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Palindromic Fragment-Mediated Single-Chain Amplification: An Innovative Mode for Photoelectrochemical Bioassay.
Anal Chem. 2019 06 18; 91(12):7835-7841.AC

Abstract

This work reports a strategy for glutathione-loaded liposome-encoded magnetic beads initiated by palindromic fragment-mediated single-chain amplification (PFMSCA) for high-precision quantification of a low-abundance aminoglycoside antibiotic (kanamycin; Kana) by using In2O3-ZnIn2S4 (IO-ZIS) as a photoactive matrix. In this strategy, a Kana-recognition region, primer-like palindromic fragment, and polymerization/nicking template are reasonably integrated into one oligonucleotide (hairpin HP1) for target recognition, magnetic separation, and target amplification. Upon target Kana introduction, the Kana-aptamer region in HP1 specifically recognizes the Kana and triggers the palindromic tails intramolecular self-hybridization, amplifying a large number of short fragments in the presence of Klenow fragment polymerase and Nt.BbvCI. The as-generated nick fragments act as a linker to introduce the free hairpin HP2-functionalized glutathione-loaded liposomes (HP2-GLL) onto the surface of the hairpin HP3-modified magnetic beads (HP3-MB), constructing liposome-encoded magnetic beads (HP3-MB-nick-HP2-GLL). Following magnetic separation, the detached glutathione-loaded liposomes (GLL) are lysed by treatment with 1% Triton X-100 to release the glutathione within it, which were then detected as an amplified photocurrent at the IO-ZIS-based photoelectrode. Importantly, this method can be readily carried out by using one oligonucleotide to achieve an exponential amplification effect and open new opportunities for advanced development of robust biodetection systems.

Authors+Show Affiliations

Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry , Fuzhou University , Fuzhou 350108 , People's Republic of China.Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry , Fuzhou University , Fuzhou 350108 , People's Republic of China.Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry , Fuzhou University , Fuzhou 350108 , People's Republic of China.Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry , Fuzhou University , Fuzhou 350108 , People's Republic of China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31117410

Citation

Zeng, Ruijin, et al. "Palindromic Fragment-Mediated Single-Chain Amplification: an Innovative Mode for Photoelectrochemical Bioassay." Analytical Chemistry, vol. 91, no. 12, 2019, pp. 7835-7841.
Zeng R, Zhang L, Luo Z, et al. Palindromic Fragment-Mediated Single-Chain Amplification: An Innovative Mode for Photoelectrochemical Bioassay. Anal Chem. 2019;91(12):7835-7841.
Zeng, R., Zhang, L., Luo, Z., & Tang, D. (2019). Palindromic Fragment-Mediated Single-Chain Amplification: An Innovative Mode for Photoelectrochemical Bioassay. Analytical Chemistry, 91(12), 7835-7841. https://doi.org/10.1021/acs.analchem.9b01557
Zeng R, et al. Palindromic Fragment-Mediated Single-Chain Amplification: an Innovative Mode for Photoelectrochemical Bioassay. Anal Chem. 2019 06 18;91(12):7835-7841. PubMed PMID: 31117410.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Palindromic Fragment-Mediated Single-Chain Amplification: An Innovative Mode for Photoelectrochemical Bioassay. AU - Zeng,Ruijin, AU - Zhang,Lijia, AU - Luo,Zhongbin, AU - Tang,Dianping, Y1 - 2019/05/24/ PY - 2019/5/24/pubmed PY - 2020/9/18/medline PY - 2019/5/24/entrez SP - 7835 EP - 7841 JF - Analytical chemistry JO - Anal Chem VL - 91 IS - 12 N2 - This work reports a strategy for glutathione-loaded liposome-encoded magnetic beads initiated by palindromic fragment-mediated single-chain amplification (PFMSCA) for high-precision quantification of a low-abundance aminoglycoside antibiotic (kanamycin; Kana) by using In2O3-ZnIn2S4 (IO-ZIS) as a photoactive matrix. In this strategy, a Kana-recognition region, primer-like palindromic fragment, and polymerization/nicking template are reasonably integrated into one oligonucleotide (hairpin HP1) for target recognition, magnetic separation, and target amplification. Upon target Kana introduction, the Kana-aptamer region in HP1 specifically recognizes the Kana and triggers the palindromic tails intramolecular self-hybridization, amplifying a large number of short fragments in the presence of Klenow fragment polymerase and Nt.BbvCI. The as-generated nick fragments act as a linker to introduce the free hairpin HP2-functionalized glutathione-loaded liposomes (HP2-GLL) onto the surface of the hairpin HP3-modified magnetic beads (HP3-MB), constructing liposome-encoded magnetic beads (HP3-MB-nick-HP2-GLL). Following magnetic separation, the detached glutathione-loaded liposomes (GLL) are lysed by treatment with 1% Triton X-100 to release the glutathione within it, which were then detected as an amplified photocurrent at the IO-ZIS-based photoelectrode. Importantly, this method can be readily carried out by using one oligonucleotide to achieve an exponential amplification effect and open new opportunities for advanced development of robust biodetection systems. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/31117410/Palindromic_Fragment_Mediated_Single_Chain_Amplification:_An_Innovative_Mode_for_Photoelectrochemical_Bioassay_ DB - PRIME DP - Unbound Medicine ER -