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Effect of Thymol and Carvacrol Encapsulated in Hp-Β-Cyclodextrin by Two Inclusion Methods against Geotrichum citri-aurantii.
J Food Sci. 2019 Jun; 84(6):1513-1521.JF

Abstract

Geotrichum citri-aurantii causes sour rot in citrus fruits and is responsible for important economic losses during storage. However, the availability of chemical fungicides for the control of this pathogen is limited. Thus, the aim of this research was to evaluate the antifungal efficacy of thymol and carvacrol encapsulated in 2-hydroxylpropyl-beta-cyclodextrin (HP-β-CD) (prepared by the microwave irradiation method [MW] and solubility method [S]) for inhibition of G. citri-aurantii using in vitro bioassays broth (micro and macrodilutions methods) and inoculated food testing. Both encapsulated thymol and carvacrol were shown to be effective for inhibiting G. citri-aurantii growth in in vitro assays. Thymol was more effective in inhibiting G. citri-aurantii, while better encapsulation was provided by MW. HP-β-CD-thymol encapsulated by MW (HP-β-CD-thymol-MW) showed the lowest 50% effective dose (ED50 = 1.16 mM), minimum inhibitory concentration (MIC = 5.06 mM), and minimum fungicide concentration (MFC = 52.6 mM). HP-β-CD-thymol-MW was found highly effective in reducing the growth rate and mycelial growth inhibition. Finally, HP-β-CD-thymol-MW and HP-β-CD-carvacrol-MW showed a higher persistent effect than thymol and carvacrol in their natural form in inhibiting this fungus. Therefore, HP-β-CD-thymol-MW could be a promising alternative to synthetic fungicides for controlling G. citri-aurantii, the causal agent of citrus sour rot. PRACTICAL APPLICATION: Encapsulated thymol and carvacrol in HP-β-Cyclodextrins are effective for controlling G. citri-aurantii in in vitro experiments. Encapsulation of thymol and carvacrol by microwave irradiation method (MW) was more effective than the solubility (S) method. Thymol was more effective than carvacrol, and the best results on G. citri-auriantii inhibition were achieved using the HP-β-CD-thymol-MW method (which gave the lowest ED50 , MIC, and MFC).

Authors+Show Affiliations

Dept. of Food Technology, Univ. Miguel Hernández, Ctra. Beniel km. 3.2, 03312, Orihuela, Alicante, Spain.Dept. of Applied Biology, Univ. Miguel Hernández, Ctra. Beniel km. 3.2, 03312, Orihuela, Alicante, Spain.Dept. of Food Technology, Univ. Miguel Hernández, Ctra. Beniel km. 3.2, 03312, Orihuela, Alicante, Spain.Dept. of Food Technology and Nutrition, Univ. Católica San Antonio de Murcia (UCAM), Avenida de los Jerónimos s/n, 30107, Guadalupe, Murcia, Spain.Dept. of Food Technology and Nutrition, Univ. Católica San Antonio de Murcia (UCAM), Avenida de los Jerónimos s/n, 30107, Guadalupe, Murcia, Spain.Dept. of Food Technology, Univ. Miguel Hernández, Ctra. Beniel km. 3.2, 03312, Orihuela, Alicante, Spain.Dept. of Food Technology, Univ. Miguel Hernández, Ctra. Beniel km. 3.2, 03312, Orihuela, Alicante, Spain.Dept. of Food Technology, Univ. Miguel Hernández, Ctra. Beniel km. 3.2, 03312, Orihuela, Alicante, Spain.Dept. of Food Technology, Univ. Miguel Hernández, Ctra. Beniel km. 3.2, 03312, Orihuela, Alicante, Spain.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

31120593

Citation

Serna-Escolano, Vicente, et al. "Effect of Thymol and Carvacrol Encapsulated in Hp-Β-Cyclodextrin By Two Inclusion Methods Against Geotrichum Citri-aurantii." Journal of Food Science, vol. 84, no. 6, 2019, pp. 1513-1521.
Serna-Escolano V, Serrano M, Valero D, et al. Effect of Thymol and Carvacrol Encapsulated in Hp-Β-Cyclodextrin by Two Inclusion Methods against Geotrichum citri-aurantii. J Food Sci. 2019;84(6):1513-1521.
Serna-Escolano, V., Serrano, M., Valero, D., Rodríguez-López, M. I., Gabaldón, J. A., Castillo, S., Guillén, F., Zapata, P. J., & Martínez-Romero, D. (2019). Effect of Thymol and Carvacrol Encapsulated in Hp-Β-Cyclodextrin by Two Inclusion Methods against Geotrichum citri-aurantii. Journal of Food Science, 84(6), 1513-1521. https://doi.org/10.1111/1750-3841.14670
Serna-Escolano V, et al. Effect of Thymol and Carvacrol Encapsulated in Hp-Β-Cyclodextrin By Two Inclusion Methods Against Geotrichum Citri-aurantii. J Food Sci. 2019;84(6):1513-1521. PubMed PMID: 31120593.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of Thymol and Carvacrol Encapsulated in Hp-Β-Cyclodextrin by Two Inclusion Methods against Geotrichum citri-aurantii. AU - Serna-Escolano,Vicente, AU - Serrano,María, AU - Valero,Daniel, AU - Rodríguez-López,María Isabel, AU - Gabaldón,José Antonio, AU - Castillo,Salvador, AU - Guillén,Fabián, AU - Zapata,Pedro Javier, AU - Martínez-Romero,Domingo, Y1 - 2019/05/23/ PY - 2019/01/27/received PY - 2019/04/30/revised PY - 2019/05/02/accepted PY - 2019/5/24/pubmed PY - 2019/8/20/medline PY - 2019/5/24/entrez KW - citrus KW - cyclodextrin KW - decay KW - essential oils KW - postharvest KW - sour rot SP - 1513 EP - 1521 JF - Journal of food science JO - J. Food Sci. VL - 84 IS - 6 N2 - Geotrichum citri-aurantii causes sour rot in citrus fruits and is responsible for important economic losses during storage. However, the availability of chemical fungicides for the control of this pathogen is limited. Thus, the aim of this research was to evaluate the antifungal efficacy of thymol and carvacrol encapsulated in 2-hydroxylpropyl-beta-cyclodextrin (HP-β-CD) (prepared by the microwave irradiation method [MW] and solubility method [S]) for inhibition of G. citri-aurantii using in vitro bioassays broth (micro and macrodilutions methods) and inoculated food testing. Both encapsulated thymol and carvacrol were shown to be effective for inhibiting G. citri-aurantii growth in in vitro assays. Thymol was more effective in inhibiting G. citri-aurantii, while better encapsulation was provided by MW. HP-β-CD-thymol encapsulated by MW (HP-β-CD-thymol-MW) showed the lowest 50% effective dose (ED50 = 1.16 mM), minimum inhibitory concentration (MIC = 5.06 mM), and minimum fungicide concentration (MFC = 52.6 mM). HP-β-CD-thymol-MW was found highly effective in reducing the growth rate and mycelial growth inhibition. Finally, HP-β-CD-thymol-MW and HP-β-CD-carvacrol-MW showed a higher persistent effect than thymol and carvacrol in their natural form in inhibiting this fungus. Therefore, HP-β-CD-thymol-MW could be a promising alternative to synthetic fungicides for controlling G. citri-aurantii, the causal agent of citrus sour rot. PRACTICAL APPLICATION: Encapsulated thymol and carvacrol in HP-β-Cyclodextrins are effective for controlling G. citri-aurantii in in vitro experiments. Encapsulation of thymol and carvacrol by microwave irradiation method (MW) was more effective than the solubility (S) method. Thymol was more effective than carvacrol, and the best results on G. citri-auriantii inhibition were achieved using the HP-β-CD-thymol-MW method (which gave the lowest ED50 , MIC, and MFC). SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/31120593/Effect_of_Thymol_and_Carvacrol_Encapsulated_in_Hp_Β_Cyclodextrin_by_Two_Inclusion_Methods_against_Geotrichum_citri_aurantii_ L2 - https://doi.org/10.1111/1750-3841.14670 DB - PRIME DP - Unbound Medicine ER -