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Binding of an X-Specific Condensin Correlates with a Reduction in Active Histone Modifications at Gene Regulatory Elements.
Genetics 2019; 212(3):729-742G

Abstract

Condensins are evolutionarily conserved protein complexes that are required for chromosome segregation during cell division and genome organization during interphase. In Caenorhabditis elegans, a specialized condensin, which forms the core of the dosage compensation complex (DCC), binds to and represses X chromosome transcription. Here, we analyzed DCC localization and the effect of DCC depletion on histone modifications, transcription factor binding, and gene expression using chromatin immunoprecipitation sequencing and mRNA sequencing. Across the X, the DCC accumulates at accessible gene regulatory sites in active chromatin and not heterochromatin. The DCC is required for reducing the levels of activating histone modifications, including H3K4me3 and H3K27ac, but not repressive modification H3K9me3. In X-to-autosome fusion chromosomes, DCC spreading into the autosomal sequences locally reduces gene expression, thus establishing a direct link between DCC binding and repression. Together, our results indicate that DCC-mediated transcription repression is associated with a reduction in the activity of X chromosomal gene regulatory elements.

Authors+Show Affiliations

Department of Biology, Center for Genomics and Systems Biology, New York University, New York 10003.Department of Biology, Center for Genomics and Systems Biology, New York University, New York 10003.Department of Biology, Center for Genomics and Systems Biology, New York University, New York 10003.Department of Biology, Center for Genomics and Systems Biology, New York University, New York 10003.Department of Biology, Center for Genomics and Systems Biology, New York University, New York 10003.Department of Biology, Center for Genomics and Systems Biology, New York University, New York 10003.Department of Biology, Center for Genomics and Systems Biology, New York University, New York 10003.Department of Biology, Center for Genomics and Systems Biology, New York University, New York 10003 se71@nyu.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

31123040

Citation

Street, Lena Annika, et al. "Binding of an X-Specific Condensin Correlates With a Reduction in Active Histone Modifications at Gene Regulatory Elements." Genetics, vol. 212, no. 3, 2019, pp. 729-742.
Street LA, Morao AK, Winterkorn LH, et al. Binding of an X-Specific Condensin Correlates with a Reduction in Active Histone Modifications at Gene Regulatory Elements. Genetics. 2019;212(3):729-742.
Street, L. A., Morao, A. K., Winterkorn, L. H., Jiao, C. Y., Albritton, S. E., Sadic, M., ... Ercan, S. (2019). Binding of an X-Specific Condensin Correlates with a Reduction in Active Histone Modifications at Gene Regulatory Elements. Genetics, 212(3), pp. 729-742. doi:10.1534/genetics.119.302254.
Street LA, et al. Binding of an X-Specific Condensin Correlates With a Reduction in Active Histone Modifications at Gene Regulatory Elements. Genetics. 2019;212(3):729-742. PubMed PMID: 31123040.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Binding of an X-Specific Condensin Correlates with a Reduction in Active Histone Modifications at Gene Regulatory Elements. AU - Street,Lena Annika, AU - Morao,Ana Karina, AU - Winterkorn,Lara Heermans, AU - Jiao,Chen-Yu, AU - Albritton,Sarah Elizabeth, AU - Sadic,Mohammed, AU - Kramer,Maxwell, AU - Ercan,Sevinç, Y1 - 2019/05/22/ PY - 2019/01/22/received PY - 2019/05/20/accepted PY - 2020/07/01/pmc-release PY - 2019/5/28/pubmed PY - 2019/5/28/medline PY - 2019/5/25/entrez KW - C. elegans KW - X chromosome KW - chromatin KW - condensin KW - dosage compensation KW - gene expression KW - gene regulation KW - histone modifications KW - transcription SP - 729 EP - 742 JF - Genetics JO - Genetics VL - 212 IS - 3 N2 - Condensins are evolutionarily conserved protein complexes that are required for chromosome segregation during cell division and genome organization during interphase. In Caenorhabditis elegans, a specialized condensin, which forms the core of the dosage compensation complex (DCC), binds to and represses X chromosome transcription. Here, we analyzed DCC localization and the effect of DCC depletion on histone modifications, transcription factor binding, and gene expression using chromatin immunoprecipitation sequencing and mRNA sequencing. Across the X, the DCC accumulates at accessible gene regulatory sites in active chromatin and not heterochromatin. The DCC is required for reducing the levels of activating histone modifications, including H3K4me3 and H3K27ac, but not repressive modification H3K9me3. In X-to-autosome fusion chromosomes, DCC spreading into the autosomal sequences locally reduces gene expression, thus establishing a direct link between DCC binding and repression. Together, our results indicate that DCC-mediated transcription repression is associated with a reduction in the activity of X chromosomal gene regulatory elements. SN - 1943-2631 UR - https://www.unboundmedicine.com/medline/citation/31123040/Binding_of_an_X-Specific_Condensin_Correlates_with_a_Reduction_in_Active_Histone_Modifications_at_Gene_Regulatory_Elements L2 - http://www.genetics.org/cgi/pmidlookup?view=long&pmid=31123040 DB - PRIME DP - Unbound Medicine ER -