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DNA nanotetrahedron linked dual-aptamer based voltammetric aptasensor for cardiac troponin I using a magnetic metal-organic framework as a label.
Mikrochim Acta. 2019 05 23; 186(6):374.MA

Abstract

An ultrasensitive voltammetric aptasensor was constructed to analyze cardiac troponin I (cTnI). It is based on DNA nanotetrahedron (NTH) linked dual-aptamer (dAPT) and magnetic metal organic frameworks (mMOFs) of type Fe3O4@UiO-66. Firstly, the DNA NTH linked dAPT (Tro4 and Tro6) were immobilized on a gold electrode for improving the capture efficiency of cTnI. The novel mMOFs Fe3O4@UiO-66 was then decorated by Au@Pt nanoparticles (Au@PtNPs), horseradish peroxidase (HRP), G-quadruplex/hemin (GQH) DNAzyme, and two types of aptamers to form signaling nanoprobes. In the presence of cTnI, an aptamer-protein-nanoprobe sandwich-type structure is formed. Afterward, the nanoprobes including enzyme, GQH DNAzyme and Fe3O4@UiO-66/Au@PtNP were utilized to catalyze the oxidation of hydroquinone by hydrogen peroxide for the electrochemical signals amplification, typically at a working potential of -0.1 V (vs. Ag/AgCl). The voltammetric signal increases linearly in the 0.01 to 100 ng·mL-1 cTnI concentration range, and the detection limit is 5.7 pg·mL-1. Graphical abstract An ultrasensitive voltammetric aptasensor was constructed to analyze cardiac troponin I (cTnI) based on DNA nanotetrahedron linked dual-aptamer and magnetic metal organic frameworks of type Fe3O4@UiO-66. The results indicated the aptasensor has a wide linear response range (0.01 to 100 ng/mL) and low detection limit (5.74 pg/mL) for cTnI. GE: gold electrode; MCH: 6-Mmercapto-1-hexanol; HRP: horseradish peroxidase; HQ: hydroquinone; BQ: benzoquinone.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Luo Z
School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, 510006, China.
Sun D
School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, 510006, China. sundp@gdpu.edu.cn. Center for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou, 510006, China. sundp@gdpu.edu.cn.
Tong Y
Department of Pharmacy, Guangdong Second Provincial General Hospital, Guangzhou, 510317, China.
Zhong Y
East Campus Lab Center, Sun Yat-Sen University, Guangzhou, 510006, China.
Chen Z
School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, 510006, China. chenzg@mail.sysu.edu.cn.

MeSH

Aptamers, NucleotideArmoraciaBase SequenceBiosensing TechniquesDNA, CatalyticElectrochemical TechniquesG-QuadruplexesGoldHeminHorseradish PeroxidaseHumansHydrogen PeroxideHydroquinonesLimit of DetectionMagnetite NanoparticlesMetal-Organic FrameworksOxidation-ReductionReproducibility of ResultsSilverTroponin I

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31123904

Citation

Luo, Zibin, et al. "DNA Nanotetrahedron Linked Dual-aptamer Based Voltammetric Aptasensor for Cardiac Troponin I Using a Magnetic Metal-organic Framework as a Label." Mikrochimica Acta, vol. 186, no. 6, 2019, p. 374.
Luo Z, Sun D, Tong Y, et al. DNA nanotetrahedron linked dual-aptamer based voltammetric aptasensor for cardiac troponin I using a magnetic metal-organic framework as a label. Mikrochim Acta. 2019;186(6):374.
Luo, Z., Sun, D., Tong, Y., Zhong, Y., & Chen, Z. (2019). DNA nanotetrahedron linked dual-aptamer based voltammetric aptasensor for cardiac troponin I using a magnetic metal-organic framework as a label. Mikrochimica Acta, 186(6), 374. https://doi.org/10.1007/s00604-019-3470-1
Luo Z, et al. DNA Nanotetrahedron Linked Dual-aptamer Based Voltammetric Aptasensor for Cardiac Troponin I Using a Magnetic Metal-organic Framework as a Label. Mikrochim Acta. 2019 05 23;186(6):374. PubMed PMID: 31123904.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA nanotetrahedron linked dual-aptamer based voltammetric aptasensor for cardiac troponin I using a magnetic metal-organic framework as a label. AU - Luo,Zibin, AU - Sun,Duanping, AU - Tong,Yanli, AU - Zhong,Yongsheng, AU - Chen,Zuanguang, Y1 - 2019/05/23/ PY - 2019/01/21/received PY - 2019/04/29/accepted PY - 2019/5/25/entrez PY - 2019/5/28/pubmed PY - 2020/3/25/medline KW - Biosensor KW - DNA nanostructure KW - Dual aptamer KW - Fe3O4@UiO-66 KW - cTnI SP - 374 EP - 374 JF - Mikrochimica acta JO - Mikrochim Acta VL - 186 IS - 6 N2 - An ultrasensitive voltammetric aptasensor was constructed to analyze cardiac troponin I (cTnI). It is based on DNA nanotetrahedron (NTH) linked dual-aptamer (dAPT) and magnetic metal organic frameworks (mMOFs) of type Fe3O4@UiO-66. Firstly, the DNA NTH linked dAPT (Tro4 and Tro6) were immobilized on a gold electrode for improving the capture efficiency of cTnI. The novel mMOFs Fe3O4@UiO-66 was then decorated by Au@Pt nanoparticles (Au@PtNPs), horseradish peroxidase (HRP), G-quadruplex/hemin (GQH) DNAzyme, and two types of aptamers to form signaling nanoprobes. In the presence of cTnI, an aptamer-protein-nanoprobe sandwich-type structure is formed. Afterward, the nanoprobes including enzyme, GQH DNAzyme and Fe3O4@UiO-66/Au@PtNP were utilized to catalyze the oxidation of hydroquinone by hydrogen peroxide for the electrochemical signals amplification, typically at a working potential of -0.1 V (vs. Ag/AgCl). The voltammetric signal increases linearly in the 0.01 to 100 ng·mL-1 cTnI concentration range, and the detection limit is 5.7 pg·mL-1. Graphical abstract An ultrasensitive voltammetric aptasensor was constructed to analyze cardiac troponin I (cTnI) based on DNA nanotetrahedron linked dual-aptamer and magnetic metal organic frameworks of type Fe3O4@UiO-66. The results indicated the aptasensor has a wide linear response range (0.01 to 100 ng/mL) and low detection limit (5.74 pg/mL) for cTnI. GE: gold electrode; MCH: 6-Mmercapto-1-hexanol; HRP: horseradish peroxidase; HQ: hydroquinone; BQ: benzoquinone. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/31123904/DNA_nanotetrahedron_linked_dual_aptamer_based_voltammetric_aptasensor_for_cardiac_troponin_I_using_a_magnetic_metal_organic_framework_as_a_label_ DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓10 ↑23]

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