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YKL-40 promotes the progress of atherosclerosis independent of lipid metabolism in apolipoprotein E-/- mice fed a high-fat diet.

Abstract

YKL-40 is recently regarded as a pro-inflammatory cytokine involved in the pathological process of atherosclerosis and lipid metabolism. However, whether YKL-40 can directly influence the development of atherosclerosis and levels of lipid parameters is unknown. The aim of this study is to explore the effects of YKL-40 on atherosclerotic features, the levels of serum lipids, and biomarkers in apolipoprotein (E)-deficient (ApoE-/-) mice fed a high-fat diet. ApoE-/- mice were injected with a recombinant adenovirus expressing mouse YKL-40 or control adenovirus through the caudal vein. The levels of serum YKL-40, interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-alpha), matrix metalloproteinase-9 (MMP-9), and soluble vascular cell-adhesion molecule 1 (sVCAM-1) were measured by ELISA. Lipid metabolism parameters were measured using immunoturbidimetric assay. The size of plaque area in aorta was evaluated by Oil Red O and hematoxylin/eosin (HE) staining. The content of collagen fibers was stained with Masson, and the content of macrophages and smooth muscle cells (SMCs) in atherosclerotic lesions was investigated by immunohistochemistry. The serum levels of total cholesterol and triglycerides were similar between these two groups. Compared with the control, the levels of serum YKL-40, IL-6, TNF-alpha, MMP-9, plaque size, and macrophages in plaques were significantly increased in mice with adenovirus overexpressing YKL-40. However, the content of collagen fibers and SMCs was remarkably decreased in mice with adenovirus overexpressing YKL-40 than that in control. YKL-40 prompts the progress of atherosclerosis maybe involved with its role of pro-inflammation, but does not affect lipid metabolism in ApoE-/- mice fed a high-fat diet.

Authors+Show Affiliations

Department of Pathology, Zhejiang Provincial People's Hospital, Hangzhou, 310014, Zhejiang, China.Department of Cardiology, Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou, 310014, Zhejiang, China. zhengjianlei@zjheart.com.Department of Cardiology, Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou, 310014, Zhejiang, China.Department of Cardiology, Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou, 310014, Zhejiang, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31114961

Citation

Chen, Lei, et al. "YKL-40 Promotes the Progress of Atherosclerosis Independent of Lipid Metabolism in Apolipoprotein E-/- Mice Fed a High-fat Diet." Heart and Vessels, 2019.
Chen L, Zheng J, Xue Q, et al. YKL-40 promotes the progress of atherosclerosis independent of lipid metabolism in apolipoprotein E-/- mice fed a high-fat diet. Heart Vessels. 2019.
Chen, L., Zheng, J., Xue, Q., & Zhao, Y. (2019). YKL-40 promotes the progress of atherosclerosis independent of lipid metabolism in apolipoprotein E-/- mice fed a high-fat diet. Heart and Vessels, doi:10.1007/s00380-019-01434-w.
Chen L, et al. YKL-40 Promotes the Progress of Atherosclerosis Independent of Lipid Metabolism in Apolipoprotein E-/- Mice Fed a High-fat Diet. Heart Vessels. 2019 May 21; PubMed PMID: 31114961.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - YKL-40 promotes the progress of atherosclerosis independent of lipid metabolism in apolipoprotein E-/- mice fed a high-fat diet. AU - Chen,Lei, AU - Zheng,Jianlei, AU - Xue,Qi, AU - Zhao,Yan, Y1 - 2019/05/21/ PY - 2019/01/15/received PY - 2019/05/15/accepted PY - 2019/5/23/entrez KW - Atherosclerosis KW - Inflammation KW - Lipid metabolism KW - YKL-40 JF - Heart and vessels JO - Heart Vessels N2 - YKL-40 is recently regarded as a pro-inflammatory cytokine involved in the pathological process of atherosclerosis and lipid metabolism. However, whether YKL-40 can directly influence the development of atherosclerosis and levels of lipid parameters is unknown. The aim of this study is to explore the effects of YKL-40 on atherosclerotic features, the levels of serum lipids, and biomarkers in apolipoprotein (E)-deficient (ApoE-/-) mice fed a high-fat diet. ApoE-/- mice were injected with a recombinant adenovirus expressing mouse YKL-40 or control adenovirus through the caudal vein. The levels of serum YKL-40, interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-alpha), matrix metalloproteinase-9 (MMP-9), and soluble vascular cell-adhesion molecule 1 (sVCAM-1) were measured by ELISA. Lipid metabolism parameters were measured using immunoturbidimetric assay. The size of plaque area in aorta was evaluated by Oil Red O and hematoxylin/eosin (HE) staining. The content of collagen fibers was stained with Masson, and the content of macrophages and smooth muscle cells (SMCs) in atherosclerotic lesions was investigated by immunohistochemistry. The serum levels of total cholesterol and triglycerides were similar between these two groups. Compared with the control, the levels of serum YKL-40, IL-6, TNF-alpha, MMP-9, plaque size, and macrophages in plaques were significantly increased in mice with adenovirus overexpressing YKL-40. However, the content of collagen fibers and SMCs was remarkably decreased in mice with adenovirus overexpressing YKL-40 than that in control. YKL-40 prompts the progress of atherosclerosis maybe involved with its role of pro-inflammation, but does not affect lipid metabolism in ApoE-/- mice fed a high-fat diet. SN - 1615-2573 UR - https://www.unboundmedicine.com/medline/citation/31114961/YKL-40_promotes_the_progress_of_atherosclerosis_independent_of_lipid_metabolism_in_apolipoprotein_E-/-_mice_fed_a_high-fat_diet L2 - https://dx.doi.org/10.1007/s00380-019-01434-w DB - PRIME DP - Unbound Medicine ER -