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Root extract of Prunella vulgaris inhibits in vitro and in vivo carcinogenesis in MCF-5 human breast carcinoma via suppression of angiogenesis, induction of apoptosis, cell cycle arrest and modulation of PI3K/AKT signalling pathway.
J BUON. 2019 Mar-Apr; 24(2):549-554.JB

Abstract

PURPOSE

In this study we examined the anticancer effects of methanolic root extract of Prunella Vulgaris (PVE) against the MCF-5 breast cancer (BC) cell line along with its mode of action.

METHODS

The proliferation rate of the MCF-5 cells was assessed by MTT assay. Apoptosis was confirmed by acridine orange (AO)/ethidium bromide (EB) and annexin V/propidium iodide (PI) staining. DNA damage was checked by comet assay. Cell cycle analysis was performed by flow cytometry. Protein expression was determined by western blotting. In vivo evaluation of the extract was carried out in xenografted tumor mice models.

RESULTS

PVE inhibited the growth of the MCF-5 cells and exhibited an IC50 value of 25 µg/ml. The investigation of underlying mechanism revealed that PVE triggered apoptotic cell death of the MCF-5 cells which was also associated with enhancement of the expression of Bax and decrease in the expression of Bcl-2. PVE also caused arrest of the cells in the G2/M phase of the cell cycle and also exerted the anti-angiogenic effects. In vivo evaluation of PVE showed that it could inhibit the tumor weight and volume, suggestive of the anticancer potential of PVE.

CONCLUSION

The root extract of Prunella vulgaris in this study was shown to exert potent anticancer effects in MCF-7 human BC cells both in vitro and in vivo, accompanied with apoptosis induction, inhibition of angiogenesis, cell cycle arrest, and modulation of PI3K/AKT signaling pathway.

Authors+Show Affiliations

The Department of Modern and Traditional Medicine, The Tumor Hospital of Qingdao, Qingdao, 266000, China.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31128004

Citation

Gao, Wei, and Hua Liang Yanlei Li Yuanyuan Liu Yanxia Xu. "Root Extract of Prunella Vulgaris Inhibits in Vitro and in Vivo Carcinogenesis in MCF-5 Human Breast Carcinoma Via Suppression of Angiogenesis, Induction of Apoptosis, Cell Cycle Arrest and Modulation of PI3K/AKT Signalling Pathway." Journal of B.U.ON. : Official Journal of the Balkan Union of Oncology, vol. 24, no. 2, 2019, pp. 549-554.
Gao W, Xu HLYLYLY. Root extract of Prunella vulgaris inhibits in vitro and in vivo carcinogenesis in MCF-5 human breast carcinoma via suppression of angiogenesis, induction of apoptosis, cell cycle arrest and modulation of PI3K/AKT signalling pathway. J BUON. 2019;24(2):549-554.
Gao, W., & Xu, H. L. Y. L. Y. L. Y. (2019). Root extract of Prunella vulgaris inhibits in vitro and in vivo carcinogenesis in MCF-5 human breast carcinoma via suppression of angiogenesis, induction of apoptosis, cell cycle arrest and modulation of PI3K/AKT signalling pathway. Journal of B.U.ON. : Official Journal of the Balkan Union of Oncology, 24(2), 549-554.
Gao W, Xu HLYLYLY. Root Extract of Prunella Vulgaris Inhibits in Vitro and in Vivo Carcinogenesis in MCF-5 Human Breast Carcinoma Via Suppression of Angiogenesis, Induction of Apoptosis, Cell Cycle Arrest and Modulation of PI3K/AKT Signalling Pathway. J BUON. 2019 Mar-Apr;24(2):549-554. PubMed PMID: 31128004.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Root extract of Prunella vulgaris inhibits in vitro and in vivo carcinogenesis in MCF-5 human breast carcinoma via suppression of angiogenesis, induction of apoptosis, cell cycle arrest and modulation of PI3K/AKT signalling pathway. AU - Gao,Wei, AU - Xu,Hua Liang Yanlei Li Yuanyuan Liu Yanxia, PY - 2019/5/26/entrez PY - 2019/5/28/pubmed PY - 2019/12/28/medline SP - 549 EP - 554 JF - Journal of B.U.ON. : official journal of the Balkan Union of Oncology JO - J BUON VL - 24 IS - 2 N2 - PURPOSE: In this study we examined the anticancer effects of methanolic root extract of Prunella Vulgaris (PVE) against the MCF-5 breast cancer (BC) cell line along with its mode of action. METHODS: The proliferation rate of the MCF-5 cells was assessed by MTT assay. Apoptosis was confirmed by acridine orange (AO)/ethidium bromide (EB) and annexin V/propidium iodide (PI) staining. DNA damage was checked by comet assay. Cell cycle analysis was performed by flow cytometry. Protein expression was determined by western blotting. In vivo evaluation of the extract was carried out in xenografted tumor mice models. RESULTS: PVE inhibited the growth of the MCF-5 cells and exhibited an IC50 value of 25 µg/ml. The investigation of underlying mechanism revealed that PVE triggered apoptotic cell death of the MCF-5 cells which was also associated with enhancement of the expression of Bax and decrease in the expression of Bcl-2. PVE also caused arrest of the cells in the G2/M phase of the cell cycle and also exerted the anti-angiogenic effects. In vivo evaluation of PVE showed that it could inhibit the tumor weight and volume, suggestive of the anticancer potential of PVE. CONCLUSION: The root extract of Prunella vulgaris in this study was shown to exert potent anticancer effects in MCF-7 human BC cells both in vitro and in vivo, accompanied with apoptosis induction, inhibition of angiogenesis, cell cycle arrest, and modulation of PI3K/AKT signaling pathway. SN - 2241-6293 UR - https://www.unboundmedicine.com/medline/citation/31128004/Root_extract_of_Prunella_vulgaris_inhibits_in_vitro_and_in_vivo_carcinogenesis_in_MCF_5_human_breast_carcinoma_via_suppression_of_angiogenesis_induction_of_apoptosis_cell_cycle_arrest_and_modulation_of_PI3K/AKT_signalling_pathway_ L2 - https://www.jbuon.com/archive/24-2-549.pdf DB - PRIME DP - Unbound Medicine ER -