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Role of MHC-I Expression on Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in Mice.
Cells. 2019 05 21; 8(5)C

Abstract

Lesions to the CNS/PNS interface are especially severe, leading to elevated neuronal degeneration. In the present work, we establish the ventral root crush model for mice, and demonstrate the potential of such an approach, by analyzing injury evoked motoneuron loss, changes of synaptic coverage and concomitant glial responses in β2-microglobulin knockout mice (β2m KO). Young adult (8-12 weeks old) C57BL/6J (WT) and β2m KO mice were submitted to a L4-L6 ventral roots crush. Neuronal survival revealed a time-dependent motoneuron-like cell loss, both in WT and β2m KO mice. Along with neuronal loss, astrogliosis increased in WT mice, which was not observed in β2m KO mice. Microglial responses were more pronounced during the acute phase after lesion and decreased over time, in WT and KO mice. At 7 days after lesion β2m KO mice showed stronger Iba-1+ cell reaction. The synaptic inputs were reduced over time, but in β2m KO, the synaptic loss was more prominent between 7 and 28 days after lesion. Taken together, the results herein demonstrate that ventral root crushing in mice provides robust data regarding neuronal loss and glial reaction. The retrograde reactions after injury were altered in the absence of functional MHC-I surface expression.

Authors+Show Affiliations

Laboratory of Nerve Regeneration, University of Campinas-UNICAMP, Cidade Universitaria "Zeferino Vaz, Rua Monteiro Lobato, 255, 13083-970 Campinas, SP, Brazil. lucartarozzi@gmail.com.School of Physical Education and Sport of Ribeirao Preto, University of Sao Paulo, Av. Bandeirantes, 3900, 14040-907 Ribeirão Preto, SP, Brazil. matheusperez@usp.br.Molecular Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), University of Saarland, Building 48, 66421 Homburg, Germany. Frank.Kirchhoff@uks.eu.Laboratory of Nerve Regeneration, University of Campinas-UNICAMP, Cidade Universitaria "Zeferino Vaz, Rua Monteiro Lobato, 255, 13083-970 Campinas, SP, Brazil. alroliv@unicamp.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31117227

Citation

Cartarozzi, Luciana Politti, et al. "Role of MHC-I Expression On Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in Mice." Cells, vol. 8, no. 5, 2019.
Cartarozzi LP, Perez M, Kirchhoff F, et al. Role of MHC-I Expression on Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in Mice. Cells. 2019;8(5).
Cartarozzi, L. P., Perez, M., Kirchhoff, F., & Oliveira, A. L. R. (2019). Role of MHC-I Expression on Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in Mice. Cells, 8(5). https://doi.org/10.3390/cells8050483
Cartarozzi LP, et al. Role of MHC-I Expression On Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in Mice. Cells. 2019 05 21;8(5) PubMed PMID: 31117227.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of MHC-I Expression on Spinal Motoneuron Survival and Glial Reactions Following Ventral Root Crush in Mice. AU - Cartarozzi,Luciana Politti, AU - Perez,Matheus, AU - Kirchhoff,Frank, AU - Oliveira,Alexandre Leite Rodrigues de, Y1 - 2019/05/21/ PY - 2019/02/26/received PY - 2019/05/05/revised PY - 2019/05/07/accepted PY - 2019/5/24/entrez PY - 2019/5/24/pubmed PY - 2019/5/24/medline KW - PNS/CNS interface KW - astrogliosis KW - microglial reaction KW - synaptic covering KW - β2m knockout mice JF - Cells JO - Cells VL - 8 IS - 5 N2 - Lesions to the CNS/PNS interface are especially severe, leading to elevated neuronal degeneration. In the present work, we establish the ventral root crush model for mice, and demonstrate the potential of such an approach, by analyzing injury evoked motoneuron loss, changes of synaptic coverage and concomitant glial responses in β2-microglobulin knockout mice (β2m KO). Young adult (8-12 weeks old) C57BL/6J (WT) and β2m KO mice were submitted to a L4-L6 ventral roots crush. Neuronal survival revealed a time-dependent motoneuron-like cell loss, both in WT and β2m KO mice. Along with neuronal loss, astrogliosis increased in WT mice, which was not observed in β2m KO mice. Microglial responses were more pronounced during the acute phase after lesion and decreased over time, in WT and KO mice. At 7 days after lesion β2m KO mice showed stronger Iba-1+ cell reaction. The synaptic inputs were reduced over time, but in β2m KO, the synaptic loss was more prominent between 7 and 28 days after lesion. Taken together, the results herein demonstrate that ventral root crushing in mice provides robust data regarding neuronal loss and glial reaction. The retrograde reactions after injury were altered in the absence of functional MHC-I surface expression. SN - 2073-4409 UR - https://www.unboundmedicine.com/medline/citation/31117227/Role_of_MHC_I_Expression_on_Spinal_Motoneuron_Survival_and_Glial_Reactions_Following_Ventral_Root_Crush_in_Mice_ L2 - http://www.mdpi.com/resolver?pii=cells8050483 DB - PRIME DP - Unbound Medicine ER -