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Proanthocyanidin-chitosan composite nanoparticles prevent bacterial invasion and colonization of gut epithelial cells by extra-intestinal pathogenic Escherichia coli.
Int J Biol Macromol. 2019 Aug 15; 135:630-636.IJ

Abstract

Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles. The PAC-CHT NPs were characterized by size, polydispersity, surface charge, morphology, and PAC content. PAC-CHT NPs bioactivity was measured by agglutination of extra-intestinal pathogenic Escherichia coli (ExPEC) and inhibition of gut epithelial cell invasion by ExPEC. Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV. PAC-CHT NPs ratio 30:1 agglutinated ExPEC and decreased the ability of ExPEC to invade epithelial cells in a dose-dependent manner. PAC-CHT NPs ratio 10:1 to 30:1 form stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections.

Authors+Show Affiliations

Material Sciences & Engineering, University of Wisconsin-Madison, 1415 Engineering Dr, Madison, WI 53706, United States; Reed Research Group, Department of Animal Science, University of Wisconsin-Madison, 1675 Observatory Dr, Madison, WI 53706, United States. Electronic address: alfaroviquez@wisc.edu.Reed Research Group, Department of Animal Science, University of Wisconsin-Madison, 1675 Observatory Dr, Madison, WI 53706, United States.Reed Research Group, Department of Animal Science, University of Wisconsin-Madison, 1675 Observatory Dr, Madison, WI 53706, United States.Reed Research Group, Department of Animal Science, University of Wisconsin-Madison, 1675 Observatory Dr, Madison, WI 53706, United States.Material Sciences & Engineering, University of Wisconsin-Madison, 1415 Engineering Dr, Madison, WI 53706, United States; Reed Research Group, Department of Animal Science, University of Wisconsin-Madison, 1675 Observatory Dr, Madison, WI 53706, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31128185

Citation

Alfaro-Viquez, Emilia, et al. "Proanthocyanidin-chitosan Composite Nanoparticles Prevent Bacterial Invasion and Colonization of Gut Epithelial Cells By Extra-intestinal Pathogenic Escherichia Coli." International Journal of Biological Macromolecules, vol. 135, 2019, pp. 630-636.
Alfaro-Viquez E, Esquivel-Alvarado D, Madrigal-Carballo S, et al. Proanthocyanidin-chitosan composite nanoparticles prevent bacterial invasion and colonization of gut epithelial cells by extra-intestinal pathogenic Escherichia coli. Int J Biol Macromol. 2019;135:630-636.
Alfaro-Viquez, E., Esquivel-Alvarado, D., Madrigal-Carballo, S., Krueger, C. G., & Reed, J. D. (2019). Proanthocyanidin-chitosan composite nanoparticles prevent bacterial invasion and colonization of gut epithelial cells by extra-intestinal pathogenic Escherichia coli. International Journal of Biological Macromolecules, 135, 630-636. https://doi.org/10.1016/j.ijbiomac.2019.04.170
Alfaro-Viquez E, et al. Proanthocyanidin-chitosan Composite Nanoparticles Prevent Bacterial Invasion and Colonization of Gut Epithelial Cells By Extra-intestinal Pathogenic Escherichia Coli. Int J Biol Macromol. 2019 Aug 15;135:630-636. PubMed PMID: 31128185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proanthocyanidin-chitosan composite nanoparticles prevent bacterial invasion and colonization of gut epithelial cells by extra-intestinal pathogenic Escherichia coli. AU - Alfaro-Viquez,Emilia, AU - Esquivel-Alvarado,Daniel, AU - Madrigal-Carballo,Sergio, AU - Krueger,Christian G, AU - Reed,Jess D, Y1 - 2019/05/23/ PY - 2019/02/10/received PY - 2019/04/24/revised PY - 2019/04/24/accepted PY - 2019/5/28/pubmed PY - 2019/5/28/medline PY - 2019/5/26/entrez KW - Agglutination KW - Chitosan KW - Extra-intestinal pathogenic Escherichia coli KW - Invasion KW - Proanthocyanidins SP - 630 EP - 636 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 135 N2 - Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles. The PAC-CHT NPs were characterized by size, polydispersity, surface charge, morphology, and PAC content. PAC-CHT NPs bioactivity was measured by agglutination of extra-intestinal pathogenic Escherichia coli (ExPEC) and inhibition of gut epithelial cell invasion by ExPEC. Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV. PAC-CHT NPs ratio 30:1 agglutinated ExPEC and decreased the ability of ExPEC to invade epithelial cells in a dose-dependent manner. PAC-CHT NPs ratio 10:1 to 30:1 form stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/31128185/Proanthocyanidin_chitosan_composite_nanoparticles_prevent_bacterial_invasion_and_colonization_of_gut_epithelial_cells_by_extra_intestinal_pathogenic_Escherichia_coli_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(19)31016-5 DB - PRIME DP - Unbound Medicine ER -