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The coupling of immunomagnetic enrichment of bacteria with paper-based platform.
Talanta. 2019 Aug 15; 201:245-252.T

Abstract

In this study, the coupling of magnetic enrichment of bacteria from real samples with rapid surface enhanced Raman spectroscopy (SERS) detection was reported. The selective isolation and enrichment for the model bacteria Escherichia coli (E. coli) was performed using E. coli (primary) antibody bound-magnetic gold (Fe3O4@Au) nanoparticles. Following isolation and enrichment, the rennet enzyme was used to cleave of casein modified Fe3O4/Au-PEI nanoparticles from primary antibody-bound bacteria to prevent the nanoparticle aggregation and provide the movement of bacteria on nitrocellulose membrane. In the first part of the study, optimization studies were carried out namely; the amounts of gold nanoparticles (AuNPs), polyethyleneimine coated magnetic gold (Fe3O4/Au-PEI) nanoparticles, casein and rennet enzyme. The SERS signals of DTNB (5,5'-Dithiobis(2-nitrobenzoic acid)) molecule were collected on the test line and a calibration curve was plotted by using signal intensities. The correlation between the concentration of E. coli and SERS signal was found to be linear within the range of 10[1]-10[7] cfu/mL (R[2] = 0.984, LOD = 0.52 cfu/mL and LOQ = 1.57 cfu/mL). The selectivity of the paper-based lateral flow immunoassay (LFIA) was examined with Bacillus subtilis (B. subtilis), Micrococcus luteus (M. luteus), Salmonella enteritidis (S. enteritidis) which did not produce any significant response compared with E. coli measurement. Finally, the developed paper-based LFIA was tested with urine and milk samples. The obtained SERS results were compared with a plate counting method results which were in a good accordance. The developed method was found as rapid and sensitive to E. coli with a total analysis time of less than 60 min.

Authors+Show Affiliations

Department of Nanotechnology, Faculty of Science, Hacettepe University, 06800, Ankara, Turkey.Department of Food Engineering, Faculty of Engineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey.Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, Etiler, 06330, Ankara, Turkey.Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, Etiler, 06330, Ankara, Turkey.Department of Food Engineering, Faculty of Engineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey.Science Teaching Programme, Faculty of Education, Gazi University, Besevler, 06500, Ankara, Turkey.Department of Biology, Faculty of Science, Gazi University, Besevler, 06500, Ankara, Turkey.Department of Nanotechnology, Faculty of Science, Hacettepe University, 06800, Ankara, Turkey.Department of Food Engineering, Faculty of Engineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey.Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, Etiler, 06330, Ankara, Turkey. Electronic address: utamer@gazi.edu.tr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31122419

Citation

Ilhan, Hasan, et al. "The Coupling of Immunomagnetic Enrichment of Bacteria With Paper-based Platform." Talanta, vol. 201, 2019, pp. 245-252.
Ilhan H, Guven B, Dogan U, et al. The coupling of immunomagnetic enrichment of bacteria with paper-based platform. Talanta. 2019;201:245-252.
Ilhan, H., Guven, B., Dogan, U., Torul, H., Evran, S., Çetin, D., Suludere, Z., Saglam, N., Boyaci, İ. H., & Tamer, U. (2019). The coupling of immunomagnetic enrichment of bacteria with paper-based platform. Talanta, 201, 245-252. https://doi.org/10.1016/j.talanta.2019.04.017
Ilhan H, et al. The Coupling of Immunomagnetic Enrichment of Bacteria With Paper-based Platform. Talanta. 2019 Aug 15;201:245-252. PubMed PMID: 31122419.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The coupling of immunomagnetic enrichment of bacteria with paper-based platform. AU - Ilhan,Hasan, AU - Guven,Burcu, AU - Dogan,Uzeyir, AU - Torul,Hilal, AU - Evran,Sefika, AU - Çetin,Demet, AU - Suludere,Zekiye, AU - Saglam,Necdet, AU - Boyaci,İsmail Hakki, AU - Tamer,Ugur, Y1 - 2019/04/09/ PY - 2019/01/30/received PY - 2019/04/04/revised PY - 2019/04/06/accepted PY - 2019/5/25/entrez PY - 2019/5/28/pubmed PY - 2019/6/18/medline KW - Escherichia coli (E. coli) KW - Lateral flow immunoassay (LFIA) KW - Surface enhanced Raman spectroscopy (SERS) SP - 245 EP - 252 JF - Talanta JO - Talanta VL - 201 N2 - In this study, the coupling of magnetic enrichment of bacteria from real samples with rapid surface enhanced Raman spectroscopy (SERS) detection was reported. The selective isolation and enrichment for the model bacteria Escherichia coli (E. coli) was performed using E. coli (primary) antibody bound-magnetic gold (Fe3O4@Au) nanoparticles. Following isolation and enrichment, the rennet enzyme was used to cleave of casein modified Fe3O4/Au-PEI nanoparticles from primary antibody-bound bacteria to prevent the nanoparticle aggregation and provide the movement of bacteria on nitrocellulose membrane. In the first part of the study, optimization studies were carried out namely; the amounts of gold nanoparticles (AuNPs), polyethyleneimine coated magnetic gold (Fe3O4/Au-PEI) nanoparticles, casein and rennet enzyme. The SERS signals of DTNB (5,5'-Dithiobis(2-nitrobenzoic acid)) molecule were collected on the test line and a calibration curve was plotted by using signal intensities. The correlation between the concentration of E. coli and SERS signal was found to be linear within the range of 10[1]-10[7] cfu/mL (R[2] = 0.984, LOD = 0.52 cfu/mL and LOQ = 1.57 cfu/mL). The selectivity of the paper-based lateral flow immunoassay (LFIA) was examined with Bacillus subtilis (B. subtilis), Micrococcus luteus (M. luteus), Salmonella enteritidis (S. enteritidis) which did not produce any significant response compared with E. coli measurement. Finally, the developed paper-based LFIA was tested with urine and milk samples. The obtained SERS results were compared with a plate counting method results which were in a good accordance. The developed method was found as rapid and sensitive to E. coli with a total analysis time of less than 60 min. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/31122419/The_coupling_of_immunomagnetic_enrichment_of_bacteria_with_paper_based_platform_ DB - PRIME DP - Unbound Medicine ER -