestinal epithelial cells (A20IEC-KO) were utilized to establish a Crohn's disease mouse model with 2,4,6-trinitrobenzene sulfonic acid (TNBS) administration, as well as wild-type mice. Mice were randomly divided into normal control (NC), model control (MC), mesalazine (MESA), and HPM groups. The morphology of the colonic mucosa was observed by hematoxylin-eosin staining, and serum endotoxin and apoptosis of epithelial cells were evaluated by enzyme-linked immunosorbent assay and terminal dUTP nick-end labeling assay accordingly. The protein expression levels of A20 and tumor necrosis factor receptor 1 (TNFR1)-related signaling molecules were evaluated by Western blot, and co-expression of A20 and TNFR1-associated death domain (TRADD) and co-expression of A20 and receptor-interacting protein 1 (RIP1) were observed by double immunofluorescence staining. RESULTS: The intestinal epithelial barrier was noted to have an improvement in the HPM group of wild-type (WT) mice compared with that in A20IEC-KO mice. Compared with A20 IEC-KO HPM mice, serum endotoxin levels and apoptosis percentages were decreased (P < 0.01), A20 expression levels were increased (P < 0.01), and expression of TNFR1, TRADDD, and RIP1 was decreased in the HPM group of WT mice (P TNFR1 < 0.05, P TRADD < 0.01, P RIP1 < 0.01). Both of the co-expression of A20/TRADD and A20/RIP1 showed a predominantly yellow fluorescence in the HPM group of WT mice, while a predominantly red fluorescence was noted in the HPM group of A20IEC-KO mice. CONCLUSION: Our findings suggest that HPM in treating Crohn's disease functions possibly via upregulation of the A20 expression level, resulting in downregulation of TNFR1, TRADD, and RIP1 to alleviate increased cell apoptosis in the intestinal epithelial barrier in Crohn's disease. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/31114134/Herbs_partitioned_moxibustion_alleviates_aberrant_intestinal_epithelial_cell_apoptosis_by_upregulating_A20_expression_in_a_mouse_model_of_Crohn's_disease_ L2 - http://www.wjgnet.com/1007-9327/full/v25/i17/2071.htm DB - PRIME DP - Unbound Medicine ER -