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Isolating Rat Intestinal Explants for In Vitro Cultures.

Abstract

The small intestine is an important organ primarily involved in digestion of food and absorption of nutrients. In vitro intestinal models are being developed to study this organ in health and disease. Intestinal explants can be used in such investigations since they contain all the major intestinal cell types. A detailed procedure to isolate intestinal explants from the rat jejunum is described. A protocol for culturing them in vitro for up to 24 hr is also provided. © 2019 by John Wiley & Sons, Inc.

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  • Authors+Show Affiliations

    ,

    School of Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, Virginia.

    School of Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, Virginia. Department of Chemical Engineering, Virginia Tech, Blacksburg, Virginia. ICTAS Center for Systems Biology of Engineered Tissues, Virginia Tech, Blacksburg, Virginia.

    Source

    Current protocols in toxicology 80:1 2019 Jun pg e79

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    31120607

    Citation

    Kothari, Anjaney, and Padmavathy Rajagopalan. "Isolating Rat Intestinal Explants for in Vitro Cultures." Current Protocols in Toxicology, vol. 80, no. 1, 2019, pp. e79.
    Kothari A, Rajagopalan P. Isolating Rat Intestinal Explants for In Vitro Cultures. Curr Protoc Toxicol. 2019;80(1):e79.
    Kothari, A., & Rajagopalan, P. (2019). Isolating Rat Intestinal Explants for In Vitro Cultures. Current Protocols in Toxicology, 80(1), pp. e79. doi:10.1002/cptx.79.
    Kothari A, Rajagopalan P. Isolating Rat Intestinal Explants for in Vitro Cultures. Curr Protoc Toxicol. 2019;80(1):e79. PubMed PMID: 31120607.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Isolating Rat Intestinal Explants for In Vitro Cultures. AU - Kothari,Anjaney, AU - Rajagopalan,Padmavathy, Y1 - 2019/05/23/ PY - 2019/5/24/pubmed PY - 2019/5/24/medline PY - 2019/5/24/entrez KW - explant culture KW - in vitro KW - inverted KW - small intestine SP - e79 EP - e79 JF - Current protocols in toxicology JO - Curr Protoc Toxicol VL - 80 IS - 1 N2 - The small intestine is an important organ primarily involved in digestion of food and absorption of nutrients. In vitro intestinal models are being developed to study this organ in health and disease. Intestinal explants can be used in such investigations since they contain all the major intestinal cell types. A detailed procedure to isolate intestinal explants from the rat jejunum is described. A protocol for culturing them in vitro for up to 24 hr is also provided. © 2019 by John Wiley & Sons, Inc. SN - 1934-9262 UR - https://www.unboundmedicine.com/medline/citation/31120607/Isolating_Rat_Intestinal_Explants_for_In_Vitro_Cultures L2 - https://dx.doi.org/10.1002/cptx.79 DB - PRIME DP - Unbound Medicine ER -