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Expression of integrins to control migration direction of electrotaxis.
FASEB J 2019; 33(8):9131-9141FJ

Abstract

Proper control of cell migration is critically important in many biologic processes, such as wound healing, immune surveillance, and development. Much progress has been made in the initiation of cell migration; however, little is known about termination and sometimes directional reversal. During active cell migration, as in wound healing, development, and immune surveillance, the integrin expression profile undergoes drastic changes. Here, we uncovered the extensive regulatory and even opposing roles of integrins in directional cell migration in electric fields (EFs), a potentially important endogenous guidance mechanism. We established cell lines that stably express specific integrins and determined their responses to applied EFs with a high throughput screen. Expression of specific integrins drove cells to migrate to the cathode or to the anode or to lose migration direction. Cells expressing αMβ2, β1, α2, αIIbβ3, and α5 migrated to the cathode, whereas cells expressing β3, α6, and α9 migrated to the anode. Cells expressing α4, αV, and α6β4 lost directional electrotaxis. Manipulation of α9 molecules, one of the molecular directional switches, suggested that the intracellular domain is critical for the directional reversal. These data revealed an unreported role for integrins in controlling stop, go, and reversal activity of directional migration of mammalian cells in EFs, which might ensure that cells reach their final destination with well-controlled speed and direction.-Zhu, K., Takada, Y., Nakajima, K., Sun, Y., Jiang, J., Zhang, Y., Zeng, Q., Takada, Y., Zhao, M. Expression of integrins to control migration direction of electrotaxis.

Authors+Show Affiliations

Department of Dermatology, School of Medicine, University of California-Davis, Sacramento, California, USA. Institute for Regenerative Cures, University of California-Davis, Sacramento, California, USA. State Key Laboratory of Trauma, Burns, and Combined Injury, Institute of Surgery Research, Third Military Medical University, Chongqing, China.Department of Dermatology, School of Medicine, University of California-Davis, Sacramento, California, USA.Department of Dermatology, School of Medicine, University of California-Davis, Sacramento, California, USA. Institute for Regenerative Cures, University of California-Davis, Sacramento, California, USA.Department of Dermatology, School of Medicine, University of California-Davis, Sacramento, California, USA. Institute for Regenerative Cures, University of California-Davis, Sacramento, California, USA.State Key Laboratory of Trauma, Burns, and Combined Injury, Institute of Surgery Research, Third Military Medical University, Chongqing, China.Department of Dermatology, School of Medicine, University of California-Davis, Sacramento, California, USA. Institute for Regenerative Cures, University of California-Davis, Sacramento, California, USA. Bioelectromagnetics Laboratory, Zhejiang University School of Medicine, Hangzhou, China.Bioelectromagnetics Laboratory, Zhejiang University School of Medicine, Hangzhou, China.Department of Dermatology, School of Medicine, University of California-Davis, Sacramento, California, USA.Department of Dermatology, School of Medicine, University of California-Davis, Sacramento, California, USA. Institute for Regenerative Cures, University of California-Davis, Sacramento, California, USA. Department of Ophthalmology and Vision Science, School of Medicine, University of California-Davis, Sacramento, California, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31116572

Citation

Zhu, Kan, et al. "Expression of Integrins to Control Migration Direction of Electrotaxis." FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, vol. 33, no. 8, 2019, pp. 9131-9141.
Zhu K, Takada Y, Nakajima K, et al. Expression of integrins to control migration direction of electrotaxis. FASEB J. 2019;33(8):9131-9141.
Zhu, K., Takada, Y., Nakajima, K., Sun, Y., Jiang, J., Zhang, Y., ... Zhao, M. (2019). Expression of integrins to control migration direction of electrotaxis. FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 33(8), pp. 9131-9141. doi:10.1096/fj.201802657R.
Zhu K, et al. Expression of Integrins to Control Migration Direction of Electrotaxis. FASEB J. 2019;33(8):9131-9141. PubMed PMID: 31116572.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of integrins to control migration direction of electrotaxis. AU - Zhu,Kan, AU - Takada,Yoko, AU - Nakajima,Kenichi, AU - Sun,Yaohui, AU - Jiang,Jianxin, AU - Zhang,Yan, AU - Zeng,Qunli, AU - Takada,Yoshikazu, AU - Zhao,Min, Y1 - 2019/05/22/ PY - 2020/05/22/pmc-release PY - 2019/5/23/pubmed PY - 2019/5/23/medline PY - 2019/5/23/entrez KW - directional cell migration KW - directional reversal KW - galvanotaxis KW - motility KW - α9 SP - 9131 EP - 9141 JF - FASEB journal : official publication of the Federation of American Societies for Experimental Biology JO - FASEB J. VL - 33 IS - 8 N2 - Proper control of cell migration is critically important in many biologic processes, such as wound healing, immune surveillance, and development. Much progress has been made in the initiation of cell migration; however, little is known about termination and sometimes directional reversal. During active cell migration, as in wound healing, development, and immune surveillance, the integrin expression profile undergoes drastic changes. Here, we uncovered the extensive regulatory and even opposing roles of integrins in directional cell migration in electric fields (EFs), a potentially important endogenous guidance mechanism. We established cell lines that stably express specific integrins and determined their responses to applied EFs with a high throughput screen. Expression of specific integrins drove cells to migrate to the cathode or to the anode or to lose migration direction. Cells expressing αMβ2, β1, α2, αIIbβ3, and α5 migrated to the cathode, whereas cells expressing β3, α6, and α9 migrated to the anode. Cells expressing α4, αV, and α6β4 lost directional electrotaxis. Manipulation of α9 molecules, one of the molecular directional switches, suggested that the intracellular domain is critical for the directional reversal. These data revealed an unreported role for integrins in controlling stop, go, and reversal activity of directional migration of mammalian cells in EFs, which might ensure that cells reach their final destination with well-controlled speed and direction.-Zhu, K., Takada, Y., Nakajima, K., Sun, Y., Jiang, J., Zhang, Y., Zeng, Q., Takada, Y., Zhao, M. Expression of integrins to control migration direction of electrotaxis. SN - 1530-6860 UR - https://www.unboundmedicine.com/medline/citation/31116572/Expression_of_integrins_to_control_migration_direction_of_electrotaxis L2 - http://www.fasebj.org/doi/full/10.1096/fj.201802657R?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -