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A portable and universal upconversion nanoparticle-based lateral flow assay platform for point-of-care testing.
Talanta. 2019 Aug 15; 201:126-133.T

Abstract

Upconversion nanoparticle-based lateral flow assays (UCNP-LFAs) have attracted significant attention in point-of-care testing (POCT) applications, due to the long-term photostability and enhanced signal-to-background noise ratio. The existing UCNP-LFAs generally require peripheral equipment for exciting fluorescent signals and reading out fluorescence results, which are generally bulky and expensive. Herein, we developed a miniaturized and portable UCNP-LFA platform, which is composed of a LFA detection system, an UCNP-LFA reader and a smartphone-assisted UCNP-LFA analyzer. The LFA detection system is based on three types of UCNPs for multiplexed detection. The reader has a dimension of 24.0 cm × 9.4 cm × 5.4 cm (L × W × H) and weight of 0.9 kg. The analyzer based on the custom-designed software of a smartphone (termed as UCNP-LFA analyzer) can get the quantitative analysis results in a real-time manner. We demonstrated the universality of this platform by highly sensitive and quantitative detections of several kinds of targets, including small molecule (ochratoxin A, OTA), heavy metal ion (Hg2+), bacteria (salmonella, SE), nucleic acid (hepatitis B virus, HBV) and protein (growth stimulation expressed gene 2, ST-2). Our developed UCNP-LFA platform holds great promise for applications in disease diagnostics, environmental pollution monitoring and food safety at the point of care.

Authors+Show Affiliations

The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an, PR China.The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an, PR China.The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an, PR China.The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an, PR China.The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an, PR China.Department of Chemistry and Biochemistry, Biomedical Engineering, Border Biomedical Research Center, Environmental Science and Engineering, University of Texas at El Paso, 500 West University Avenue, El Paso, TX, 79968, United States.The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an, PR China.The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an, PR China.The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an, PR China. Electronic address: feili@mail.xjtu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31122402

Citation

Gong, Yan, et al. "A Portable and Universal Upconversion Nanoparticle-based Lateral Flow Assay Platform for Point-of-care Testing." Talanta, vol. 201, 2019, pp. 126-133.
Gong Y, Zheng Y, Jin B, et al. A portable and universal upconversion nanoparticle-based lateral flow assay platform for point-of-care testing. Talanta. 2019;201:126-133.
Gong, Y., Zheng, Y., Jin, B., You, M., Wang, J., Li, X., Lin, M., Xu, F., & Li, F. (2019). A portable and universal upconversion nanoparticle-based lateral flow assay platform for point-of-care testing. Talanta, 201, 126-133. https://doi.org/10.1016/j.talanta.2019.03.105
Gong Y, et al. A Portable and Universal Upconversion Nanoparticle-based Lateral Flow Assay Platform for Point-of-care Testing. Talanta. 2019 Aug 15;201:126-133. PubMed PMID: 31122402.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A portable and universal upconversion nanoparticle-based lateral flow assay platform for point-of-care testing. AU - Gong,Yan, AU - Zheng,Yamin, AU - Jin,Birui, AU - You,Minli, AU - Wang,Jiayu, AU - Li,XiuJun, AU - Lin,Min, AU - Xu,Feng, AU - Li,Fei, Y1 - 2019/04/03/ PY - 2019/01/09/received PY - 2019/03/24/revised PY - 2019/03/30/accepted PY - 2019/5/25/entrez PY - 2019/5/28/pubmed PY - 2019/6/18/medline KW - Miniaturized device KW - Multiplexed detection KW - Paper microfluidics KW - Point-of-care diagnostics KW - Telemedicine KW - UCNP-based biosensor SP - 126 EP - 133 JF - Talanta JO - Talanta VL - 201 N2 - Upconversion nanoparticle-based lateral flow assays (UCNP-LFAs) have attracted significant attention in point-of-care testing (POCT) applications, due to the long-term photostability and enhanced signal-to-background noise ratio. The existing UCNP-LFAs generally require peripheral equipment for exciting fluorescent signals and reading out fluorescence results, which are generally bulky and expensive. Herein, we developed a miniaturized and portable UCNP-LFA platform, which is composed of a LFA detection system, an UCNP-LFA reader and a smartphone-assisted UCNP-LFA analyzer. The LFA detection system is based on three types of UCNPs for multiplexed detection. The reader has a dimension of 24.0 cm × 9.4 cm × 5.4 cm (L × W × H) and weight of 0.9 kg. The analyzer based on the custom-designed software of a smartphone (termed as UCNP-LFA analyzer) can get the quantitative analysis results in a real-time manner. We demonstrated the universality of this platform by highly sensitive and quantitative detections of several kinds of targets, including small molecule (ochratoxin A, OTA), heavy metal ion (Hg2+), bacteria (salmonella, SE), nucleic acid (hepatitis B virus, HBV) and protein (growth stimulation expressed gene 2, ST-2). Our developed UCNP-LFA platform holds great promise for applications in disease diagnostics, environmental pollution monitoring and food safety at the point of care. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/31122402/A_portable_and_universal_upconversion_nanoparticle_based_lateral_flow_assay_platform_for_point_of_care_testing_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(19)30373-X DB - PRIME DP - Unbound Medicine ER -