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Evidence of exposure to Coxiella burnetii in neotropical free-living cervids in South America.
Acta Trop. 2019 Sep; 197:105037.AT

Abstract

Coxiella burnetii (order Legionellales, family Coxiellaceae), the etiological agent of Q fever, is a pleomorphic, obligate Gram-negative intracellular bacillococcus that can infect humans and animals. Among the mammals hosting this agent, both domestic and wild ruminants are of particular economic and public health importance. Ticks and other hematophagous arthropods or aerosols are incriminated in the transmission between reservoirs and susceptible hosts. This study used serological and molecular methods to investigate the C. burnetii occurrence in blood samples from free-living deer (143 Blastocerus dichotomus, 27 Mazama gouazoubira, 4 M. bororo, 3 M. americana, and 11 Ozotocerus bezoarticus) sampled in Mato-Grosso do Sul, São Paulo, Goiás and Paraná states in Brazil. The DNA extracted from the blood samples of 188 cervids was submitted to nested (n) PCR for C. burnetii based on the IS1111 repetitive insertion element of the heat shock protein (htpAB) gene. Additionally, 169 serum samples were submitted to Indirect Immunofluorescence Assay (IFAT) to detect Ig antibodies to C. burnetii. The nPCR results indicated that all blood samples were negative, evidencing the absence of circulating C. burnetii DNA in cervids from the studied regions or, alternatively, the C. burnetii DNA concentration in the deer blood samples was below the threshold of the used PCR technique. On the other hand, 5.32% (9/169) of deer were seropositive for C. burnetii by IFAT, with titers ranging from 256 and 16,384. In conclusion, the present work showed the first evidence of exposure of free-living deer to C. burnetii in Brazil.

Authors+Show Affiliations

Programa de Pós-graduação em Microbiologia Agropecuária, Universidade Estadual Paulista Júlio de Mesquita Filho, Jaboticabal, São Paulo, Brazil; Departamento de Patologia Veterinária, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Jaboticabal, São Paulo, Brazil.Departamento de Zootecnia, Universidade Estadual Paulista - Júlio de Mesquita Filho, Jaboticabal, São Paulo, Brazil.Departamento de Medicina Veterinária Preventiva e Saúde Animal, Universidade de São Paulo, São Paulo, Brazil.Departamento de Patologia Veterinária, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Jaboticabal, São Paulo, Brazil.Departamento de Patologia Veterinária, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Jaboticabal, São Paulo, Brazil.Departamento de Patologia Veterinária, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Jaboticabal, São Paulo, Brazil.Departamento de Patologia Veterinária, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Jaboticabal, São Paulo, Brazil. Electronic address: mr.andre@unesp.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31128095

Citation

Zanatto, Diego Carlos de Souza, et al. "Evidence of Exposure to Coxiella Burnetii in Neotropical Free-living Cervids in South America." Acta Tropica, vol. 197, 2019, p. 105037.
Zanatto DCS, Duarte JMB, Labruna MB, et al. Evidence of exposure to Coxiella burnetii in neotropical free-living cervids in South America. Acta Trop. 2019;197:105037.
Zanatto, D. C. S., Duarte, J. M. B., Labruna, M. B., Tasso, J. B., Calchi, A. C., Machado, R. Z., & André, M. R. (2019). Evidence of exposure to Coxiella burnetii in neotropical free-living cervids in South America. Acta Tropica, 197, 105037. https://doi.org/10.1016/j.actatropica.2019.05.028
Zanatto DCS, et al. Evidence of Exposure to Coxiella Burnetii in Neotropical Free-living Cervids in South America. Acta Trop. 2019;197:105037. PubMed PMID: 31128095.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence of exposure to Coxiella burnetii in neotropical free-living cervids in South America. AU - Zanatto,Diego Carlos de Souza, AU - Duarte,José Maurício Barbanti, AU - Labruna,Marcelo Bahia, AU - Tasso,Júlia Banhareli, AU - Calchi,Ana Cláudia, AU - Machado,Rosangela Zacarias, AU - André,Marcos Rogério, Y1 - 2019/05/22/ PY - 2019/03/28/received PY - 2019/05/21/revised PY - 2019/05/21/accepted PY - 2019/5/28/pubmed PY - 2019/12/4/medline PY - 2019/5/26/entrez KW - Blastocerus dichotomus KW - Brazil KW - Deer KW - Mazama spp. KW - Q fever KW - Serology SP - 105037 EP - 105037 JF - Acta tropica JO - Acta Trop VL - 197 N2 - Coxiella burnetii (order Legionellales, family Coxiellaceae), the etiological agent of Q fever, is a pleomorphic, obligate Gram-negative intracellular bacillococcus that can infect humans and animals. Among the mammals hosting this agent, both domestic and wild ruminants are of particular economic and public health importance. Ticks and other hematophagous arthropods or aerosols are incriminated in the transmission between reservoirs and susceptible hosts. This study used serological and molecular methods to investigate the C. burnetii occurrence in blood samples from free-living deer (143 Blastocerus dichotomus, 27 Mazama gouazoubira, 4 M. bororo, 3 M. americana, and 11 Ozotocerus bezoarticus) sampled in Mato-Grosso do Sul, São Paulo, Goiás and Paraná states in Brazil. The DNA extracted from the blood samples of 188 cervids was submitted to nested (n) PCR for C. burnetii based on the IS1111 repetitive insertion element of the heat shock protein (htpAB) gene. Additionally, 169 serum samples were submitted to Indirect Immunofluorescence Assay (IFAT) to detect Ig antibodies to C. burnetii. The nPCR results indicated that all blood samples were negative, evidencing the absence of circulating C. burnetii DNA in cervids from the studied regions or, alternatively, the C. burnetii DNA concentration in the deer blood samples was below the threshold of the used PCR technique. On the other hand, 5.32% (9/169) of deer were seropositive for C. burnetii by IFAT, with titers ranging from 256 and 16,384. In conclusion, the present work showed the first evidence of exposure of free-living deer to C. burnetii in Brazil. SN - 1873-6254 UR - https://www.unboundmedicine.com/medline/citation/31128095/Evidence_of_exposure_to_Coxiella_burnetii_in_neotropical_free_living_cervids_in_South_America_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0001-706X(19)30414-0 DB - PRIME DP - Unbound Medicine ER -