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Role of the Citrus sinensis RNA deadenylase CsCAF1 in citrus canker resistance.
Mol Plant Pathol. 2019 08; 20(8):1105-1118.MP

Abstract

Poly(A) tail shortening is a critical step in messenger RNA (mRNA) decay and control of gene expression. The carbon catabolite repressor 4 (CCR4)-associated factor 1 (CAF1) component of the CCR4-NOT deadenylase complex plays an essential role in mRNA deadenylation in most eukaryotes. However, while CAF1 has been extensively investigated in yeast and animals, its role in plants remains largely unknown. Here, we show that the Citrus sinensis CAF1 (CsCAF1) is a magnesium-dependent deadenylase implicated in resistance against the citrus canker bacteria Xanthomonas citri. CsCAF1 interacted with proteins of the CCR4-NOT complex, including CsVIP2, a NOT2 homologue, translin-associated factor X (CsTRAX) and the poly(A)-binding proteins CsPABPN and CsPABPC. CsCAF1 also interacted with PthA4, the main X. citri effector required for citrus canker elicitation. We also present evidence suggesting that PthA4 inhibits CsCAF1 deadenylase activity in vitro and stabilizes the mRNA encoded by the citrus canker susceptibility gene CsLOB1, which is transcriptionally activated by PthA4 during canker formation. Moreover, we show that an inhibitor of CsCAF1 deadenylase activity significantly enhanced canker development, despite causing a reduction in PthA4-dependent CsLOB1 transcription. These results thus link CsCAF1 with canker development and PthA4-dependent transcription in citrus plants.

Authors+Show Affiliations

Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), CEP 13083-100, Campinas, SP, Brazil.Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), CEP 13083-100, Campinas, SP, Brazil.Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), CEP 13083-100, Campinas, SP, Brazil.Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), CEP 13083-100, Campinas, SP, Brazil.Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), CEP 13083-100, Campinas, SP, Brazil.Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), CEP 13083-100, Campinas, SP, Brazil.Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), CEP 13083-100, Campinas, SP, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31115151

Citation

Shimo, Hugo Massayoshi, et al. "Role of the Citrus Sinensis RNA Deadenylase CsCAF1 in Citrus Canker Resistance." Molecular Plant Pathology, vol. 20, no. 8, 2019, pp. 1105-1118.
Shimo HM, Terassi C, Lima Silva CC, et al. Role of the Citrus sinensis RNA deadenylase CsCAF1 in citrus canker resistance. Mol Plant Pathol. 2019;20(8):1105-1118.
Shimo, H. M., Terassi, C., Lima Silva, C. C., Zanella, J. L., Mercaldi, G. F., Rocco, S. A., & Benedetti, C. E. (2019). Role of the Citrus sinensis RNA deadenylase CsCAF1 in citrus canker resistance. Molecular Plant Pathology, 20(8), 1105-1118. https://doi.org/10.1111/mpp.12815
Shimo HM, et al. Role of the Citrus Sinensis RNA Deadenylase CsCAF1 in Citrus Canker Resistance. Mol Plant Pathol. 2019;20(8):1105-1118. PubMed PMID: 31115151.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of the Citrus sinensis RNA deadenylase CsCAF1 in citrus canker resistance. AU - Shimo,Hugo Massayoshi, AU - Terassi,Carolina, AU - Lima Silva,Caio Cesar, AU - Zanella,Jackeline de Lima, AU - Mercaldi,Gustavo Fernando, AU - Rocco,Silvana Aparecida, AU - Benedetti,Celso Eduardo, Y1 - 2019/05/21/ PY - 2019/5/23/pubmed PY - 2020/6/20/medline PY - 2019/5/23/entrez KW - Citrus sinensis KW - CsLOB1 KW - Xanthomonas aurantifolii KW - Xanthomonas citri KW - CCR4-NOT-associated factor KW - CsCAF1 KW - PthA4 KW - RNA deadenylase activity KW - TAL effectors KW - citrus canker SP - 1105 EP - 1118 JF - Molecular plant pathology JO - Mol. Plant Pathol. VL - 20 IS - 8 N2 - Poly(A) tail shortening is a critical step in messenger RNA (mRNA) decay and control of gene expression. The carbon catabolite repressor 4 (CCR4)-associated factor 1 (CAF1) component of the CCR4-NOT deadenylase complex plays an essential role in mRNA deadenylation in most eukaryotes. However, while CAF1 has been extensively investigated in yeast and animals, its role in plants remains largely unknown. Here, we show that the Citrus sinensis CAF1 (CsCAF1) is a magnesium-dependent deadenylase implicated in resistance against the citrus canker bacteria Xanthomonas citri. CsCAF1 interacted with proteins of the CCR4-NOT complex, including CsVIP2, a NOT2 homologue, translin-associated factor X (CsTRAX) and the poly(A)-binding proteins CsPABPN and CsPABPC. CsCAF1 also interacted with PthA4, the main X. citri effector required for citrus canker elicitation. We also present evidence suggesting that PthA4 inhibits CsCAF1 deadenylase activity in vitro and stabilizes the mRNA encoded by the citrus canker susceptibility gene CsLOB1, which is transcriptionally activated by PthA4 during canker formation. Moreover, we show that an inhibitor of CsCAF1 deadenylase activity significantly enhanced canker development, despite causing a reduction in PthA4-dependent CsLOB1 transcription. These results thus link CsCAF1 with canker development and PthA4-dependent transcription in citrus plants. SN - 1364-3703 UR - https://www.unboundmedicine.com/medline/citation/31115151/Role_of_the_Citrus_sinensis_RNA_deadenylase_CsCAF1_in_citrus_canker_resistance_ L2 - https://doi.org/10.1111/mpp.12815 DB - PRIME DP - Unbound Medicine ER -